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Effects of KH-204 on the expression of heat shock protein 70 and germ cell apoptosis in infertility rat models.

Bae WJ, Ha US, Kim KS, Kim SJ, Cho HJ, Hong SH, Lee JY, Wang Z, Hwang SY, Kim SW - BMC Complement Altern Med (2014)

Bottom Line: We investigated the antioxidant effects of the modified Ojayeonjonghwan (KH-204) in a rat model of cryptorchidism.Treatment with the multi-herbal medicine KH-204 (1) increased the mean weight of the cryptorchid testes; (2) restored sperm counts, motility and germinal cell layer thickness; (3) decreased levels of 8-OHdG and increased levels of SOD; and (4) decreased HSP70 levels and apoptosis.KH-204 reduces the oxidative stress in an experimental rat model of cryptorchidism, and it may alleviate HSP expression and germ cell apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 137-701, Republic of Korea. ksw1227@catholic.ac.kr.

ABSTRACT

Background: Idiopathic infertility is a significant number of causes of male infertility. Empirical treatments are used for idiopathic male infertility, and antioxidant supplementation is a kind of management of oxidative stress related infertility. We investigated the antioxidant effects of the modified Ojayeonjonghwan (KH-204) in a rat model of cryptorchidism.

Method: Male rats were divided into four groups (n = 8 in each): a normal control group, a cryptorchidism-induced control group and two cryptorchidism-induced groups treated p.o. with either 200 or 400 mg/kg, KH-204 for 4 weeks. The testes and epididymides from rats in all groups were removed, weighed and subjected to histological examination and semen analysis after surgery. Oxidative stress was assessed by measuring 8-hydroxy-20-deoxyguanosine (8-OHdG), superoxide dismutase (SOD) and heat shock protein (HSP) levels. Apoptosis was determined using a terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling assay.

Results: Treatment with the multi-herbal medicine KH-204 (1) increased the mean weight of the cryptorchid testes; (2) restored sperm counts, motility and germinal cell layer thickness; (3) decreased levels of 8-OHdG and increased levels of SOD; and (4) decreased HSP70 levels and apoptosis.

Conclusions: KH-204 reduces the oxidative stress in an experimental rat model of cryptorchidism, and it may alleviate HSP expression and germ cell apoptosis.

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Comparison of the expression levels of HSP70 (A) and densitometric analysis relative to beta-actin of HSP70 (B). *, p < 0.05 as compared with the Control group. **, p < 0.05 as compared with the Crypto-control group. By analysis of variance test.
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Fig4: Comparison of the expression levels of HSP70 (A) and densitometric analysis relative to beta-actin of HSP70 (B). *, p < 0.05 as compared with the Control group. **, p < 0.05 as compared with the Crypto-control group. By analysis of variance test.

Mentions: Significant increases in HSP70 were exhibited in the Crypto-control group, but KH-204 administration significantly decreased HSP70 levels in the testes of experimental rats (Figure 4). HSP70 levels in the Crypto-200 and Crypto-400 group were significantly decreased compared with those of the Crypto-control and were similar to those of Control rats.Figure 4


Effects of KH-204 on the expression of heat shock protein 70 and germ cell apoptosis in infertility rat models.

Bae WJ, Ha US, Kim KS, Kim SJ, Cho HJ, Hong SH, Lee JY, Wang Z, Hwang SY, Kim SW - BMC Complement Altern Med (2014)

Comparison of the expression levels of HSP70 (A) and densitometric analysis relative to beta-actin of HSP70 (B). *, p < 0.05 as compared with the Control group. **, p < 0.05 as compared with the Crypto-control group. By analysis of variance test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4197240&req=5

Fig4: Comparison of the expression levels of HSP70 (A) and densitometric analysis relative to beta-actin of HSP70 (B). *, p < 0.05 as compared with the Control group. **, p < 0.05 as compared with the Crypto-control group. By analysis of variance test.
Mentions: Significant increases in HSP70 were exhibited in the Crypto-control group, but KH-204 administration significantly decreased HSP70 levels in the testes of experimental rats (Figure 4). HSP70 levels in the Crypto-200 and Crypto-400 group were significantly decreased compared with those of the Crypto-control and were similar to those of Control rats.Figure 4

Bottom Line: We investigated the antioxidant effects of the modified Ojayeonjonghwan (KH-204) in a rat model of cryptorchidism.Treatment with the multi-herbal medicine KH-204 (1) increased the mean weight of the cryptorchid testes; (2) restored sperm counts, motility and germinal cell layer thickness; (3) decreased levels of 8-OHdG and increased levels of SOD; and (4) decreased HSP70 levels and apoptosis.KH-204 reduces the oxidative stress in an experimental rat model of cryptorchidism, and it may alleviate HSP expression and germ cell apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 137-701, Republic of Korea. ksw1227@catholic.ac.kr.

ABSTRACT

Background: Idiopathic infertility is a significant number of causes of male infertility. Empirical treatments are used for idiopathic male infertility, and antioxidant supplementation is a kind of management of oxidative stress related infertility. We investigated the antioxidant effects of the modified Ojayeonjonghwan (KH-204) in a rat model of cryptorchidism.

Method: Male rats were divided into four groups (n = 8 in each): a normal control group, a cryptorchidism-induced control group and two cryptorchidism-induced groups treated p.o. with either 200 or 400 mg/kg, KH-204 for 4 weeks. The testes and epididymides from rats in all groups were removed, weighed and subjected to histological examination and semen analysis after surgery. Oxidative stress was assessed by measuring 8-hydroxy-20-deoxyguanosine (8-OHdG), superoxide dismutase (SOD) and heat shock protein (HSP) levels. Apoptosis was determined using a terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling assay.

Results: Treatment with the multi-herbal medicine KH-204 (1) increased the mean weight of the cryptorchid testes; (2) restored sperm counts, motility and germinal cell layer thickness; (3) decreased levels of 8-OHdG and increased levels of SOD; and (4) decreased HSP70 levels and apoptosis.

Conclusions: KH-204 reduces the oxidative stress in an experimental rat model of cryptorchidism, and it may alleviate HSP expression and germ cell apoptosis.

Show MeSH
Related in: MedlinePlus