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Evolution of multipartite mitochondrial genomes in the booklice of the genus Liposcelis (Psocoptera).

Chen SC, Wei DD, Shao R, Shi JX, Dou W, Wang JJ - BMC Genomics (2014)

Bottom Line: We found that these two species of booklice also have multipartite mt genomes, like L. bostrychophila, with the mt genes we identified on two chromosomes.Numerous pseudo mt genes and non-coding regions were found in the mt genomes of these two booklice, and account for 30% and 10% respectively of the entire length we sequenced.L. entomophila and L. paeta differ substantially from each other and from L. bostrychophila in gene content and gene arrangement in their mt chromosomes.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, P, R, China. jjwang7008@yahoo.com.

ABSTRACT

Background: The genus Liposcelis (Psocoptera: Troctomorpha) has more than 120 species with a worldwide distribution and they pose a risk for global food security. The organization of mitochondrial (mt) genomes varies between the two species of booklice investigated in the genus Liposcelis. Liposcelis decolor has its mt genes on a single chromosome, like most other insects; L. bostrychophila, however, has a multipartite mt genome with genes on two chromosomes.

Results: To understand how multipartite mt genome organization evolved in the genus Liposcelis, we sequenced the mt genomes of L. entomophila and L. paeta in this study. We found that these two species of booklice also have multipartite mt genomes, like L. bostrychophila, with the mt genes we identified on two chromosomes. Numerous pseudo mt genes and non-coding regions were found in the mt genomes of these two booklice, and account for 30% and 10% respectively of the entire length we sequenced. In L. bostrychophila, the mt genes are distributed approximately equally between the two chromosomes. In L. entomophila and L. paeta, however, one mt chromosome has most of the genes we identified whereas the other chromosome has largely pseudogenes and non-coding regions. L. entomophila and L. paeta differ substantially from each other and from L. bostrychophila in gene content and gene arrangement in their mt chromosomes.

Conclusions: Our results indicate unusually fast evolution in mt genome organization in the booklice of the genus Liposcelis, and reveal different patterns of mt genome fragmentation among L. bostrychophila, L. entomophila and L. paeta.

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Alignment of nucleotide sequences in the non-coding regions of the mitochondrial genomes ofLiposcelis entomophila(A) andLiposcelis paeta(B). The green highlight shows the concensus sequence of the three non-coding regions in Liposcelis entomophila(A) and the two in L. paeta(B), yellow shows the concensus sequence of NCRI-1 and NCRII-2, purple shows that of NCRI-3 and NCRII-2 and blue shows that of NCRI-1 and NCRI-3 in L. entomophila(A).
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Fig5: Alignment of nucleotide sequences in the non-coding regions of the mitochondrial genomes ofLiposcelis entomophila(A) andLiposcelis paeta(B). The green highlight shows the concensus sequence of the three non-coding regions in Liposcelis entomophila(A) and the two in L. paeta(B), yellow shows the concensus sequence of NCRI-1 and NCRII-2, purple shows that of NCRI-3 and NCRII-2 and blue shows that of NCRI-1 and NCRI-3 in L. entomophila(A).

Mentions: Non-coding sequences also account for large proportions of the mt chromosomes of L. entomophila and L. paeta. The non-coding sequences are 8,912 bp and 6,391 bp long, in total, for L. entomophila and L. paeta, and account for 36.72% and 29.29% of the entire length of their mt chromosomes. There are 22 and 13 non-coding sequences that are longer than 100 bp in the mt chromosomes of L. entomophila and L. paeta, respectively. For both L. entomophila and L. paeta, pseudogenes and non-coding sequences are largely on one of the mt chromosomes (chromosome II), whereas coding sequences are on the other chromosome (chromosome I) (Figures 2 and 4). Intriguingly, partial sequences are shared by three or two non-coding regions in L. entomophila and L. paeta. Three non-coding regions (NCRI-1, NCRI-3 and NCRII-2) of L. entomophila, each two of the three share a consistent sequence, and a partial sequence is common in all of the three. In L. paeta, two non-coding regions from mt chromosome II (NCRII-5 and NCRII-10) contain the same 286 bp sequence with two nucleotides change (Figure 5).Figure 4


Evolution of multipartite mitochondrial genomes in the booklice of the genus Liposcelis (Psocoptera).

Chen SC, Wei DD, Shao R, Shi JX, Dou W, Wang JJ - BMC Genomics (2014)

Alignment of nucleotide sequences in the non-coding regions of the mitochondrial genomes ofLiposcelis entomophila(A) andLiposcelis paeta(B). The green highlight shows the concensus sequence of the three non-coding regions in Liposcelis entomophila(A) and the two in L. paeta(B), yellow shows the concensus sequence of NCRI-1 and NCRII-2, purple shows that of NCRI-3 and NCRII-2 and blue shows that of NCRI-1 and NCRI-3 in L. entomophila(A).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4197233&req=5

Fig5: Alignment of nucleotide sequences in the non-coding regions of the mitochondrial genomes ofLiposcelis entomophila(A) andLiposcelis paeta(B). The green highlight shows the concensus sequence of the three non-coding regions in Liposcelis entomophila(A) and the two in L. paeta(B), yellow shows the concensus sequence of NCRI-1 and NCRII-2, purple shows that of NCRI-3 and NCRII-2 and blue shows that of NCRI-1 and NCRI-3 in L. entomophila(A).
Mentions: Non-coding sequences also account for large proportions of the mt chromosomes of L. entomophila and L. paeta. The non-coding sequences are 8,912 bp and 6,391 bp long, in total, for L. entomophila and L. paeta, and account for 36.72% and 29.29% of the entire length of their mt chromosomes. There are 22 and 13 non-coding sequences that are longer than 100 bp in the mt chromosomes of L. entomophila and L. paeta, respectively. For both L. entomophila and L. paeta, pseudogenes and non-coding sequences are largely on one of the mt chromosomes (chromosome II), whereas coding sequences are on the other chromosome (chromosome I) (Figures 2 and 4). Intriguingly, partial sequences are shared by three or two non-coding regions in L. entomophila and L. paeta. Three non-coding regions (NCRI-1, NCRI-3 and NCRII-2) of L. entomophila, each two of the three share a consistent sequence, and a partial sequence is common in all of the three. In L. paeta, two non-coding regions from mt chromosome II (NCRII-5 and NCRII-10) contain the same 286 bp sequence with two nucleotides change (Figure 5).Figure 4

Bottom Line: We found that these two species of booklice also have multipartite mt genomes, like L. bostrychophila, with the mt genes we identified on two chromosomes.Numerous pseudo mt genes and non-coding regions were found in the mt genomes of these two booklice, and account for 30% and 10% respectively of the entire length we sequenced.L. entomophila and L. paeta differ substantially from each other and from L. bostrychophila in gene content and gene arrangement in their mt chromosomes.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, P, R, China. jjwang7008@yahoo.com.

ABSTRACT

Background: The genus Liposcelis (Psocoptera: Troctomorpha) has more than 120 species with a worldwide distribution and they pose a risk for global food security. The organization of mitochondrial (mt) genomes varies between the two species of booklice investigated in the genus Liposcelis. Liposcelis decolor has its mt genes on a single chromosome, like most other insects; L. bostrychophila, however, has a multipartite mt genome with genes on two chromosomes.

Results: To understand how multipartite mt genome organization evolved in the genus Liposcelis, we sequenced the mt genomes of L. entomophila and L. paeta in this study. We found that these two species of booklice also have multipartite mt genomes, like L. bostrychophila, with the mt genes we identified on two chromosomes. Numerous pseudo mt genes and non-coding regions were found in the mt genomes of these two booklice, and account for 30% and 10% respectively of the entire length we sequenced. In L. bostrychophila, the mt genes are distributed approximately equally between the two chromosomes. In L. entomophila and L. paeta, however, one mt chromosome has most of the genes we identified whereas the other chromosome has largely pseudogenes and non-coding regions. L. entomophila and L. paeta differ substantially from each other and from L. bostrychophila in gene content and gene arrangement in their mt chromosomes.

Conclusions: Our results indicate unusually fast evolution in mt genome organization in the booklice of the genus Liposcelis, and reveal different patterns of mt genome fragmentation among L. bostrychophila, L. entomophila and L. paeta.

Show MeSH
Related in: MedlinePlus