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Molecular survey of Babesia gibsoni using Haemaphysalis longicornis collected from dogs and cats in Japan.

Iwakami S, Ichikawa Y, Inokuma H - J. Vet. Med. Sci. (2014)

Bottom Line: A total of 1,341 H. longicornis, including 305 females, 14 males, 332 nymphs and 690 larvae (153 pools) from 44 prefectures, were examined by B. gibsoni-targeted PCR.Partial sequence analysis revealed that 12 of 13 positive samples sequenced, including samples from Tottori, Hiroshima, Yamaguchi, Tokushima, Ehime and Oita prefectures (all in western Japan), were identical to B. gibsoni, and 1 sample from Kyoto Prefecture was most closely related to a Babesia species recently detected from feral raccoons in Hokkaido.H. longicornis is a candidate for transmission vector tick of the new Babesia species.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Veterinary Sciences, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.

ABSTRACT
A nationwide survey of Babesia gibsoni using Haemaphysalis longicornis collected from dogs and cats in Japan was conducted using molecular methods. A total of 1,341 H. longicornis, including 305 females, 14 males, 332 nymphs and 690 larvae (153 pools) from 44 prefectures, were examined by B. gibsoni-targeted PCR. Partial sequence analysis revealed that 12 of 13 positive samples sequenced, including samples from Tottori, Hiroshima, Yamaguchi, Tokushima, Ehime and Oita prefectures (all in western Japan), were identical to B. gibsoni, and 1 sample from Kyoto Prefecture was most closely related to a Babesia species recently detected from feral raccoons in Hokkaido. H. longicornis is a candidate for transmission vector tick of the new Babesia species.

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Phylogenetic relationship of Babesia spp. detected from 2 canine ticks(Tokushima-HL666L and Kyoto-HL623L) and the other Babesia spp. based on18S rRNA gene using an unrooted maximum-likelihood method (HKY model). B.gibsoni Asia-1 (AF175300), B. canis rossi (DQ111760),B. canis canis (AY072926), B. canis vogeli(AY371198), B. divergens (U16370), B. odocoilei(U16369), B. ovate (AY081192), Babesia sp. from aHokkaido brown bear (AB480557), Babesia sp. from feral raccoon MA361-1(AB251610), Babesia sp. from American mink NV-1 (AB649052) andBabesia sp. Akita 610 (AY190123) are shown. Scale bar represents 10%divergent.
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fig_001: Phylogenetic relationship of Babesia spp. detected from 2 canine ticks(Tokushima-HL666L and Kyoto-HL623L) and the other Babesia spp. based on18S rRNA gene using an unrooted maximum-likelihood method (HKY model). B.gibsoni Asia-1 (AF175300), B. canis rossi (DQ111760),B. canis canis (AY072926), B. canis vogeli(AY371198), B. divergens (U16370), B. odocoilei(U16369), B. ovate (AY081192), Babesia sp. from aHokkaido brown bear (AB480557), Babesia sp. from feral raccoon MA361-1(AB251610), Babesia sp. from American mink NV-1 (AB649052) andBabesia sp. Akita 610 (AY190123) are shown. Scale bar represents 10%divergent.

Mentions: A total of 13 tick samples removed from dogs in seven prefectures showed a single band of theappropriate size in the B. gibsoni-targeted PCR. None of the ticks collectedfrom cats gave positive results. Analysis of the partial sequences of the 13 PCR products(excluding the primer region) showed that 12 samples from Tottori, Hiroshima, Yamaguchi,Tokushima, Ehime and Oita prefectures were 100% identical to the registered sequences ofB. gibsoni isolates from Japan, such as B. gibsoni Asia-1(AF175300) (Fig. 1Fig. 1.


Molecular survey of Babesia gibsoni using Haemaphysalis longicornis collected from dogs and cats in Japan.

Iwakami S, Ichikawa Y, Inokuma H - J. Vet. Med. Sci. (2014)

Phylogenetic relationship of Babesia spp. detected from 2 canine ticks(Tokushima-HL666L and Kyoto-HL623L) and the other Babesia spp. based on18S rRNA gene using an unrooted maximum-likelihood method (HKY model). B.gibsoni Asia-1 (AF175300), B. canis rossi (DQ111760),B. canis canis (AY072926), B. canis vogeli(AY371198), B. divergens (U16370), B. odocoilei(U16369), B. ovate (AY081192), Babesia sp. from aHokkaido brown bear (AB480557), Babesia sp. from feral raccoon MA361-1(AB251610), Babesia sp. from American mink NV-1 (AB649052) andBabesia sp. Akita 610 (AY190123) are shown. Scale bar represents 10%divergent.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4197166&req=5

fig_001: Phylogenetic relationship of Babesia spp. detected from 2 canine ticks(Tokushima-HL666L and Kyoto-HL623L) and the other Babesia spp. based on18S rRNA gene using an unrooted maximum-likelihood method (HKY model). B.gibsoni Asia-1 (AF175300), B. canis rossi (DQ111760),B. canis canis (AY072926), B. canis vogeli(AY371198), B. divergens (U16370), B. odocoilei(U16369), B. ovate (AY081192), Babesia sp. from aHokkaido brown bear (AB480557), Babesia sp. from feral raccoon MA361-1(AB251610), Babesia sp. from American mink NV-1 (AB649052) andBabesia sp. Akita 610 (AY190123) are shown. Scale bar represents 10%divergent.
Mentions: A total of 13 tick samples removed from dogs in seven prefectures showed a single band of theappropriate size in the B. gibsoni-targeted PCR. None of the ticks collectedfrom cats gave positive results. Analysis of the partial sequences of the 13 PCR products(excluding the primer region) showed that 12 samples from Tottori, Hiroshima, Yamaguchi,Tokushima, Ehime and Oita prefectures were 100% identical to the registered sequences ofB. gibsoni isolates from Japan, such as B. gibsoni Asia-1(AF175300) (Fig. 1Fig. 1.

Bottom Line: A total of 1,341 H. longicornis, including 305 females, 14 males, 332 nymphs and 690 larvae (153 pools) from 44 prefectures, were examined by B. gibsoni-targeted PCR.Partial sequence analysis revealed that 12 of 13 positive samples sequenced, including samples from Tottori, Hiroshima, Yamaguchi, Tokushima, Ehime and Oita prefectures (all in western Japan), were identical to B. gibsoni, and 1 sample from Kyoto Prefecture was most closely related to a Babesia species recently detected from feral raccoons in Hokkaido.H. longicornis is a candidate for transmission vector tick of the new Babesia species.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Veterinary Sciences, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.

ABSTRACT
A nationwide survey of Babesia gibsoni using Haemaphysalis longicornis collected from dogs and cats in Japan was conducted using molecular methods. A total of 1,341 H. longicornis, including 305 females, 14 males, 332 nymphs and 690 larvae (153 pools) from 44 prefectures, were examined by B. gibsoni-targeted PCR. Partial sequence analysis revealed that 12 of 13 positive samples sequenced, including samples from Tottori, Hiroshima, Yamaguchi, Tokushima, Ehime and Oita prefectures (all in western Japan), were identical to B. gibsoni, and 1 sample from Kyoto Prefecture was most closely related to a Babesia species recently detected from feral raccoons in Hokkaido. H. longicornis is a candidate for transmission vector tick of the new Babesia species.

Show MeSH
Related in: MedlinePlus