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Full genome sequences of zebra-borne equine herpesvirus type 1 isolated from zebra, onager and Thomson's gazelle.

Guo X, Izume S, Okada A, Ohya K, Kimura T, Fukushi H - J. Vet. Med. Sci. (2014)

Bottom Line: The full genome sequences of the 3 strains were determined.Sequence data indicated that the EHV-1 isolated from a polar bear in Germany is one of the zebra-borne EHV-1 and not a recombinant virus.These results indicated that zebra-borne EHV-1 is a subtype of EHV-1.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.

ABSTRACT
A strain of equine herpesvirus type 1 (EHV-1) was isolated from zebra. This strain, called "zebra-borne EHV-1", was also isolated from an onager and a gazelle in zoological gardens in U.S.A. The full genome sequences of the 3 strains were determined. They shared 99% identities with each other, while they shared 98% and 95% identities with the horse derived EHV-1 and equine herpesvirus type 9, respectively. Sequence data indicated that the EHV-1 isolated from a polar bear in Germany is one of the zebra-borne EHV-1 and not a recombinant virus. These results indicated that zebra-borne EHV-1 is a subtype of EHV-1.

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Related in: MedlinePlus

(A) Nucleotide deletion from the tail part of ORF70 to the head part of ORF71. Thearrow in black indicates the original ORF70. The other arrow in dark grey indicates thetruncated tail part of ORF70 caused by deletion. (B) Amino acid sequence alignments ofthe tail part of ORF70 in Substrains 1 and 2. The amino acid sequences with underlineindicate the corresponding sequences shown in the panel A. The amino acid sequence initalic grey indicates the truncated tail of ORF70 caused by the deletion. The asterisks(*) indicate the placement of stop codon in the original nucleotide sequence. (C) PCRresults to confirm the presence of 2 viruses in T-616. PCR primers used were as follows:Zebra_71-F 5′-ccaacgtaccatcaagtgcggta-3′ and Zebra_71-R 5′-cgctggtactctcgtaggttgac-3′.PCR was examined by using PrimeSTAR Max Premix (TaKaRa Bio, Otsu, Japan) withamplification program as follows: the primary hold at 95°C for 4 min, 30 cycles of 98°C10 sec, 55°C 15 sec and 72°C 45 sec. Lanes were 100 bp ladder marker (1), T-616substrain 2 (2), T-616 substrain 1 (3), the original seed stock of T-616 (4) and 1 kbpladder marker (5). Expected sizes are 344 bps for T-616 substrain 2 and 2,058 bps forT-616 substrain 1.
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fig_002: (A) Nucleotide deletion from the tail part of ORF70 to the head part of ORF71. Thearrow in black indicates the original ORF70. The other arrow in dark grey indicates thetruncated tail part of ORF70 caused by deletion. (B) Amino acid sequence alignments ofthe tail part of ORF70 in Substrains 1 and 2. The amino acid sequences with underlineindicate the corresponding sequences shown in the panel A. The amino acid sequence initalic grey indicates the truncated tail of ORF70 caused by the deletion. The asterisks(*) indicate the placement of stop codon in the original nucleotide sequence. (C) PCRresults to confirm the presence of 2 viruses in T-616. PCR primers used were as follows:Zebra_71-F 5′-ccaacgtaccatcaagtgcggta-3′ and Zebra_71-R 5′-cgctggtactctcgtaggttgac-3′.PCR was examined by using PrimeSTAR Max Premix (TaKaRa Bio, Otsu, Japan) withamplification program as follows: the primary hold at 95°C for 4 min, 30 cycles of 98°C10 sec, 55°C 15 sec and 72°C 45 sec. Lanes were 100 bp ladder marker (1), T-616substrain 2 (2), T-616 substrain 1 (3), the original seed stock of T-616 (4) and 1 kbpladder marker (5). Expected sizes are 344 bps for T-616 substrain 2 and 2,058 bps forT-616 substrain 1.

Mentions: Two large deletions were observed in T-616 substrain 2 and T-529. A 1,714 bps deletion isfound in T-616 substrain 2, corresponding to nucleotides 128,715 to 130,428 in T-616 substrain1 (Fig. 2AFig. 2.


Full genome sequences of zebra-borne equine herpesvirus type 1 isolated from zebra, onager and Thomson's gazelle.

Guo X, Izume S, Okada A, Ohya K, Kimura T, Fukushi H - J. Vet. Med. Sci. (2014)

(A) Nucleotide deletion from the tail part of ORF70 to the head part of ORF71. Thearrow in black indicates the original ORF70. The other arrow in dark grey indicates thetruncated tail part of ORF70 caused by deletion. (B) Amino acid sequence alignments ofthe tail part of ORF70 in Substrains 1 and 2. The amino acid sequences with underlineindicate the corresponding sequences shown in the panel A. The amino acid sequence initalic grey indicates the truncated tail of ORF70 caused by the deletion. The asterisks(*) indicate the placement of stop codon in the original nucleotide sequence. (C) PCRresults to confirm the presence of 2 viruses in T-616. PCR primers used were as follows:Zebra_71-F 5′-ccaacgtaccatcaagtgcggta-3′ and Zebra_71-R 5′-cgctggtactctcgtaggttgac-3′.PCR was examined by using PrimeSTAR Max Premix (TaKaRa Bio, Otsu, Japan) withamplification program as follows: the primary hold at 95°C for 4 min, 30 cycles of 98°C10 sec, 55°C 15 sec and 72°C 45 sec. Lanes were 100 bp ladder marker (1), T-616substrain 2 (2), T-616 substrain 1 (3), the original seed stock of T-616 (4) and 1 kbpladder marker (5). Expected sizes are 344 bps for T-616 substrain 2 and 2,058 bps forT-616 substrain 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4197165&req=5

fig_002: (A) Nucleotide deletion from the tail part of ORF70 to the head part of ORF71. Thearrow in black indicates the original ORF70. The other arrow in dark grey indicates thetruncated tail part of ORF70 caused by deletion. (B) Amino acid sequence alignments ofthe tail part of ORF70 in Substrains 1 and 2. The amino acid sequences with underlineindicate the corresponding sequences shown in the panel A. The amino acid sequence initalic grey indicates the truncated tail of ORF70 caused by the deletion. The asterisks(*) indicate the placement of stop codon in the original nucleotide sequence. (C) PCRresults to confirm the presence of 2 viruses in T-616. PCR primers used were as follows:Zebra_71-F 5′-ccaacgtaccatcaagtgcggta-3′ and Zebra_71-R 5′-cgctggtactctcgtaggttgac-3′.PCR was examined by using PrimeSTAR Max Premix (TaKaRa Bio, Otsu, Japan) withamplification program as follows: the primary hold at 95°C for 4 min, 30 cycles of 98°C10 sec, 55°C 15 sec and 72°C 45 sec. Lanes were 100 bp ladder marker (1), T-616substrain 2 (2), T-616 substrain 1 (3), the original seed stock of T-616 (4) and 1 kbpladder marker (5). Expected sizes are 344 bps for T-616 substrain 2 and 2,058 bps forT-616 substrain 1.
Mentions: Two large deletions were observed in T-616 substrain 2 and T-529. A 1,714 bps deletion isfound in T-616 substrain 2, corresponding to nucleotides 128,715 to 130,428 in T-616 substrain1 (Fig. 2AFig. 2.

Bottom Line: The full genome sequences of the 3 strains were determined.Sequence data indicated that the EHV-1 isolated from a polar bear in Germany is one of the zebra-borne EHV-1 and not a recombinant virus.These results indicated that zebra-borne EHV-1 is a subtype of EHV-1.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.

ABSTRACT
A strain of equine herpesvirus type 1 (EHV-1) was isolated from zebra. This strain, called "zebra-borne EHV-1", was also isolated from an onager and a gazelle in zoological gardens in U.S.A. The full genome sequences of the 3 strains were determined. They shared 99% identities with each other, while they shared 98% and 95% identities with the horse derived EHV-1 and equine herpesvirus type 9, respectively. Sequence data indicated that the EHV-1 isolated from a polar bear in Germany is one of the zebra-borne EHV-1 and not a recombinant virus. These results indicated that zebra-borne EHV-1 is a subtype of EHV-1.

Show MeSH
Related in: MedlinePlus