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Pathological and immunohistochemical findings of natural highly pathogenic avian influenza infection in tufted ducks during 2010-2011 outbreaks in Japan.

Abdo W, Haridy M, Katou Y, Goto M, Mizoguchi T, Sakoda Y, Sakai H, Yanai T - J. Vet. Med. Sci. (2014)

Bottom Line: Tufted ducks were found dead in an urban area of Toyota City, Koriyama, Fukushima Prefecture.HPAIV antigen was detected in the malacic areas, neuronal, glial and ependymal cells, pulmonary capillary endothelial cells and epithelium of pulmonary bronchioles, necrotic pancreatic acini and degenerated cardiac myocytes.The HPAIV isolate was genetically classified into clade 2.3.2.1 group A.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathogenetic Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.

ABSTRACT
In the winter of 2010-2011, an outbreak of highly pathogenic avian influenza virus (HPAIV) infection occurred in wild and domestic birds in Japan. Tufted ducks were found dead in an urban area of Toyota City, Koriyama, Fukushima Prefecture. Two tufted ducks were examined histopathologically, immunohistochemically and molecularly. Gross findings included marked dark-red clotted blood in the pectoral muscles and multifocal hemorrhages on the serous membranes. Microscopically, non-suppurative meningoencephalitis, multifocal to coalescing pancreatic necrosis and severe pulmonary congestion were observed. HPAIV antigen was detected in the malacic areas, neuronal, glial and ependymal cells, pulmonary capillary endothelial cells and epithelium of pulmonary bronchioles, necrotic pancreatic acini and degenerated cardiac myocytes. The HPAIV isolate was genetically classified into clade 2.3.2.1 group A. The broad distribution of virus antigen in brain and pulmonary tissues associated with HPAIV spontaneous infection in tufted ducks might be useful in understanding its pathogenesis in nature.

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Related in: MedlinePlus

Immunohistochemical detection of avian influenza antigen in tufted ducks in (a)subcortical multifocal liquefactive necrosis and (b) ependymal, degenerated neurons andglial cells of the brain, (c) the pulmonary endothelium of the alveolar capillary, (d)epithelial cells of the parabronchus and atrium, (e) pancreatic acini and (f) focaldegenerated cardiac myocytes as well as in (g) lymphocytic infiltration in laminapropria of the intestine in positive control chicken, but not in (h) brain of negativecontrol chicken (ABC immunoperoxidase technique, counterstained with Mayer’shematoxylin).
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fig_002: Immunohistochemical detection of avian influenza antigen in tufted ducks in (a)subcortical multifocal liquefactive necrosis and (b) ependymal, degenerated neurons andglial cells of the brain, (c) the pulmonary endothelium of the alveolar capillary, (d)epithelial cells of the parabronchus and atrium, (e) pancreatic acini and (f) focaldegenerated cardiac myocytes as well as in (g) lymphocytic infiltration in laminapropria of the intestine in positive control chicken, but not in (h) brain of negativecontrol chicken (ABC immunoperoxidase technique, counterstained with Mayer’shematoxylin).

Mentions: Immunohistochemically, the avian influenza antigen was pantropic in the 2 birds. The antigenwas detected in the brain, pancreas, liver, spleen, lungs and heart. There was a strongcorrelation between the demonstration of viral antigen and the microscopic lesions. Strongviral antigen staining was demonstrated throughout the mulifocal liquefactive necrotic areasof the brain (Fig. 2aFig. 2.


Pathological and immunohistochemical findings of natural highly pathogenic avian influenza infection in tufted ducks during 2010-2011 outbreaks in Japan.

Abdo W, Haridy M, Katou Y, Goto M, Mizoguchi T, Sakoda Y, Sakai H, Yanai T - J. Vet. Med. Sci. (2014)

Immunohistochemical detection of avian influenza antigen in tufted ducks in (a)subcortical multifocal liquefactive necrosis and (b) ependymal, degenerated neurons andglial cells of the brain, (c) the pulmonary endothelium of the alveolar capillary, (d)epithelial cells of the parabronchus and atrium, (e) pancreatic acini and (f) focaldegenerated cardiac myocytes as well as in (g) lymphocytic infiltration in laminapropria of the intestine in positive control chicken, but not in (h) brain of negativecontrol chicken (ABC immunoperoxidase technique, counterstained with Mayer’shematoxylin).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4197160&req=5

fig_002: Immunohistochemical detection of avian influenza antigen in tufted ducks in (a)subcortical multifocal liquefactive necrosis and (b) ependymal, degenerated neurons andglial cells of the brain, (c) the pulmonary endothelium of the alveolar capillary, (d)epithelial cells of the parabronchus and atrium, (e) pancreatic acini and (f) focaldegenerated cardiac myocytes as well as in (g) lymphocytic infiltration in laminapropria of the intestine in positive control chicken, but not in (h) brain of negativecontrol chicken (ABC immunoperoxidase technique, counterstained with Mayer’shematoxylin).
Mentions: Immunohistochemically, the avian influenza antigen was pantropic in the 2 birds. The antigenwas detected in the brain, pancreas, liver, spleen, lungs and heart. There was a strongcorrelation between the demonstration of viral antigen and the microscopic lesions. Strongviral antigen staining was demonstrated throughout the mulifocal liquefactive necrotic areasof the brain (Fig. 2aFig. 2.

Bottom Line: Tufted ducks were found dead in an urban area of Toyota City, Koriyama, Fukushima Prefecture.HPAIV antigen was detected in the malacic areas, neuronal, glial and ependymal cells, pulmonary capillary endothelial cells and epithelium of pulmonary bronchioles, necrotic pancreatic acini and degenerated cardiac myocytes.The HPAIV isolate was genetically classified into clade 2.3.2.1 group A.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathogenetic Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.

ABSTRACT
In the winter of 2010-2011, an outbreak of highly pathogenic avian influenza virus (HPAIV) infection occurred in wild and domestic birds in Japan. Tufted ducks were found dead in an urban area of Toyota City, Koriyama, Fukushima Prefecture. Two tufted ducks were examined histopathologically, immunohistochemically and molecularly. Gross findings included marked dark-red clotted blood in the pectoral muscles and multifocal hemorrhages on the serous membranes. Microscopically, non-suppurative meningoencephalitis, multifocal to coalescing pancreatic necrosis and severe pulmonary congestion were observed. HPAIV antigen was detected in the malacic areas, neuronal, glial and ependymal cells, pulmonary capillary endothelial cells and epithelium of pulmonary bronchioles, necrotic pancreatic acini and degenerated cardiac myocytes. The HPAIV isolate was genetically classified into clade 2.3.2.1 group A. The broad distribution of virus antigen in brain and pulmonary tissues associated with HPAIV spontaneous infection in tufted ducks might be useful in understanding its pathogenesis in nature.

Show MeSH
Related in: MedlinePlus