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Group B betacoronavirus in rhinolophid bats, Japan.

Suzuki J, Sato R, Kobayashi T, Aoi T, Harasawa R - J. Vet. Med. Sci. (2014)

Bottom Line: We then used reverse-transcription PCR to look for the coronavirus RNA-dependent RNA polymerase gene in fecal samples collected from 27 little Japanese horseshoe bats and found eight were provisionally positive.We had a success in the nucleotide sequencing of six of the eight positive samples and compared them with those of authentic coronaviruses.Pathogenic trait of these bat coronaviruses remained unexplored.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan.

ABSTRACT
We report group B Betacoronavirus infection in little Japanese horseshoe bats in Iwate prefecture. We then used reverse-transcription PCR to look for the coronavirus RNA-dependent RNA polymerase gene in fecal samples collected from 27 little Japanese horseshoe bats and found eight were provisionally positive. We had a success in the nucleotide sequencing of six of the eight positive samples and compared them with those of authentic coronaviruses. We found that these six samples were positive in coronavirus infection, and they belonged to the group B Betacornavirus by phylogenetic analysis. Virus isolation using the Vero cell culture was unsuccessful. Pathogenic trait of these bat coronaviruses remained unexplored.

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Related in: MedlinePlus

A phylogenetic analysis of RdRp gene sequences among theBetacoronavirus strains (accession numbers are given in brackets).Genetic distances were computed with CLUSTAL W. Four groups (A through D) in the genusBetacoronavirus are indicated. Bat Betacoronavirusstrains Is39, Is58, Is63, Is66, Is68 and Og44 detected in Japan were included in thegroup B betacoronavirus, along with the SARS-related CoV strains. Numbers in therelevant branches refer to the values of bootstrap probability of 1,000 replications.The scale bar indicates the number of nucleotide substitutions per site.
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fig_001: A phylogenetic analysis of RdRp gene sequences among theBetacoronavirus strains (accession numbers are given in brackets).Genetic distances were computed with CLUSTAL W. Four groups (A through D) in the genusBetacoronavirus are indicated. Bat Betacoronavirusstrains Is39, Is58, Is63, Is66, Is68 and Og44 detected in Japan were included in thegroup B betacoronavirus, along with the SARS-related CoV strains. Numbers in therelevant branches refer to the values of bootstrap probability of 1,000 replications.The scale bar indicates the number of nucleotide substitutions per site.

Mentions: We had a success in the nucleotide sequencing of six of the eight positive samples and havedeposited partial nucleotide sequences of the RdRp gene to the DNA databasesunder the accession numbers AB889995 to AB890000. The 2 other samples unsuccessful innucleotide sequencing were considered to be false-positive in PCR. The six sequences (426 nt)showed more than 98% nt identity to each other. Comparison between the six CoVs from littleJapanese horseshoe bats and those from DNA databases revealed that the nucleotide sequenceshad the highest identity (about 91% nt) to bat SARS CoV strain Rp3 (GenBank accession no.DQ071615), which was isolated from fecal swabs of Pearson’s horseshoe bats (R.pearsonii) in Guangxi Province, China in 2005 [4]. Nucleotide sequences aligned using Clustal W [10] were analyzed by the neighbor-joining method [7]. The phylogenetic tree indicated that the six CoV sequences detected in littleJapanese horseshoe bats have a monophyletic relationship to the SARS CoV in the same lineageof the group B betacoronavirus (Fig. 1Fig. 1.


Group B betacoronavirus in rhinolophid bats, Japan.

Suzuki J, Sato R, Kobayashi T, Aoi T, Harasawa R - J. Vet. Med. Sci. (2014)

A phylogenetic analysis of RdRp gene sequences among theBetacoronavirus strains (accession numbers are given in brackets).Genetic distances were computed with CLUSTAL W. Four groups (A through D) in the genusBetacoronavirus are indicated. Bat Betacoronavirusstrains Is39, Is58, Is63, Is66, Is68 and Og44 detected in Japan were included in thegroup B betacoronavirus, along with the SARS-related CoV strains. Numbers in therelevant branches refer to the values of bootstrap probability of 1,000 replications.The scale bar indicates the number of nucleotide substitutions per site.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4197156&req=5

fig_001: A phylogenetic analysis of RdRp gene sequences among theBetacoronavirus strains (accession numbers are given in brackets).Genetic distances were computed with CLUSTAL W. Four groups (A through D) in the genusBetacoronavirus are indicated. Bat Betacoronavirusstrains Is39, Is58, Is63, Is66, Is68 and Og44 detected in Japan were included in thegroup B betacoronavirus, along with the SARS-related CoV strains. Numbers in therelevant branches refer to the values of bootstrap probability of 1,000 replications.The scale bar indicates the number of nucleotide substitutions per site.
Mentions: We had a success in the nucleotide sequencing of six of the eight positive samples and havedeposited partial nucleotide sequences of the RdRp gene to the DNA databasesunder the accession numbers AB889995 to AB890000. The 2 other samples unsuccessful innucleotide sequencing were considered to be false-positive in PCR. The six sequences (426 nt)showed more than 98% nt identity to each other. Comparison between the six CoVs from littleJapanese horseshoe bats and those from DNA databases revealed that the nucleotide sequenceshad the highest identity (about 91% nt) to bat SARS CoV strain Rp3 (GenBank accession no.DQ071615), which was isolated from fecal swabs of Pearson’s horseshoe bats (R.pearsonii) in Guangxi Province, China in 2005 [4]. Nucleotide sequences aligned using Clustal W [10] were analyzed by the neighbor-joining method [7]. The phylogenetic tree indicated that the six CoV sequences detected in littleJapanese horseshoe bats have a monophyletic relationship to the SARS CoV in the same lineageof the group B betacoronavirus (Fig. 1Fig. 1.

Bottom Line: We then used reverse-transcription PCR to look for the coronavirus RNA-dependent RNA polymerase gene in fecal samples collected from 27 little Japanese horseshoe bats and found eight were provisionally positive.We had a success in the nucleotide sequencing of six of the eight positive samples and compared them with those of authentic coronaviruses.Pathogenic trait of these bat coronaviruses remained unexplored.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan.

ABSTRACT
We report group B Betacoronavirus infection in little Japanese horseshoe bats in Iwate prefecture. We then used reverse-transcription PCR to look for the coronavirus RNA-dependent RNA polymerase gene in fecal samples collected from 27 little Japanese horseshoe bats and found eight were provisionally positive. We had a success in the nucleotide sequencing of six of the eight positive samples and compared them with those of authentic coronaviruses. We found that these six samples were positive in coronavirus infection, and they belonged to the group B Betacornavirus by phylogenetic analysis. Virus isolation using the Vero cell culture was unsuccessful. Pathogenic trait of these bat coronaviruses remained unexplored.

Show MeSH
Related in: MedlinePlus