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Characterization of SET/I2PP2A isoforms in dogs.

Yabe R, Fujiwara N, Mizuno T, Usui T, Ohama T, Sato K - J. Vet. Med. Sci. (2014)

Bottom Line: Here, we found that canine cells express several isoforms of the SET protein.We confirmed that SETα and β possess the ability to associate with PP2A.Our data reveal the existence of unique SET isoforms that should be taken into account in SET-targeting drug development studies in dogs.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan.

ABSTRACT
SET is an endogenous protein phosphatase 2A (PP2A) inhibitor and is associated with a poor prognosis in human leukemia. Previously, we reported increased SET protein levels in canine lymphoma cell lines and the potential therapeutic application of SET antagonists in canine lymphoma. Here, we found that canine cells express several isoforms of the SET protein. We cloned 4 isoforms of SET, named SETα, β, γ and δ. Genomic BLAST showed that the SET genes are located on chromosomes X, 7, 1 and 8, respectively. An immunofluorescent study showed nuclear localization of SETα and β, and nuclear and cytosolic localization of SETγ and δ. We confirmed that SETα and β possess the ability to associate with PP2A. Our data reveal the existence of unique SET isoforms that should be taken into account in SET-targeting drug development studies in dogs.

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Nucleotide sequence alignment of canine SET isoforms. cDNA sequences for canine SETisoforms were aligned using Clustal X2. “*”, “:” and “.” indicate “full,” “strong,”and “weak” conserved residues, respectively. The square indicates the stop codon.
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fig_002: Nucleotide sequence alignment of canine SET isoforms. cDNA sequences for canine SETisoforms were aligned using Clustal X2. “*”, “:” and “.” indicate “full,” “strong,”and “weak” conserved residues, respectively. The square indicates the stop codon.

Mentions: To determine the sequence of canine SET isoforms, we cloned the protein cDNA from theBeagle thymus, as described in the Materials and Methods. Interestingly, the same primerisolated 4 types of cDNA sequences. cDNA sequences for 4 isoforms, named α, β, γ and δ, werealigned using Clustal X2 (Fig. 2Fig. 2.


Characterization of SET/I2PP2A isoforms in dogs.

Yabe R, Fujiwara N, Mizuno T, Usui T, Ohama T, Sato K - J. Vet. Med. Sci. (2014)

Nucleotide sequence alignment of canine SET isoforms. cDNA sequences for canine SETisoforms were aligned using Clustal X2. “*”, “:” and “.” indicate “full,” “strong,”and “weak” conserved residues, respectively. The square indicates the stop codon.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4197151&req=5

fig_002: Nucleotide sequence alignment of canine SET isoforms. cDNA sequences for canine SETisoforms were aligned using Clustal X2. “*”, “:” and “.” indicate “full,” “strong,”and “weak” conserved residues, respectively. The square indicates the stop codon.
Mentions: To determine the sequence of canine SET isoforms, we cloned the protein cDNA from theBeagle thymus, as described in the Materials and Methods. Interestingly, the same primerisolated 4 types of cDNA sequences. cDNA sequences for 4 isoforms, named α, β, γ and δ, werealigned using Clustal X2 (Fig. 2Fig. 2.

Bottom Line: Here, we found that canine cells express several isoforms of the SET protein.We confirmed that SETα and β possess the ability to associate with PP2A.Our data reveal the existence of unique SET isoforms that should be taken into account in SET-targeting drug development studies in dogs.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan.

ABSTRACT
SET is an endogenous protein phosphatase 2A (PP2A) inhibitor and is associated with a poor prognosis in human leukemia. Previously, we reported increased SET protein levels in canine lymphoma cell lines and the potential therapeutic application of SET antagonists in canine lymphoma. Here, we found that canine cells express several isoforms of the SET protein. We cloned 4 isoforms of SET, named SETα, β, γ and δ. Genomic BLAST showed that the SET genes are located on chromosomes X, 7, 1 and 8, respectively. An immunofluorescent study showed nuclear localization of SETα and β, and nuclear and cytosolic localization of SETγ and δ. We confirmed that SETα and β possess the ability to associate with PP2A. Our data reveal the existence of unique SET isoforms that should be taken into account in SET-targeting drug development studies in dogs.

Show MeSH
Related in: MedlinePlus