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BraLTP1, a lipid transfer protein gene involved in epicuticular wax deposition, cell proliferation and flower development in Brassica napus.

Liu F, Xiong X, Wu L, Fu D, Hayward A, Zeng X, Cao Y, Wu Y, Li Y, Wu G - PLoS ONE (2014)

Bottom Line: Overexpression of BnaLTP1 in B. napus caused abnormal green coloration and reduced wax deposition on leaves and detailed wax analysis revealed 17-80% reduction in various major wax components, which resulted in significant water-loss relative to wild type.This was consistent with altered expression of a a number of ABC model genes related to flower development.Together, these results suggest that BraLTP1 is a new nsLTP gene involved in wax production or deposition, with additional direct or indirect effects on cell division and flower development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, China.

ABSTRACT
Plant non-specific lipid transfer proteins (nsLTPs) constitute large multigene families that possess complex physiological functions, many of which remain unclear. This study isolated and characterized the function of a lipid transfer protein gene, BraLTP1 from Brassica rapa, in the important oilseed crops Brassica napus. BraLTP1 encodes a predicted secretory protein, in the little known VI Class of nsLTP families. Overexpression of BnaLTP1 in B. napus caused abnormal green coloration and reduced wax deposition on leaves and detailed wax analysis revealed 17-80% reduction in various major wax components, which resulted in significant water-loss relative to wild type. BnaLTP1 overexpressing leaves exhibited morphological disfiguration and abaxially curled leaf edges, and leaf cross-sections revealed cell overproliferation that was correlated to increased cytokinin levels (tZ, tZR, iP, and iPR) in leaves and high expression of the cytokinin biosynthsis gene IPT3. BnaLTP1-overexpressing plants also displayed morphological disfiguration of flowers, with early-onset and elongated carpel development and outwardly curled stamen. This was consistent with altered expression of a a number of ABC model genes related to flower development. Together, these results suggest that BraLTP1 is a new nsLTP gene involved in wax production or deposition, with additional direct or indirect effects on cell division and flower development.

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Cytokinin content in BraLTP1 overexpressing leaves.Amounts of tZ, tZR, iP, and iPR were measured in leaves of 4-week-old BraLTP1-22 and wild type (WT). Data are presented as mean±SD (n = 3).
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pone-0110272-g008: Cytokinin content in BraLTP1 overexpressing leaves.Amounts of tZ, tZR, iP, and iPR were measured in leaves of 4-week-old BraLTP1-22 and wild type (WT). Data are presented as mean±SD (n = 3).

Mentions: To correlate these changes to cytokinin composition, we quantified cytokinin content, including the levels of the cytokinins isopentenyladenine (iP) and trans-zeatin (tZ), and of their riboside (iPR and tZR), As observed in BraLTP1-22 line, the BraLTP1 overexpressing leaves contained significantly higher amounts of tZ, tZR, iP, and iPR when compared with the wild type (P<0.01, Fig. 8), consistent with more cell accumulation in leaves (Fig. 7B). This indicates that active cytokinins are highly synthesized in leaves overexpressing BraLTP1.


BraLTP1, a lipid transfer protein gene involved in epicuticular wax deposition, cell proliferation and flower development in Brassica napus.

Liu F, Xiong X, Wu L, Fu D, Hayward A, Zeng X, Cao Y, Wu Y, Li Y, Wu G - PLoS ONE (2014)

Cytokinin content in BraLTP1 overexpressing leaves.Amounts of tZ, tZR, iP, and iPR were measured in leaves of 4-week-old BraLTP1-22 and wild type (WT). Data are presented as mean±SD (n = 3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4196963&req=5

pone-0110272-g008: Cytokinin content in BraLTP1 overexpressing leaves.Amounts of tZ, tZR, iP, and iPR were measured in leaves of 4-week-old BraLTP1-22 and wild type (WT). Data are presented as mean±SD (n = 3).
Mentions: To correlate these changes to cytokinin composition, we quantified cytokinin content, including the levels of the cytokinins isopentenyladenine (iP) and trans-zeatin (tZ), and of their riboside (iPR and tZR), As observed in BraLTP1-22 line, the BraLTP1 overexpressing leaves contained significantly higher amounts of tZ, tZR, iP, and iPR when compared with the wild type (P<0.01, Fig. 8), consistent with more cell accumulation in leaves (Fig. 7B). This indicates that active cytokinins are highly synthesized in leaves overexpressing BraLTP1.

Bottom Line: Overexpression of BnaLTP1 in B. napus caused abnormal green coloration and reduced wax deposition on leaves and detailed wax analysis revealed 17-80% reduction in various major wax components, which resulted in significant water-loss relative to wild type.This was consistent with altered expression of a a number of ABC model genes related to flower development.Together, these results suggest that BraLTP1 is a new nsLTP gene involved in wax production or deposition, with additional direct or indirect effects on cell division and flower development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, China.

ABSTRACT
Plant non-specific lipid transfer proteins (nsLTPs) constitute large multigene families that possess complex physiological functions, many of which remain unclear. This study isolated and characterized the function of a lipid transfer protein gene, BraLTP1 from Brassica rapa, in the important oilseed crops Brassica napus. BraLTP1 encodes a predicted secretory protein, in the little known VI Class of nsLTP families. Overexpression of BnaLTP1 in B. napus caused abnormal green coloration and reduced wax deposition on leaves and detailed wax analysis revealed 17-80% reduction in various major wax components, which resulted in significant water-loss relative to wild type. BnaLTP1 overexpressing leaves exhibited morphological disfiguration and abaxially curled leaf edges, and leaf cross-sections revealed cell overproliferation that was correlated to increased cytokinin levels (tZ, tZR, iP, and iPR) in leaves and high expression of the cytokinin biosynthsis gene IPT3. BnaLTP1-overexpressing plants also displayed morphological disfiguration of flowers, with early-onset and elongated carpel development and outwardly curled stamen. This was consistent with altered expression of a a number of ABC model genes related to flower development. Together, these results suggest that BraLTP1 is a new nsLTP gene involved in wax production or deposition, with additional direct or indirect effects on cell division and flower development.

Show MeSH