Systematic lipidomic analysis of yeast protein kinase and phosphatase mutants reveals novel insights into regulation of lipid homeostasis.
Bottom Line: Our approach successfully identified known kinases involved in lipid homeostasis and uncovered new ones.By clustering analysis, we found connections between nutrient-sensing pathways and regulation of glycerophospholipids.We also found several new candidates for the regulation of sphingolipid homeostasis, including a connection between inositol pyrophosphate metabolism and complex sphingolipid homeostasis through transcriptional regulation of AUR1 and SUR1.
Affiliation: Department of Biochemistry, University of Geneva, Geneva CH-1211, Switzerland National Centre of Competence in Research "Chemical Biology,", University of Geneva, Geneva CH-1211, Switzerland.Show MeSH
Mentions: SNF1 and TOR1 are major regulators of energy homeostasis in yeast. They are central players in glucose- and nitrogen-sensing pathways, respectively, as well as regulators of the switch between catabolism and anabolism. The signals triggered by Snf1p and Tor1p induce opposite responses (activation/repression) of similar processes (Usaite et al., 2009; Zhang et al., 2011). By comparing the changes in glycerophospholipid (GPL) profile in the two mutant strains (Figure 4A), we observed that the lipid composition also reflects this functional anticorrelation. The major differences between snf1Δ and tor1Δ mutants are in the fatty acid chain length of GPLs (Figure 2B and Supplemental Table S5).
Affiliation: Department of Biochemistry, University of Geneva, Geneva CH-1211, Switzerland National Centre of Competence in Research "Chemical Biology,", University of Geneva, Geneva CH-1211, Switzerland.