Arrestins function in cAR1 GPCR-mediated signaling and cAR1 internalization in the development of Dictyostelium discoideum.
Bottom Line: Although the G protein-coupled cAMP receptor cAR1 and ERK2 are both implicated in regulating the oscillation, the signaling circuit remains unknown.Cells lacking arrestins (adcB(-)C(-)) display cAMP oscillations during the aggregation stage that are twice as frequent as for wild- type cells.In addition, ligand-induced cAR1 internalization is compromised in adcB(-)C(-) cells, suggesting that arrestins are involved in elimination of high-affinity cAR1 receptors from cell surface after the aggregation stage of multicellular development.
Affiliation: Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.Show MeSH
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Mentions: To reveal the potential roles of these genes in the development of D. discoideum, we analyzed the developmental phenotypes of adcB−, adcC−, and adcB −C− cells on bacterial lawns (Figure 1A and Supplemental Figure S3) and on nonnutrient agar (Figure 1C). Whereas adcB− and adcC− cells displayed a wild-type-like phenotype, adcB−C− cells were unable to form a normal number of mounds in each plaque (Figure 1, A and B) and failed to aggregate by 5 h, but they eventually formed smaller aggregates at 10 h on nonnutrient agar (Figure 1C). The developmental phenotype was partially rescued by expressing either yellow fluorescent protein (YFP)–tagged AdcB or AdcC (Figure 1, A and B), indicating that either AdcB or AdcC is required for proper development of D. discoideum and that both AdcB-YFP and AdcC-YFP are functional.
Affiliation: Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.