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Telocytes in liver regeneration: possible roles.

Wang F, Song Y, Bei Y, Zhao Y, Xiao J, Yang C - J. Cell. Mol. Med. (2014)

Bottom Line: The number of TCs detected by double labelling immunofluorescence methods (CD34/PDGFR-α, CD34/PDGFR-ß and CD34/Vimentin) was significantly increased when a high level of hepatic cell proliferation rate (almost doubled) as shown by 5-ethynyl-2'-deoxyuridine (EdU) immunostaining and Western Blot of Proliferating cell nuclear antigen (PCNA) was found at 48 and 72 hrs post-PH.Meanwhile, the number of CK-19 positive-hepatic stem cells peaked at 72 hrs post-PH, co-ordinating with the same time-point, when the number of TCs was most significantly increased.Besides intercellular junctions, we may speculate a paracrine effect via ectovesicles.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology and Hepatology, Digestive Disease Institute, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.

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Detection for TCs by double labelling immunofluorescence methods (CD34/Vimentin). Detection for TCs by CD34/Vimentin double immunofluorescence labelling in liver post-PH. Confocal laser scanning microscopy: double labelling immunofluorescence shows CD34 (green) and Vimentin (red) double-positive cells (pointed with arrows). Nuclei were counterstained with DAPI (blue). Original magnification 400×; scale bar = 20 μm. *P < 0.05.
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fig04: Detection for TCs by double labelling immunofluorescence methods (CD34/Vimentin). Detection for TCs by CD34/Vimentin double immunofluorescence labelling in liver post-PH. Confocal laser scanning microscopy: double labelling immunofluorescence shows CD34 (green) and Vimentin (red) double-positive cells (pointed with arrows). Nuclei were counterstained with DAPI (blue). Original magnification 400×; scale bar = 20 μm. *P < 0.05.

Mentions: To detect TCs in mice liver, three different double labelling immunofluorescence methods (CD34/PDGFR-α, CD34/PDGFR-ß and CD34/Vimentin) were conducted. The number of CD34/PDGFR-α double-positive cells was significantly increased at 72 hrs [P = 0.012, 95% CI = (0.42, 2.57)] post-PH (Fig. 2), and significant increased number of CD34/PDGFR-ß double-positive cells was observed at 48 hrs [P = 0.006, 95% CI = (1.49, 6.12)] and 72 hrs [P = 0.001, 95% CI = (4.46, 11.53)] post-PH (Fig. 3), while the increase in CD34/Vimentin double-positive cells was observed at 48 hrs [P = 2.36 × 10−16, 95% CI = (25.38, 35.63)], 72 hrs [P = 1.36 × 10−22, 95% CI = (45.16, 54.84)], 96 hrs [P = 1.53 × 10−16, 95% CI = (24.41, 34.09)] and 120 hrs [P = 1.87 × 10−9, 95% CI = (9.91, 19.59); Fig. 4], corresponding to the proliferative peak time-point of liver regeneration post-PH.


Telocytes in liver regeneration: possible roles.

Wang F, Song Y, Bei Y, Zhao Y, Xiao J, Yang C - J. Cell. Mol. Med. (2014)

Detection for TCs by double labelling immunofluorescence methods (CD34/Vimentin). Detection for TCs by CD34/Vimentin double immunofluorescence labelling in liver post-PH. Confocal laser scanning microscopy: double labelling immunofluorescence shows CD34 (green) and Vimentin (red) double-positive cells (pointed with arrows). Nuclei were counterstained with DAPI (blue). Original magnification 400×; scale bar = 20 μm. *P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4196648&req=5

fig04: Detection for TCs by double labelling immunofluorescence methods (CD34/Vimentin). Detection for TCs by CD34/Vimentin double immunofluorescence labelling in liver post-PH. Confocal laser scanning microscopy: double labelling immunofluorescence shows CD34 (green) and Vimentin (red) double-positive cells (pointed with arrows). Nuclei were counterstained with DAPI (blue). Original magnification 400×; scale bar = 20 μm. *P < 0.05.
Mentions: To detect TCs in mice liver, three different double labelling immunofluorescence methods (CD34/PDGFR-α, CD34/PDGFR-ß and CD34/Vimentin) were conducted. The number of CD34/PDGFR-α double-positive cells was significantly increased at 72 hrs [P = 0.012, 95% CI = (0.42, 2.57)] post-PH (Fig. 2), and significant increased number of CD34/PDGFR-ß double-positive cells was observed at 48 hrs [P = 0.006, 95% CI = (1.49, 6.12)] and 72 hrs [P = 0.001, 95% CI = (4.46, 11.53)] post-PH (Fig. 3), while the increase in CD34/Vimentin double-positive cells was observed at 48 hrs [P = 2.36 × 10−16, 95% CI = (25.38, 35.63)], 72 hrs [P = 1.36 × 10−22, 95% CI = (45.16, 54.84)], 96 hrs [P = 1.53 × 10−16, 95% CI = (24.41, 34.09)] and 120 hrs [P = 1.87 × 10−9, 95% CI = (9.91, 19.59); Fig. 4], corresponding to the proliferative peak time-point of liver regeneration post-PH.

Bottom Line: The number of TCs detected by double labelling immunofluorescence methods (CD34/PDGFR-α, CD34/PDGFR-ß and CD34/Vimentin) was significantly increased when a high level of hepatic cell proliferation rate (almost doubled) as shown by 5-ethynyl-2'-deoxyuridine (EdU) immunostaining and Western Blot of Proliferating cell nuclear antigen (PCNA) was found at 48 and 72 hrs post-PH.Meanwhile, the number of CK-19 positive-hepatic stem cells peaked at 72 hrs post-PH, co-ordinating with the same time-point, when the number of TCs was most significantly increased.Besides intercellular junctions, we may speculate a paracrine effect via ectovesicles.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology and Hepatology, Digestive Disease Institute, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.

Show MeSH
Related in: MedlinePlus