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Topiramate ameliorates abdominal aorta cross-clamping induced liver injury in rats.

Cure E, Cure MC, Tumkaya L, Kalkan Y, Aydin I, Kirbas A, Yilmaz A, Yuce S, Gokce MF - Saudi J Gastroenterol (2014 Sep-Oct)

Bottom Line: The antioxidant and tumor necrosis factor-alpha (TNF-α) suppression effects of topiramate (TPM) have been reported in several studies.The I/R group's TNF-α and interleukin-6 (IL-6) levels were significantly higher than those of the control (P = 0.010; P = 0.002) and I/R + TPM groups (P = 0.010; P = 0.002, respectively).TPM pretreatment may have preventive effects on liver injury via I/R during intra-abdominal surgery.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Recep Tayyip Erdogan University, School of Medicine, Rize, Turkey.

ABSTRACT

Background and aim: Ischemia/reperfusion (I/R) injury in the liver occurs after a prolonged period of ischemia followed by restoration of hepatic blood perfusion. During the surgery of abdominal aorta, I/R injury causes damage to lower extremities and many organs, especially liver. The antioxidant and tumor necrosis factor-alpha (TNF-α) suppression effects of topiramate (TPM) have been reported in several studies. We evaluated the potential protective effect of TPM on cellular damage in liver tissue during I/R injury.

Materials and methods: Thirty male Wistar albino rats were divided into three groups: Control, I/R, and I/R plus TPM (I/R + TPM) groups. Laparotomy without I/R injury was performed in the control group. After laparotomy, cross-ligation of infrarenal abdominal aorta was applied for 2 h in I/R groups that was followed by 2 h of reperfusion. TPM (100 mg/kg/day) was orally administrated to the animals in the I/R + TPM group for seven consecutive days before I/R procedure.

Results: The I/R group's TNF-α and interleukin-6 (IL-6) levels were significantly higher than those of the control (P = 0.010; P = 0.002) and I/R + TPM groups (P = 0.010; P = 0.002, respectively). Asymmetric dimethyl arginine (ADMA) levels of I/R group were higher than the control (P = 0.015) and I/R + TPM groups. I/R caused serious histopathological damage to liver tissue; however, TPM led to very low histopathological changes.

Conclusion: Our data demonstrated that TPM treatment prominently decreases the severity of liver I/R injury. TPM pretreatment may have preventive effects on liver injury via I/R during intra-abdominal surgery.

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Histopathologic evaluation of kidney tissue stained by immunoperoxidase method by light microscopy. (a) Control group, (b) I/R applied group, (c) I/R+TPM applied group. de, Degeneration; v, vacuolization; immunoperoxidase–stained antiliver arginase antibody
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Figure 3: Histopathologic evaluation of kidney tissue stained by immunoperoxidase method by light microscopy. (a) Control group, (b) I/R applied group, (c) I/R+TPM applied group. de, Degeneration; v, vacuolization; immunoperoxidase–stained antiliver arginase antibody

Mentions: The histopathological examination after H and E stain method in ischemic reperfusion and TPM applied group revealed lower tissue and cellular deformity than ischemic reperfusion group [Table 1 and Figure 1c]. The intensive sinusoidal dilatations of I/R group were lower in the control group. However, the two groups have similar elongated sinusoidal structures. When compared with the I/R group, Kupffer cells settling the sinusoidal walls were observed to be more flat and stained deep basophilic. Vacuolization of the hepatocytes were lower in this group. However, they were observed around the portal region [Figure 1c]. The immunopositivity of CA-II in the hepatic tissues of the control and application groups was more intensively positive in sinusoidal walls and in the surroundings of the central vein. Although there was a decrease in immunoreactivity in progress to portal regions, it had a continuous course. Although immunoreactivity of the control group showed homogeneous distribution, it showed a decrease in the homogeneity and positivity when moving away from the central vein in the application group [Figure 2]. The immunopositivity of antiliver arginase in the hepatic tissues of the control group has low immunoreactivity in the surroundings of central vein. Although there was a decrease in immunoreactivity in progress to portal regions, it was lower in the periphery of the portal region. There was an extreme decrease in the antiliver arginase immunoreactivity [20% (+)] in necrotic and degenerative hepatocytes [Figure 3]. Histopathological results are shown in table 2.


Topiramate ameliorates abdominal aorta cross-clamping induced liver injury in rats.

Cure E, Cure MC, Tumkaya L, Kalkan Y, Aydin I, Kirbas A, Yilmaz A, Yuce S, Gokce MF - Saudi J Gastroenterol (2014 Sep-Oct)

Histopathologic evaluation of kidney tissue stained by immunoperoxidase method by light microscopy. (a) Control group, (b) I/R applied group, (c) I/R+TPM applied group. de, Degeneration; v, vacuolization; immunoperoxidase–stained antiliver arginase antibody
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4196345&req=5

Figure 3: Histopathologic evaluation of kidney tissue stained by immunoperoxidase method by light microscopy. (a) Control group, (b) I/R applied group, (c) I/R+TPM applied group. de, Degeneration; v, vacuolization; immunoperoxidase–stained antiliver arginase antibody
Mentions: The histopathological examination after H and E stain method in ischemic reperfusion and TPM applied group revealed lower tissue and cellular deformity than ischemic reperfusion group [Table 1 and Figure 1c]. The intensive sinusoidal dilatations of I/R group were lower in the control group. However, the two groups have similar elongated sinusoidal structures. When compared with the I/R group, Kupffer cells settling the sinusoidal walls were observed to be more flat and stained deep basophilic. Vacuolization of the hepatocytes were lower in this group. However, they were observed around the portal region [Figure 1c]. The immunopositivity of CA-II in the hepatic tissues of the control and application groups was more intensively positive in sinusoidal walls and in the surroundings of the central vein. Although there was a decrease in immunoreactivity in progress to portal regions, it had a continuous course. Although immunoreactivity of the control group showed homogeneous distribution, it showed a decrease in the homogeneity and positivity when moving away from the central vein in the application group [Figure 2]. The immunopositivity of antiliver arginase in the hepatic tissues of the control group has low immunoreactivity in the surroundings of central vein. Although there was a decrease in immunoreactivity in progress to portal regions, it was lower in the periphery of the portal region. There was an extreme decrease in the antiliver arginase immunoreactivity [20% (+)] in necrotic and degenerative hepatocytes [Figure 3]. Histopathological results are shown in table 2.

Bottom Line: The antioxidant and tumor necrosis factor-alpha (TNF-α) suppression effects of topiramate (TPM) have been reported in several studies.The I/R group's TNF-α and interleukin-6 (IL-6) levels were significantly higher than those of the control (P = 0.010; P = 0.002) and I/R + TPM groups (P = 0.010; P = 0.002, respectively).TPM pretreatment may have preventive effects on liver injury via I/R during intra-abdominal surgery.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Recep Tayyip Erdogan University, School of Medicine, Rize, Turkey.

ABSTRACT

Background and aim: Ischemia/reperfusion (I/R) injury in the liver occurs after a prolonged period of ischemia followed by restoration of hepatic blood perfusion. During the surgery of abdominal aorta, I/R injury causes damage to lower extremities and many organs, especially liver. The antioxidant and tumor necrosis factor-alpha (TNF-α) suppression effects of topiramate (TPM) have been reported in several studies. We evaluated the potential protective effect of TPM on cellular damage in liver tissue during I/R injury.

Materials and methods: Thirty male Wistar albino rats were divided into three groups: Control, I/R, and I/R plus TPM (I/R + TPM) groups. Laparotomy without I/R injury was performed in the control group. After laparotomy, cross-ligation of infrarenal abdominal aorta was applied for 2 h in I/R groups that was followed by 2 h of reperfusion. TPM (100 mg/kg/day) was orally administrated to the animals in the I/R + TPM group for seven consecutive days before I/R procedure.

Results: The I/R group's TNF-α and interleukin-6 (IL-6) levels were significantly higher than those of the control (P = 0.010; P = 0.002) and I/R + TPM groups (P = 0.010; P = 0.002, respectively). Asymmetric dimethyl arginine (ADMA) levels of I/R group were higher than the control (P = 0.015) and I/R + TPM groups. I/R caused serious histopathological damage to liver tissue; however, TPM led to very low histopathological changes.

Conclusion: Our data demonstrated that TPM treatment prominently decreases the severity of liver I/R injury. TPM pretreatment may have preventive effects on liver injury via I/R during intra-abdominal surgery.

Show MeSH
Related in: MedlinePlus