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Antimicrobial and anti-adherence activity of various combinations of coffee-chicory solutions on Streptococcus mutans: An in-vitro study.

Sharma R, Reddy VK, Prashant G, Ojha V, Kumar NP - J Oral Maxillofac Pathol (2014)

Bottom Line: Pure chicory had shown significantly less bacterial count compared to all other groups.Regarding anti-adherence effect, group I-IV had shown significantly less adherence of bacteria to glass surface.Chicory exerted antibacterial effect against S. mutans while coffee reduced significantly the adherence of S. mutans to the glass surface.

View Article: PubMed Central - PubMed

Affiliation: Department of Public Health Dentistry, Babu Banarasi Das College of Dental Sciences, Lucknow, Uttar Pradesh, India.

ABSTRACT

Context: Several studies have demonstrated the activity of natural plants on the dental biofilm and caries development. But few studies on the antimicrobial activity of coffee-based solutions were found in the literature. Further there was no study available to check the antimicrobial effect of coffee solutions with different percentages of chicory in it.

Aims: To evaluate the antimicrobial activity of different combinations of coffee-chicory solutions and their anti-adherence effect on Streptococcus mutans to glass surface.

Materials and methods: Test solutions were prepared. For antimicrobial activity testing, tubes containing test solution and culture medium were inoculated with a suspension of S. mutans followed by plating on Brain Heart Infusion (BHI) agar. S. mutans adherence to glass in presence of the different test solutions was also tested. The number of adhered bacteria (CFU/mL) was determined by plating method.

Statistical analysis: Statistical significance was measured using one way ANOVA followed by Tukey's post hoc test. P value < 0.05 was considered statistically significant.

Results: Pure chicory had shown significantly less bacterial count compared to all other groups. Groups IV and V had shown significant reduction in bacterial counts over the period of 4 hrs. Regarding anti-adherence effect, group I-IV had shown significantly less adherence of bacteria to glass surface.

Conclusions: Chicory exerted antibacterial effect against S. mutans while coffee reduced significantly the adherence of S. mutans to the glass surface.

No MeSH data available.


Related in: MedlinePlus

Mean of log CFU/ml for the experimental groups after 1 min, 1 hr, 2 hrs and 4 hrs contact of Streptococcus mutans with test solutions
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Figure 4: Mean of log CFU/ml for the experimental groups after 1 min, 1 hr, 2 hrs and 4 hrs contact of Streptococcus mutans with test solutions

Mentions: Figure 4 show, that after first and second hour, similar results were observed as observed after 1 minute. After 4 hours, all the results obtained were similar to the results mentioned above except the results of group IV. It showed significantly less colony counts as compared to 1st three groups.


Antimicrobial and anti-adherence activity of various combinations of coffee-chicory solutions on Streptococcus mutans: An in-vitro study.

Sharma R, Reddy VK, Prashant G, Ojha V, Kumar NP - J Oral Maxillofac Pathol (2014)

Mean of log CFU/ml for the experimental groups after 1 min, 1 hr, 2 hrs and 4 hrs contact of Streptococcus mutans with test solutions
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4196287&req=5

Figure 4: Mean of log CFU/ml for the experimental groups after 1 min, 1 hr, 2 hrs and 4 hrs contact of Streptococcus mutans with test solutions
Mentions: Figure 4 show, that after first and second hour, similar results were observed as observed after 1 minute. After 4 hours, all the results obtained were similar to the results mentioned above except the results of group IV. It showed significantly less colony counts as compared to 1st three groups.

Bottom Line: Pure chicory had shown significantly less bacterial count compared to all other groups.Regarding anti-adherence effect, group I-IV had shown significantly less adherence of bacteria to glass surface.Chicory exerted antibacterial effect against S. mutans while coffee reduced significantly the adherence of S. mutans to the glass surface.

View Article: PubMed Central - PubMed

Affiliation: Department of Public Health Dentistry, Babu Banarasi Das College of Dental Sciences, Lucknow, Uttar Pradesh, India.

ABSTRACT

Context: Several studies have demonstrated the activity of natural plants on the dental biofilm and caries development. But few studies on the antimicrobial activity of coffee-based solutions were found in the literature. Further there was no study available to check the antimicrobial effect of coffee solutions with different percentages of chicory in it.

Aims: To evaluate the antimicrobial activity of different combinations of coffee-chicory solutions and their anti-adherence effect on Streptococcus mutans to glass surface.

Materials and methods: Test solutions were prepared. For antimicrobial activity testing, tubes containing test solution and culture medium were inoculated with a suspension of S. mutans followed by plating on Brain Heart Infusion (BHI) agar. S. mutans adherence to glass in presence of the different test solutions was also tested. The number of adhered bacteria (CFU/mL) was determined by plating method.

Statistical analysis: Statistical significance was measured using one way ANOVA followed by Tukey's post hoc test. P value < 0.05 was considered statistically significant.

Results: Pure chicory had shown significantly less bacterial count compared to all other groups. Groups IV and V had shown significant reduction in bacterial counts over the period of 4 hrs. Regarding anti-adherence effect, group I-IV had shown significantly less adherence of bacteria to glass surface.

Conclusions: Chicory exerted antibacterial effect against S. mutans while coffee reduced significantly the adherence of S. mutans to the glass surface.

No MeSH data available.


Related in: MedlinePlus