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Antimicrobial and anti-adherence activity of various combinations of coffee-chicory solutions on Streptococcus mutans: An in-vitro study.

Sharma R, Reddy VK, Prashant G, Ojha V, Kumar NP - J Oral Maxillofac Pathol (2014)

Bottom Line: Pure chicory had shown significantly less bacterial count compared to all other groups.Groups IV and V had shown significant reduction in bacterial counts over the period of 4 hrs.Chicory exerted antibacterial effect against S. mutans while coffee reduced significantly the adherence of S. mutans to the glass surface.

View Article: PubMed Central - PubMed

Affiliation: Department of Public Health Dentistry, Babu Banarasi Das College of Dental Sciences, Lucknow, Uttar Pradesh, India.

ABSTRACT

Context: Several studies have demonstrated the activity of natural plants on the dental biofilm and caries development. But few studies on the antimicrobial activity of coffee-based solutions were found in the literature. Further there was no study available to check the antimicrobial effect of coffee solutions with different percentages of chicory in it.

Aims: To evaluate the antimicrobial activity of different combinations of coffee-chicory solutions and their anti-adherence effect on Streptococcus mutans to glass surface.

Materials and methods: Test solutions were prepared. For antimicrobial activity testing, tubes containing test solution and culture medium were inoculated with a suspension of S. mutans followed by plating on Brain Heart Infusion (BHI) agar. S. mutans adherence to glass in presence of the different test solutions was also tested. The number of adhered bacteria (CFU/mL) was determined by plating method.

Statistical analysis: Statistical significance was measured using one way ANOVA followed by Tukey's post hoc test. P value < 0.05 was considered statistically significant.

Results: Pure chicory had shown significantly less bacterial count compared to all other groups. Groups IV and V had shown significant reduction in bacterial counts over the period of 4 hrs. Regarding anti-adherence effect, group I-IV had shown significantly less adherence of bacteria to glass surface.

Conclusions: Chicory exerted antibacterial effect against S. mutans while coffee reduced significantly the adherence of S. mutans to the glass surface.

No MeSH data available.


Related in: MedlinePlus

BHI agar plates showing bacterial colonies
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Figure 2: BHI agar plates showing bacterial colonies

Mentions: A standardized Streptococcus mutans (ATCC 25175) suspension was obtained by pipetting one milliliter of the bacterial culture into the sterile test tube containing 1 ml of broth. Then normal saline was added gradually to it so as to compare the turbidity to that of 0.5 Mac Farland Standard that corresponded to approximately 1.5 × 108 cells/ml.[5] After sterilization of the test solutions, each tube was inoculated with 1 ml of the bacterial suspension and incubated at 37°C for 1 minute, 1 hour, 2 hours and 4 hours [Figure 1]. After the incubation period, 0.1 ml of each tube's content was plated in duplicate on BHI agar. Plates were incubated for 48 hours at 37°C. After this period, the number of colonies [Figure 2] were counted and the value of logarithm of colony forming units per milliliter was calculated (log CFU/ml).[3]


Antimicrobial and anti-adherence activity of various combinations of coffee-chicory solutions on Streptococcus mutans: An in-vitro study.

Sharma R, Reddy VK, Prashant G, Ojha V, Kumar NP - J Oral Maxillofac Pathol (2014)

BHI agar plates showing bacterial colonies
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4196287&req=5

Figure 2: BHI agar plates showing bacterial colonies
Mentions: A standardized Streptococcus mutans (ATCC 25175) suspension was obtained by pipetting one milliliter of the bacterial culture into the sterile test tube containing 1 ml of broth. Then normal saline was added gradually to it so as to compare the turbidity to that of 0.5 Mac Farland Standard that corresponded to approximately 1.5 × 108 cells/ml.[5] After sterilization of the test solutions, each tube was inoculated with 1 ml of the bacterial suspension and incubated at 37°C for 1 minute, 1 hour, 2 hours and 4 hours [Figure 1]. After the incubation period, 0.1 ml of each tube's content was plated in duplicate on BHI agar. Plates were incubated for 48 hours at 37°C. After this period, the number of colonies [Figure 2] were counted and the value of logarithm of colony forming units per milliliter was calculated (log CFU/ml).[3]

Bottom Line: Pure chicory had shown significantly less bacterial count compared to all other groups.Groups IV and V had shown significant reduction in bacterial counts over the period of 4 hrs.Chicory exerted antibacterial effect against S. mutans while coffee reduced significantly the adherence of S. mutans to the glass surface.

View Article: PubMed Central - PubMed

Affiliation: Department of Public Health Dentistry, Babu Banarasi Das College of Dental Sciences, Lucknow, Uttar Pradesh, India.

ABSTRACT

Context: Several studies have demonstrated the activity of natural plants on the dental biofilm and caries development. But few studies on the antimicrobial activity of coffee-based solutions were found in the literature. Further there was no study available to check the antimicrobial effect of coffee solutions with different percentages of chicory in it.

Aims: To evaluate the antimicrobial activity of different combinations of coffee-chicory solutions and their anti-adherence effect on Streptococcus mutans to glass surface.

Materials and methods: Test solutions were prepared. For antimicrobial activity testing, tubes containing test solution and culture medium were inoculated with a suspension of S. mutans followed by plating on Brain Heart Infusion (BHI) agar. S. mutans adherence to glass in presence of the different test solutions was also tested. The number of adhered bacteria (CFU/mL) was determined by plating method.

Statistical analysis: Statistical significance was measured using one way ANOVA followed by Tukey's post hoc test. P value < 0.05 was considered statistically significant.

Results: Pure chicory had shown significantly less bacterial count compared to all other groups. Groups IV and V had shown significant reduction in bacterial counts over the period of 4 hrs. Regarding anti-adherence effect, group I-IV had shown significantly less adherence of bacteria to glass surface.

Conclusions: Chicory exerted antibacterial effect against S. mutans while coffee reduced significantly the adherence of S. mutans to the glass surface.

No MeSH data available.


Related in: MedlinePlus