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Nuclear features in different grades of epithelial dysplasia in leukoplakia: A computer assisted microscopic study.

Gadiwan M, Madhushankari G, Mandana D, Praveen S, Selvamani M, Pradeep D - J Oral Maxillofac Pathol (2014)

Bottom Line: The purpose of this study was to observe and compare the nuclear features in different grades of epithelial dysplasia in leukoplakia and to evaluate the use of Feulgen stain for observing the nuclear features in oral epithelial dysplasia in leukoplakia.Seventy paraffin embedded tissue section (20 mild, 20 moderate, 20 severe dysplasia cases and 10 control specimens) were analyzed for nuclear morphology using Feulgen stain under trinocular research microscope.Statistically significant results were obtained with P > 0.001, when intergroup comparison was done except in case of nuclear area and diameter between mild and moderate dysplasia.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology, Krishnadevaraya College of Dental Sciences, Bangalore, Karnataka, India.

ABSTRACT

Context: Abnormalities in nuclear morphology are very frequently seen in dysplasia, such as nuclear area, diameter, shape, number of nucleoli and membrane outline. The purpose of this study was to observe and compare the nuclear features in different grades of epithelial dysplasia in leukoplakia and to evaluate the use of Feulgen stain for observing the nuclear features in oral epithelial dysplasia in leukoplakia. Seventy paraffin embedded tissue section (20 mild, 20 moderate, 20 severe dysplasia cases and 10 control specimens) were analyzed for nuclear morphology using Feulgen stain under trinocular research microscope. Statistically significant results were obtained with P > 0.001, when intergroup comparison was done except in case of nuclear area and diameter between mild and moderate dysplasia. Nuclear features reflect cell's biological potential and its morphometry was found to be a useful tool for differentiating different grades of dysplasia.

No MeSH data available.


Related in: MedlinePlus

Photomicrograph showing Feulgen stained nuclei of normal oral mucosa (Feulgen stain, ×200)
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Figure 1: Photomicrograph showing Feulgen stained nuclei of normal oral mucosa (Feulgen stain, ×200)

Mentions: Morphometry for each nucleus was done after opening the image in software and each nuclear outline was traced with the cursor without lifting the pointer once started till circle completed; the software computes the area. For diameter two straight lines were drawn across nucleus and its average was taken. Nuclear shape, membrane outline and number of nuclei were noted accordingly in a separate excel sheet for each cell [Table 1, Figures 1–6].


Nuclear features in different grades of epithelial dysplasia in leukoplakia: A computer assisted microscopic study.

Gadiwan M, Madhushankari G, Mandana D, Praveen S, Selvamani M, Pradeep D - J Oral Maxillofac Pathol (2014)

Photomicrograph showing Feulgen stained nuclei of normal oral mucosa (Feulgen stain, ×200)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4196286&req=5

Figure 1: Photomicrograph showing Feulgen stained nuclei of normal oral mucosa (Feulgen stain, ×200)
Mentions: Morphometry for each nucleus was done after opening the image in software and each nuclear outline was traced with the cursor without lifting the pointer once started till circle completed; the software computes the area. For diameter two straight lines were drawn across nucleus and its average was taken. Nuclear shape, membrane outline and number of nuclei were noted accordingly in a separate excel sheet for each cell [Table 1, Figures 1–6].

Bottom Line: The purpose of this study was to observe and compare the nuclear features in different grades of epithelial dysplasia in leukoplakia and to evaluate the use of Feulgen stain for observing the nuclear features in oral epithelial dysplasia in leukoplakia.Seventy paraffin embedded tissue section (20 mild, 20 moderate, 20 severe dysplasia cases and 10 control specimens) were analyzed for nuclear morphology using Feulgen stain under trinocular research microscope.Statistically significant results were obtained with P > 0.001, when intergroup comparison was done except in case of nuclear area and diameter between mild and moderate dysplasia.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology, Krishnadevaraya College of Dental Sciences, Bangalore, Karnataka, India.

ABSTRACT

Context: Abnormalities in nuclear morphology are very frequently seen in dysplasia, such as nuclear area, diameter, shape, number of nucleoli and membrane outline. The purpose of this study was to observe and compare the nuclear features in different grades of epithelial dysplasia in leukoplakia and to evaluate the use of Feulgen stain for observing the nuclear features in oral epithelial dysplasia in leukoplakia. Seventy paraffin embedded tissue section (20 mild, 20 moderate, 20 severe dysplasia cases and 10 control specimens) were analyzed for nuclear morphology using Feulgen stain under trinocular research microscope. Statistically significant results were obtained with P > 0.001, when intergroup comparison was done except in case of nuclear area and diameter between mild and moderate dysplasia. Nuclear features reflect cell's biological potential and its morphometry was found to be a useful tool for differentiating different grades of dysplasia.

No MeSH data available.


Related in: MedlinePlus