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Expression of Ki-67 in normal oral epithelium, leukoplakic oral epithelium and oral squamous cell carcinoma.

Birajdar SS, Radhika M, Paremala K, Sudhakara M, Soumya M, Gadivan M - J Oral Maxillofac Pathol (2014)

Bottom Line: Ki-67 positive cells in OSCC were located in the periphery of the tumor nests than the center, where frequent mitoses were observed.Ki-67 LI increased in high risk cases than low risk cases of OED.This study showed that over expression of Ki-67 antigen between well-differentiated and poorly differentiated OSCC was in accordance with histologic grade of malignancy but not in accordance with moderately differentiated OSCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology and Microbiology, Vishnu Dental College and Hospital, Vishnupur, Bhimavaram, Andhra Pradesh, India.

ABSTRACT

Aims and objective: To demonstrate the presence, location and pattern of cell proliferation in different histological grades of oral epithelial dysplasia (OED), oral squamous cell carcinoma (OSCC) and normal oral epithelium (NOE) using an antibody directed against the Ki-67 antigen and its intensity of staining evaluated respectively.

Materials and methods: A total number of 100 archival paraffin embedded blocks obtained from Department of Oral and Maxillofacial Pathology were studied. The case details were retrieved which consisted of histopathologically diagnosed cases of OSCC (n = 20), low risk OED (n = 30), high risk OED (n = 30) and normal appearing mucosa (n = 20) were taken as standard for comparison. Ki-67 immunostaining was detected. Ki-67 positive cells were counted in the five random high power fields in each case.

Results: Ki-67 labeling Index (LI) was restricted to the basal and parabasal layers of the normal oral epithelium irrespective of age, sex and site whereas it was seen in the basal, suprabasal and spinous layers in OED. Ki-67 LI is increased in high risk cases than the low risk cases of OED. Ki-67 positive cells in OSCC were located in the periphery of the tumor nests than the center, where frequent mitoses were observed.

Conclusion: The architectural alteration evaluated by Ki-67 antibody in proliferating cell distribution in the layers of epithelial dysplasias may provide useful information to evaluate the grading of OED. Ki-67 LI increased in high risk cases than low risk cases of OED. This study showed that over expression of Ki-67 antigen between well-differentiated and poorly differentiated OSCC was in accordance with histologic grade of malignancy but not in accordance with moderately differentiated OSCC.

No MeSH data available.


Related in: MedlinePlus

Comparison of Ki-67 in normal oral epithelium, oral epithelial dysplasia and Oral Squamous Cell Carcinoma cases
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Figure 3: Comparison of Ki-67 in normal oral epithelium, oral epithelial dysplasia and Oral Squamous Cell Carcinoma cases

Mentions: Ki-67 positive cells comparison between Group I [Figures 2a and b] and Group II, showed ‘P’ value of 0.0005 which is strongly significant statistically whereas between Group I and low risk Group II was not statistically significant as the sample size was more for low risk OED than NOE. This was in accordance with the study by Alfredo Maurıcio Batista de Paula et al.,[23] indicating that inflammation induces an increase in the number of epithelial cells in proliferative/cell cycle stage. Comparison between Group I and III was not statistically significant; whereas comparison between Group II and III was moderately significant statistically [Table 1 and Graph 1].


Expression of Ki-67 in normal oral epithelium, leukoplakic oral epithelium and oral squamous cell carcinoma.

Birajdar SS, Radhika M, Paremala K, Sudhakara M, Soumya M, Gadivan M - J Oral Maxillofac Pathol (2014)

Comparison of Ki-67 in normal oral epithelium, oral epithelial dysplasia and Oral Squamous Cell Carcinoma cases
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4196282&req=5

Figure 3: Comparison of Ki-67 in normal oral epithelium, oral epithelial dysplasia and Oral Squamous Cell Carcinoma cases
Mentions: Ki-67 positive cells comparison between Group I [Figures 2a and b] and Group II, showed ‘P’ value of 0.0005 which is strongly significant statistically whereas between Group I and low risk Group II was not statistically significant as the sample size was more for low risk OED than NOE. This was in accordance with the study by Alfredo Maurıcio Batista de Paula et al.,[23] indicating that inflammation induces an increase in the number of epithelial cells in proliferative/cell cycle stage. Comparison between Group I and III was not statistically significant; whereas comparison between Group II and III was moderately significant statistically [Table 1 and Graph 1].

Bottom Line: Ki-67 positive cells in OSCC were located in the periphery of the tumor nests than the center, where frequent mitoses were observed.Ki-67 LI increased in high risk cases than low risk cases of OED.This study showed that over expression of Ki-67 antigen between well-differentiated and poorly differentiated OSCC was in accordance with histologic grade of malignancy but not in accordance with moderately differentiated OSCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology and Microbiology, Vishnu Dental College and Hospital, Vishnupur, Bhimavaram, Andhra Pradesh, India.

ABSTRACT

Aims and objective: To demonstrate the presence, location and pattern of cell proliferation in different histological grades of oral epithelial dysplasia (OED), oral squamous cell carcinoma (OSCC) and normal oral epithelium (NOE) using an antibody directed against the Ki-67 antigen and its intensity of staining evaluated respectively.

Materials and methods: A total number of 100 archival paraffin embedded blocks obtained from Department of Oral and Maxillofacial Pathology were studied. The case details were retrieved which consisted of histopathologically diagnosed cases of OSCC (n = 20), low risk OED (n = 30), high risk OED (n = 30) and normal appearing mucosa (n = 20) were taken as standard for comparison. Ki-67 immunostaining was detected. Ki-67 positive cells were counted in the five random high power fields in each case.

Results: Ki-67 labeling Index (LI) was restricted to the basal and parabasal layers of the normal oral epithelium irrespective of age, sex and site whereas it was seen in the basal, suprabasal and spinous layers in OED. Ki-67 LI is increased in high risk cases than the low risk cases of OED. Ki-67 positive cells in OSCC were located in the periphery of the tumor nests than the center, where frequent mitoses were observed.

Conclusion: The architectural alteration evaluated by Ki-67 antibody in proliferating cell distribution in the layers of epithelial dysplasias may provide useful information to evaluate the grading of OED. Ki-67 LI increased in high risk cases than low risk cases of OED. This study showed that over expression of Ki-67 antigen between well-differentiated and poorly differentiated OSCC was in accordance with histologic grade of malignancy but not in accordance with moderately differentiated OSCC.

No MeSH data available.


Related in: MedlinePlus