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Immunohistochemical expression of CD34 and basic fibroblast growth factor (bFGF) in oral submucous fibrosis.

Pandiar D, Shameena P - J Oral Maxillofac Pathol (2014)

Bottom Line: Mean vascular density was found to decrease significantly as the diseases advanced.Furthermore, vascularity increased significantly in cases of OSMF turning towards malignancy.There is reduced vascularity as the disease advances and this denies the systemic absorption of carcinogens, which affects the already compromised epithelium.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology and Microbiology, Government Dental College, Kozhikode, Kerala, India.

ABSTRACT

Background: Oral submucous fibrosis (OSMF) is an insidious chronic fibrotic condition that involves the oral mucosa and occasionally the pharynx and esophagus. Vascularity in OSMF has always been a matter of debate. The prevailing concept is that epithelial atrophy occurs due to lack of perfusion but the recent data challenges this concept. Therefore, the present study was conducted to evaluate the immunoreactivity of CD34 and basic fibroblast growth factor (bFGF) in different histological grades of OSMF. This might further shed light to the role of microvasculature in OSMF, so that the epithelial atrophy and resultant malignant transformation seen in the advanced stages might be elucidated.

Materials and methods: A total of 30 cases of OSMF were included in the study and mean vascular density (MVD) was calculated using CD34 and bFGF. Five cases of OSMF with dysplasia and 2 cases of OSMF turning malignant were added during the course of the study.

Results: Mean vascular density was found to decrease significantly as the diseases advanced. Furthermore, vascularity increased significantly in cases of OSMF turning towards malignancy.

Conclusion: Our study supports the concept of epithelial atrophy aftermath of lack of perfusion. There is reduced vascularity as the disease advances and this denies the systemic absorption of carcinogens, which affects the already compromised epithelium. Consequently, liberation of angiogenic factors occurs because of malignant transformation, which explains the neoangiogenesis and increased vascularity in OSMF turning towards malignancy. Further studies are required to identify the mechanism leading to carcinogenesis in the atrophied epithelium aftermath of fibrosis and decreased vascularity.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical staining for CD34 and bFGF. (a) and (b) Early OSMF demonstrating CD34 and bFGF expression, respectively. (c) and (d) Moderately advanced OSMF demonstrating CD34 and bFGF expression, respectively. (e) and (f) Advanced OSMF demonstrating CD34 and bFGF expression, respectively (IHC stain, ×100)
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Figure 2: Immunohistochemical staining for CD34 and bFGF. (a) and (b) Early OSMF demonstrating CD34 and bFGF expression, respectively. (c) and (d) Moderately advanced OSMF demonstrating CD34 and bFGF expression, respectively. (e) and (f) Advanced OSMF demonstrating CD34 and bFGF expression, respectively (IHC stain, ×100)

Mentions: For evaluation of CD34 and bFGF expression in OSMF, the slides were examined under a compound microscope at ×400 magnification by two observers simultaneously using a double-headed microscope. The three most vascularised areas were selected under high power and the number of blood vessels for CD34 and bFGF expression in the OSMF tissue was evaluated independently by two observers [Figures 2 and 3]. Brown-stained endothelial cells (cytoplasmic expression), individually or in clusters that were clearly separate from adjacent microvessels, tumor cells or other connective tissue elements were counted as a single countable microvessel. Vessels with muscular walls were not counted. Vessel lumen and red cells, though often present, were not used to define a microvessel.[8] To evaluate the immunohistochemical expression of CD 34 and bFGF, mean of the three fields was taken as the final score.


Immunohistochemical expression of CD34 and basic fibroblast growth factor (bFGF) in oral submucous fibrosis.

Pandiar D, Shameena P - J Oral Maxillofac Pathol (2014)

Immunohistochemical staining for CD34 and bFGF. (a) and (b) Early OSMF demonstrating CD34 and bFGF expression, respectively. (c) and (d) Moderately advanced OSMF demonstrating CD34 and bFGF expression, respectively. (e) and (f) Advanced OSMF demonstrating CD34 and bFGF expression, respectively (IHC stain, ×100)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4196280&req=5

Figure 2: Immunohistochemical staining for CD34 and bFGF. (a) and (b) Early OSMF demonstrating CD34 and bFGF expression, respectively. (c) and (d) Moderately advanced OSMF demonstrating CD34 and bFGF expression, respectively. (e) and (f) Advanced OSMF demonstrating CD34 and bFGF expression, respectively (IHC stain, ×100)
Mentions: For evaluation of CD34 and bFGF expression in OSMF, the slides were examined under a compound microscope at ×400 magnification by two observers simultaneously using a double-headed microscope. The three most vascularised areas were selected under high power and the number of blood vessels for CD34 and bFGF expression in the OSMF tissue was evaluated independently by two observers [Figures 2 and 3]. Brown-stained endothelial cells (cytoplasmic expression), individually or in clusters that were clearly separate from adjacent microvessels, tumor cells or other connective tissue elements were counted as a single countable microvessel. Vessels with muscular walls were not counted. Vessel lumen and red cells, though often present, were not used to define a microvessel.[8] To evaluate the immunohistochemical expression of CD 34 and bFGF, mean of the three fields was taken as the final score.

Bottom Line: Mean vascular density was found to decrease significantly as the diseases advanced.Furthermore, vascularity increased significantly in cases of OSMF turning towards malignancy.There is reduced vascularity as the disease advances and this denies the systemic absorption of carcinogens, which affects the already compromised epithelium.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology and Microbiology, Government Dental College, Kozhikode, Kerala, India.

ABSTRACT

Background: Oral submucous fibrosis (OSMF) is an insidious chronic fibrotic condition that involves the oral mucosa and occasionally the pharynx and esophagus. Vascularity in OSMF has always been a matter of debate. The prevailing concept is that epithelial atrophy occurs due to lack of perfusion but the recent data challenges this concept. Therefore, the present study was conducted to evaluate the immunoreactivity of CD34 and basic fibroblast growth factor (bFGF) in different histological grades of OSMF. This might further shed light to the role of microvasculature in OSMF, so that the epithelial atrophy and resultant malignant transformation seen in the advanced stages might be elucidated.

Materials and methods: A total of 30 cases of OSMF were included in the study and mean vascular density (MVD) was calculated using CD34 and bFGF. Five cases of OSMF with dysplasia and 2 cases of OSMF turning malignant were added during the course of the study.

Results: Mean vascular density was found to decrease significantly as the diseases advanced. Furthermore, vascularity increased significantly in cases of OSMF turning towards malignancy.

Conclusion: Our study supports the concept of epithelial atrophy aftermath of lack of perfusion. There is reduced vascularity as the disease advances and this denies the systemic absorption of carcinogens, which affects the already compromised epithelium. Consequently, liberation of angiogenic factors occurs because of malignant transformation, which explains the neoangiogenesis and increased vascularity in OSMF turning towards malignancy. Further studies are required to identify the mechanism leading to carcinogenesis in the atrophied epithelium aftermath of fibrosis and decreased vascularity.

No MeSH data available.


Related in: MedlinePlus