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Temporal and spatial expression patterns of bone morphogenetic protein 3 in developing zebrafish.

Ito-Amano M, Nakamura Y, Morisaki M, He X, Hayashi M, Watanapokasin R, Kato H - Open Rheumatol J (2014)

Bottom Line: The expression of zbmp3 was highly detectable by real-time PCR from 1 day post-fertilization (1 dpf) to 2 weeks post-fertilization (2 wpf) and peaked at 1 wpf.It was also expressed in the pharyngeal arches, including the opercle, branchiostegal ray, and pectoral fins, at 2 dpf.Our results suggest that zbmp3 may play an important role in the skeletal biology of developing zebrafish.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto 390-8621, Japan.

ABSTRACT
Bone morphogenetic proteins (BMPs) are important elements in bone biology. We herein report the expression profiles of zebrafish bmp3 (zbmp3) as demonstrated by real-time PCR and in situ hybridization. The expression of zbmp3 was highly detectable by real-time PCR from 1 day post-fertilization (1 dpf) to 2 weeks post-fertilization (2 wpf) and peaked at 1 wpf. For in situ hybridization experiments, zbmp3 was expressed in the otic vesicle at 1 dpf, 2 dpf, 3 dpf, and 5 dpf. It was also expressed in the pharyngeal arches, including the opercle, branchiostegal ray, and pectoral fins, at 2 dpf. Our results suggest that zbmp3 may play an important role in the skeletal biology of developing zebrafish.

No MeSH data available.


Related in: MedlinePlus

Expression study of zbmp3 by real-time PCR using samples from 1 cell, 3 hpf, 8-9 hpf, 1 dpf, 2 dpf, 3 dpf, 1 wpf, and 2 wpf time points.
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Figure 1: Expression study of zbmp3 by real-time PCR using samples from 1 cell, 3 hpf, 8-9 hpf, 1 dpf, 2 dpf, 3 dpf, 1 wpf, and 2 wpf time points.

Mentions: As shown in Fig. (1), the real-time PCR expression level of zbmp3 was very low at 1 cell, 3 hpf, and 8-9 hpf time points. However, zbmp3 expression was dramatically increased from 1 dpf to 2 wpf and peaked at 1wpf.


Temporal and spatial expression patterns of bone morphogenetic protein 3 in developing zebrafish.

Ito-Amano M, Nakamura Y, Morisaki M, He X, Hayashi M, Watanapokasin R, Kato H - Open Rheumatol J (2014)

Expression study of zbmp3 by real-time PCR using samples from 1 cell, 3 hpf, 8-9 hpf, 1 dpf, 2 dpf, 3 dpf, 1 wpf, and 2 wpf time points.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4196250&req=5

Figure 1: Expression study of zbmp3 by real-time PCR using samples from 1 cell, 3 hpf, 8-9 hpf, 1 dpf, 2 dpf, 3 dpf, 1 wpf, and 2 wpf time points.
Mentions: As shown in Fig. (1), the real-time PCR expression level of zbmp3 was very low at 1 cell, 3 hpf, and 8-9 hpf time points. However, zbmp3 expression was dramatically increased from 1 dpf to 2 wpf and peaked at 1wpf.

Bottom Line: The expression of zbmp3 was highly detectable by real-time PCR from 1 day post-fertilization (1 dpf) to 2 weeks post-fertilization (2 wpf) and peaked at 1 wpf.It was also expressed in the pharyngeal arches, including the opercle, branchiostegal ray, and pectoral fins, at 2 dpf.Our results suggest that zbmp3 may play an important role in the skeletal biology of developing zebrafish.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto 390-8621, Japan.

ABSTRACT
Bone morphogenetic proteins (BMPs) are important elements in bone biology. We herein report the expression profiles of zebrafish bmp3 (zbmp3) as demonstrated by real-time PCR and in situ hybridization. The expression of zbmp3 was highly detectable by real-time PCR from 1 day post-fertilization (1 dpf) to 2 weeks post-fertilization (2 wpf) and peaked at 1 wpf. For in situ hybridization experiments, zbmp3 was expressed in the otic vesicle at 1 dpf, 2 dpf, 3 dpf, and 5 dpf. It was also expressed in the pharyngeal arches, including the opercle, branchiostegal ray, and pectoral fins, at 2 dpf. Our results suggest that zbmp3 may play an important role in the skeletal biology of developing zebrafish.

No MeSH data available.


Related in: MedlinePlus