Mycobacterial membrane vesicles administered systemically in mice induce a protective immune response to surface compartments of Mycobacterium tuberculosis.
Bottom Line: Clin.In this work, we analyzed the vaccine potential of MV in a mouse model and compared the effects of immunization with MV to those of standard BCG vaccination.The fact that MV do not need adjuvants and elicit protection comparable to that elicited by the BCG vaccine encourages vaccine approaches that combine protein antigens and lipids.
Affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx NY, USA firstname.lastname@example.org.Show MeSH
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Mentions: We tested whether immunization with mycobacterial MV was protective in mice against an M. tuberculosis challenge. Immunized mice were infected with approximately with 100 CFU via aerosol, 3 weeks after the last immunization. Eight weeks after infection, lung and spleen bacterial burdens were determined. Mice immunized with PBS or two million bacilli of BCG Pasteur were used as negative and positive controls, respectively (Fig. 4; also, see Fig. S3 in the supplemental material). The experiment was done three times. Two experiments showed a protective effect for H37Rv MV, comparable to that obtained with the control BCG, with a significant reduction of lung CFU relative to levels in PBS-treated mice (Fig. 4A). Of note, immunization with BCG MV was not protective in all experiments. In one experiment, we found almost no reduction in lung CFU in H37Rv MV-immunized mice, comparable to results with unvaccinated mice (PBS) (Fig. 4A). The same trend was observed when CFU were counted in spleens (see Fig. S3). Importantly, in experiments where H37Rv MV resulted in a reduced number of CFU in the spleen, similar to BCG, some mice had numbers of spleen CFU below the limit of detection (see Fig. S3). This result indicates the potential capacity of H37Rv MV to control bacterial replication and dissemination, similar to that of BCG.
Affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx NY, USA email@example.com.