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Nuclear herpesvirus capsid motility is not dependent on F-actin.

Bosse JB, Virding S, Thiberge SY, Scherer J, Wodrich H, Ruzsics Z, Koszinowski UH, Enquist LW - MBio (2014)

Bottom Line: However, the conclusions from these studies were indirect.Moreover, in these cells, three F-actin-inhibiting drugs failed to effect capsid motility.We revisited this phenomenon and found no evidence that nuclear capsid motility depended on F-actin.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA Princeton Neuroscience Institute, Princeton University, Princeton, New Jersey, USA.

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Representative members of all Herpesviridae subfamilies show intranuclear capsid motility. MEF cells constitutively expressing Lifeact-EGFP were infected with HSV-1 mRFP-VP26, PRV mRFP-VP26, MCMV S-mCherry-SCP, or MHV-68 mCherry-ORF65 at a multiplicity of infection (MOI) of at least 5. Live cell spinning disk microscopy with at least 3.7 frames per second was performed in one confocal section after the first capsids appeared in the nucleus at 6 hpi, 4 hpi, 20 hpi, or 8 hpi, respectively. The right column depicts a temporal maximum projection of at least 20 frames representing 5.4 s. The degree of signal smearing indicates the degree of capsid motility. Scale bar indicates 5 µm.
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fig1: Representative members of all Herpesviridae subfamilies show intranuclear capsid motility. MEF cells constitutively expressing Lifeact-EGFP were infected with HSV-1 mRFP-VP26, PRV mRFP-VP26, MCMV S-mCherry-SCP, or MHV-68 mCherry-ORF65 at a multiplicity of infection (MOI) of at least 5. Live cell spinning disk microscopy with at least 3.7 frames per second was performed in one confocal section after the first capsids appeared in the nucleus at 6 hpi, 4 hpi, 20 hpi, or 8 hpi, respectively. The right column depicts a temporal maximum projection of at least 20 frames representing 5.4 s. The degree of signal smearing indicates the degree of capsid motility. Scale bar indicates 5 µm.

Mentions: Early in infection (6 h postinfection [hpi] for HSV-1, 4 hpi for PRV, 20 hpi for MCMV, and 8 hpi for MHV-68), all four herpesviruses showed nuclear capsid motility (Fig. 1; see also Video S1 in the supplemental material), with nuclei filled with actively moving capsids, while the degree of motility declined at later time points for all four viruses. Capsids from HSV-1 and PRV showed the most motility, and capsids from MCMV and MHV-68 showed less motion (see Video S1).


Nuclear herpesvirus capsid motility is not dependent on F-actin.

Bosse JB, Virding S, Thiberge SY, Scherer J, Wodrich H, Ruzsics Z, Koszinowski UH, Enquist LW - MBio (2014)

Representative members of all Herpesviridae subfamilies show intranuclear capsid motility. MEF cells constitutively expressing Lifeact-EGFP were infected with HSV-1 mRFP-VP26, PRV mRFP-VP26, MCMV S-mCherry-SCP, or MHV-68 mCherry-ORF65 at a multiplicity of infection (MOI) of at least 5. Live cell spinning disk microscopy with at least 3.7 frames per second was performed in one confocal section after the first capsids appeared in the nucleus at 6 hpi, 4 hpi, 20 hpi, or 8 hpi, respectively. The right column depicts a temporal maximum projection of at least 20 frames representing 5.4 s. The degree of signal smearing indicates the degree of capsid motility. Scale bar indicates 5 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4196236&req=5

fig1: Representative members of all Herpesviridae subfamilies show intranuclear capsid motility. MEF cells constitutively expressing Lifeact-EGFP were infected with HSV-1 mRFP-VP26, PRV mRFP-VP26, MCMV S-mCherry-SCP, or MHV-68 mCherry-ORF65 at a multiplicity of infection (MOI) of at least 5. Live cell spinning disk microscopy with at least 3.7 frames per second was performed in one confocal section after the first capsids appeared in the nucleus at 6 hpi, 4 hpi, 20 hpi, or 8 hpi, respectively. The right column depicts a temporal maximum projection of at least 20 frames representing 5.4 s. The degree of signal smearing indicates the degree of capsid motility. Scale bar indicates 5 µm.
Mentions: Early in infection (6 h postinfection [hpi] for HSV-1, 4 hpi for PRV, 20 hpi for MCMV, and 8 hpi for MHV-68), all four herpesviruses showed nuclear capsid motility (Fig. 1; see also Video S1 in the supplemental material), with nuclei filled with actively moving capsids, while the degree of motility declined at later time points for all four viruses. Capsids from HSV-1 and PRV showed the most motility, and capsids from MCMV and MHV-68 showed less motion (see Video S1).

Bottom Line: However, the conclusions from these studies were indirect.Moreover, in these cells, three F-actin-inhibiting drugs failed to effect capsid motility.We revisited this phenomenon and found no evidence that nuclear capsid motility depended on F-actin.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA Princeton Neuroscience Institute, Princeton University, Princeton, New Jersey, USA.

Show MeSH
Related in: MedlinePlus