Septins arrange F-actin-containing fibers on the Chlamydia trachomatis inclusion and are required for normal release of the inclusion by extrusion.
Bottom Line: RNAi against SEPT9 or against the combination of SEPT2/7/9 substantially reduced the number of extrusions from a culture of infected HeLa cells.Septins have previously been found to be involved in the interaction of the cell with bacteria in the cytosol.Our observation that they also organize a reaction to bacteria living in vacuoles suggests that they have a function in the recognition of foreign compartments by a parasitized human cell.
Affiliation: Institute for Medical Microbiology and Hygiene, University Medical Center Freiburg, Freiburg, Germany.Show MeSH
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Mentions: We did observe, however, that the knockdown of SEPT2 also disrupted the fibrillar structure of SEPT9 on the inclusion (Fig. 4). This further supports the view that the three (or four) septins indeed form these structures together.
Affiliation: Institute for Medical Microbiology and Hygiene, University Medical Center Freiburg, Freiburg, Germany.