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Septins arrange F-actin-containing fibers on the Chlamydia trachomatis inclusion and are required for normal release of the inclusion by extrusion.

Volceanov L, Herbst K, Biniossek M, Schilling O, Haller D, Nölke T, Subbarayal P, Rudel T, Zieger B, Häcker G - MBio (2014)

Bottom Line: RNAi against SEPT9 or against the combination of SEPT2/7/9 substantially reduced the number of extrusions from a culture of infected HeLa cells.Septins have previously been found to be involved in the interaction of the cell with bacteria in the cytosol.Our observation that they also organize a reaction to bacteria living in vacuoles suggests that they have a function in the recognition of foreign compartments by a parasitized human cell.

View Article: PubMed Central - PubMed

Affiliation: Institute for Medical Microbiology and Hygiene, University Medical Center Freiburg, Freiburg, Germany.

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Knockdown of SEPT2 disrupts the fibrillar structure of SEPT9 on the inclusion. HeLa cells stably carrying an inducible shSEPT2 construct were induced with 100 ng/ml AHT to silence the expression of human SEPT2 for 48 h. At 24 h postinduction, cells were infected with C. trachomatis; they were fixed at 30 h postinfection and labeled with an antibody against SEPT9. Inclusions are indicated by arrows. Cell nuclei are marked with asterisks. Scale bar, 10 µm. Images are representative of 3 independent experiments.
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fig4: Knockdown of SEPT2 disrupts the fibrillar structure of SEPT9 on the inclusion. HeLa cells stably carrying an inducible shSEPT2 construct were induced with 100 ng/ml AHT to silence the expression of human SEPT2 for 48 h. At 24 h postinduction, cells were infected with C. trachomatis; they were fixed at 30 h postinfection and labeled with an antibody against SEPT9. Inclusions are indicated by arrows. Cell nuclei are marked with asterisks. Scale bar, 10 µm. Images are representative of 3 independent experiments.

Mentions: We did observe, however, that the knockdown of SEPT2 also disrupted the fibrillar structure of SEPT9 on the inclusion (Fig. 4). This further supports the view that the three (or four) septins indeed form these structures together.


Septins arrange F-actin-containing fibers on the Chlamydia trachomatis inclusion and are required for normal release of the inclusion by extrusion.

Volceanov L, Herbst K, Biniossek M, Schilling O, Haller D, Nölke T, Subbarayal P, Rudel T, Zieger B, Häcker G - MBio (2014)

Knockdown of SEPT2 disrupts the fibrillar structure of SEPT9 on the inclusion. HeLa cells stably carrying an inducible shSEPT2 construct were induced with 100 ng/ml AHT to silence the expression of human SEPT2 for 48 h. At 24 h postinduction, cells were infected with C. trachomatis; they were fixed at 30 h postinfection and labeled with an antibody against SEPT9. Inclusions are indicated by arrows. Cell nuclei are marked with asterisks. Scale bar, 10 µm. Images are representative of 3 independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4196233&req=5

fig4: Knockdown of SEPT2 disrupts the fibrillar structure of SEPT9 on the inclusion. HeLa cells stably carrying an inducible shSEPT2 construct were induced with 100 ng/ml AHT to silence the expression of human SEPT2 for 48 h. At 24 h postinduction, cells were infected with C. trachomatis; they were fixed at 30 h postinfection and labeled with an antibody against SEPT9. Inclusions are indicated by arrows. Cell nuclei are marked with asterisks. Scale bar, 10 µm. Images are representative of 3 independent experiments.
Mentions: We did observe, however, that the knockdown of SEPT2 also disrupted the fibrillar structure of SEPT9 on the inclusion (Fig. 4). This further supports the view that the three (or four) septins indeed form these structures together.

Bottom Line: RNAi against SEPT9 or against the combination of SEPT2/7/9 substantially reduced the number of extrusions from a culture of infected HeLa cells.Septins have previously been found to be involved in the interaction of the cell with bacteria in the cytosol.Our observation that they also organize a reaction to bacteria living in vacuoles suggests that they have a function in the recognition of foreign compartments by a parasitized human cell.

View Article: PubMed Central - PubMed

Affiliation: Institute for Medical Microbiology and Hygiene, University Medical Center Freiburg, Freiburg, Germany.

Show MeSH
Related in: MedlinePlus