Limits...
Septins arrange F-actin-containing fibers on the Chlamydia trachomatis inclusion and are required for normal release of the inclusion by extrusion.

Volceanov L, Herbst K, Biniossek M, Schilling O, Haller D, Nölke T, Subbarayal P, Rudel T, Zieger B, Häcker G - MBio (2014)

Bottom Line: RNAi against SEPT9 or against the combination of SEPT2/7/9 substantially reduced the number of extrusions from a culture of infected HeLa cells.Septins have previously been found to be involved in the interaction of the cell with bacteria in the cytosol.Our observation that they also organize a reaction to bacteria living in vacuoles suggests that they have a function in the recognition of foreign compartments by a parasitized human cell.

View Article: PubMed Central - PubMed

Affiliation: Institute for Medical Microbiology and Hygiene, University Medical Center Freiburg, Freiburg, Germany.

Show MeSH

Related in: MedlinePlus

SEPT2 forms filamentous structures around the chlamydial inclusion. HeLa cells were infected with C. trachomatis for 16, 20, 24, or 39 h. Immunostaining for SEPT2 (red) and chlamydial Hsp60 (cHsp60) (green) was performed. Arrows point to chlamydial inclusions. Scale bar, 10 µm. Images are representative of 2 individual experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4196233&req=5

fig1: SEPT2 forms filamentous structures around the chlamydial inclusion. HeLa cells were infected with C. trachomatis for 16, 20, 24, or 39 h. Immunostaining for SEPT2 (red) and chlamydial Hsp60 (cHsp60) (green) was performed. Arrows point to chlamydial inclusions. Scale bar, 10 µm. Images are representative of 2 individual experiments.

Mentions: This finding prompted us to investigate the structure of septins during chlamydial infection. Immunostaining for SEPT2 showed that SEPT2-containing fibers encased the chlamydial inclusion, starting from about 16 h postinfection (p.i.) (Fig. 1). In most cells, fibers that ran across the inclusion were observed, often visible as a ring around the inclusion in epifluorescence microscopy. Confocal imaging and orthogonal views of the reconstructed image show fibers containing SEPT2 (see Fig. S2A and B in the supplemental material) and SEPT9 (see Fig. S2C) at the bottom of the inclusion, around it, and at its top. The structures are suggestive of larger-order structures since they are well known to be formed by septins.


Septins arrange F-actin-containing fibers on the Chlamydia trachomatis inclusion and are required for normal release of the inclusion by extrusion.

Volceanov L, Herbst K, Biniossek M, Schilling O, Haller D, Nölke T, Subbarayal P, Rudel T, Zieger B, Häcker G - MBio (2014)

SEPT2 forms filamentous structures around the chlamydial inclusion. HeLa cells were infected with C. trachomatis for 16, 20, 24, or 39 h. Immunostaining for SEPT2 (red) and chlamydial Hsp60 (cHsp60) (green) was performed. Arrows point to chlamydial inclusions. Scale bar, 10 µm. Images are representative of 2 individual experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4196233&req=5

fig1: SEPT2 forms filamentous structures around the chlamydial inclusion. HeLa cells were infected with C. trachomatis for 16, 20, 24, or 39 h. Immunostaining for SEPT2 (red) and chlamydial Hsp60 (cHsp60) (green) was performed. Arrows point to chlamydial inclusions. Scale bar, 10 µm. Images are representative of 2 individual experiments.
Mentions: This finding prompted us to investigate the structure of septins during chlamydial infection. Immunostaining for SEPT2 showed that SEPT2-containing fibers encased the chlamydial inclusion, starting from about 16 h postinfection (p.i.) (Fig. 1). In most cells, fibers that ran across the inclusion were observed, often visible as a ring around the inclusion in epifluorescence microscopy. Confocal imaging and orthogonal views of the reconstructed image show fibers containing SEPT2 (see Fig. S2A and B in the supplemental material) and SEPT9 (see Fig. S2C) at the bottom of the inclusion, around it, and at its top. The structures are suggestive of larger-order structures since they are well known to be formed by septins.

Bottom Line: RNAi against SEPT9 or against the combination of SEPT2/7/9 substantially reduced the number of extrusions from a culture of infected HeLa cells.Septins have previously been found to be involved in the interaction of the cell with bacteria in the cytosol.Our observation that they also organize a reaction to bacteria living in vacuoles suggests that they have a function in the recognition of foreign compartments by a parasitized human cell.

View Article: PubMed Central - PubMed

Affiliation: Institute for Medical Microbiology and Hygiene, University Medical Center Freiburg, Freiburg, Germany.

Show MeSH
Related in: MedlinePlus