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Differential genome evolution between companion symbionts in an insect-bacterial symbiosis.

Bennett GM, McCutcheon JP, MacDonald BR, Romanovicz D, Moran NA - MBio (2014)

Bottom Line: Phylogenomic results suggest that "Ca.Taken together, our results suggest that the two "Ca.We found that the older symbiont has a highly reduced genome with low rates of mutation and gene loss.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Biology, University of Texas, Austin, Texas, USA gordon.bennett@utexas.edu.

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Related in: MedlinePlus

Comparison of “Ca. Baumannia cicadellinicola” genomes from two different sharpshooter leafhopper host species, Graphocephala atropunctata (blue-green sharpshooter [BGSS]) and the previously sequenced Homalodisca vitripennis (glassy-winged sharpshooter [GWSS]) (11). Genes are color-coded as core shared genes or unique to BGSS or GWSS “Ca. Baumannia” as indicated in the key. The genomes are completely syntenic except for large contiguous gene deletions. The graph on the inner track shows genomewide GC skew, and the outer track shows predicted genes for each genome. Genes are color-coded according to whether they are unique to a genome (e.g., blue for unique BGSS genes) or whether they are shared between genomes (grey). Black tick marks around the outer track show inferred origins of replication.
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fig1: Comparison of “Ca. Baumannia cicadellinicola” genomes from two different sharpshooter leafhopper host species, Graphocephala atropunctata (blue-green sharpshooter [BGSS]) and the previously sequenced Homalodisca vitripennis (glassy-winged sharpshooter [GWSS]) (11). Genes are color-coded as core shared genes or unique to BGSS or GWSS “Ca. Baumannia” as indicated in the key. The genomes are completely syntenic except for large contiguous gene deletions. The graph on the inner track shows genomewide GC skew, and the outer track shows predicted genes for each genome. Genes are color-coded according to whether they are unique to a genome (e.g., blue for unique BGSS genes) or whether they are shared between genomes (grey). Black tick marks around the outer track show inferred origins of replication.

Mentions: The genome sizes for S-BGSS and B-BGSS are 244,618 and 759,425 bp, respectively (Fig. 1). Both symbionts have low GC content, which is typical in obligate symbioses: the GC content is 17% for S-BGSS and 39% for B-BGSS. B-BGSS contains 694 predicted protein-coding sequences (CDS), of which 20 encode hypothetical proteins. It contains two ribosomal cassettes, 39 tRNAs, and 10 CDS that are truncated or pseudogenized with uncertain function (see Table S2 in the supplemental material). S-BGSS has 225 CDS, with 10 encoding hypothetical proteins, a single ribosomal cassette, 31 tRNAs, and no identifiable pseudogenes (Table S3). Figure 2 shows clusters of orthologous groups (COG) assignments for genes in both symbiont genomes from BGSS and GWSS. For B-BGSS, 69 genes could not be assigned.


Differential genome evolution between companion symbionts in an insect-bacterial symbiosis.

Bennett GM, McCutcheon JP, MacDonald BR, Romanovicz D, Moran NA - MBio (2014)

Comparison of “Ca. Baumannia cicadellinicola” genomes from two different sharpshooter leafhopper host species, Graphocephala atropunctata (blue-green sharpshooter [BGSS]) and the previously sequenced Homalodisca vitripennis (glassy-winged sharpshooter [GWSS]) (11). Genes are color-coded as core shared genes or unique to BGSS or GWSS “Ca. Baumannia” as indicated in the key. The genomes are completely syntenic except for large contiguous gene deletions. The graph on the inner track shows genomewide GC skew, and the outer track shows predicted genes for each genome. Genes are color-coded according to whether they are unique to a genome (e.g., blue for unique BGSS genes) or whether they are shared between genomes (grey). Black tick marks around the outer track show inferred origins of replication.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4196230&req=5

fig1: Comparison of “Ca. Baumannia cicadellinicola” genomes from two different sharpshooter leafhopper host species, Graphocephala atropunctata (blue-green sharpshooter [BGSS]) and the previously sequenced Homalodisca vitripennis (glassy-winged sharpshooter [GWSS]) (11). Genes are color-coded as core shared genes or unique to BGSS or GWSS “Ca. Baumannia” as indicated in the key. The genomes are completely syntenic except for large contiguous gene deletions. The graph on the inner track shows genomewide GC skew, and the outer track shows predicted genes for each genome. Genes are color-coded according to whether they are unique to a genome (e.g., blue for unique BGSS genes) or whether they are shared between genomes (grey). Black tick marks around the outer track show inferred origins of replication.
Mentions: The genome sizes for S-BGSS and B-BGSS are 244,618 and 759,425 bp, respectively (Fig. 1). Both symbionts have low GC content, which is typical in obligate symbioses: the GC content is 17% for S-BGSS and 39% for B-BGSS. B-BGSS contains 694 predicted protein-coding sequences (CDS), of which 20 encode hypothetical proteins. It contains two ribosomal cassettes, 39 tRNAs, and 10 CDS that are truncated or pseudogenized with uncertain function (see Table S2 in the supplemental material). S-BGSS has 225 CDS, with 10 encoding hypothetical proteins, a single ribosomal cassette, 31 tRNAs, and no identifiable pseudogenes (Table S3). Figure 2 shows clusters of orthologous groups (COG) assignments for genes in both symbiont genomes from BGSS and GWSS. For B-BGSS, 69 genes could not be assigned.

Bottom Line: Phylogenomic results suggest that "Ca.Taken together, our results suggest that the two "Ca.We found that the older symbiont has a highly reduced genome with low rates of mutation and gene loss.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Biology, University of Texas, Austin, Texas, USA gordon.bennett@utexas.edu.

Show MeSH
Related in: MedlinePlus