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Absence of patient-to-patient intrahospital transmission of Staphylococcus aureus as determined by whole-genome sequencing.

Long SW, Beres SB, Olsen RJ, Musser JM - MBio (2014)

Bottom Line: To identify patient-to-patient intrahospital transmission using high-resolution genetic analysis, we sequenced the genomes of a consecutive set of 398 S. aureus isolates from sterile-site infections.The S. aureus strains were collected from four hospitals in the Houston Methodist Hospital System over a 6-month period.In addition, our data demonstrate that highly related pools of S. aureus strains exist in the community which may complicate outbreak investigations.

View Article: PubMed Central - PubMed

Affiliation: jmmusser@houstonmethodist.org.

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Related in: MedlinePlus

Radial phylogenetic tree of the 3 CC75 HMHS-SA isolates. A clear relationship is evident between HMHS-SA-57 and HMHS-SA-328, which were isolated from the same patient several months apart, compared to HMHS-SA-153. Isolate HMHS-SA-153 was typed as ST1223 by SRST2. HMHS-SA-57 and HMHS-SA-328 share a novel MLST type, which is a single locus variant of ST2793.
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fig5: Radial phylogenetic tree of the 3 CC75 HMHS-SA isolates. A clear relationship is evident between HMHS-SA-57 and HMHS-SA-328, which were isolated from the same patient several months apart, compared to HMHS-SA-153. Isolate HMHS-SA-153 was typed as ST1223 by SRST2. HMHS-SA-57 and HMHS-SA-328 share a novel MLST type, which is a single locus variant of ST2793.

Mentions: S. aureus CC75 is a highly genetically divergent lineage that has been reported only in Australia, Cambodia, and South America (39–42). Unexpectedly, our Houston data set included three isolates that clustered with CC75, thus representing the first report of these organisms in North America. Interestingly, our three CC75 isolates are phylogenetically divergent (17,002 to 20,915 SNPs) from the only available CC75 reference genome, MSHR1132 (Fig. 5) (39). Consequently, these newly identified clones may represent the emergence of a novel clonal complex in North America.


Absence of patient-to-patient intrahospital transmission of Staphylococcus aureus as determined by whole-genome sequencing.

Long SW, Beres SB, Olsen RJ, Musser JM - MBio (2014)

Radial phylogenetic tree of the 3 CC75 HMHS-SA isolates. A clear relationship is evident between HMHS-SA-57 and HMHS-SA-328, which were isolated from the same patient several months apart, compared to HMHS-SA-153. Isolate HMHS-SA-153 was typed as ST1223 by SRST2. HMHS-SA-57 and HMHS-SA-328 share a novel MLST type, which is a single locus variant of ST2793.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4196229&req=5

fig5: Radial phylogenetic tree of the 3 CC75 HMHS-SA isolates. A clear relationship is evident between HMHS-SA-57 and HMHS-SA-328, which were isolated from the same patient several months apart, compared to HMHS-SA-153. Isolate HMHS-SA-153 was typed as ST1223 by SRST2. HMHS-SA-57 and HMHS-SA-328 share a novel MLST type, which is a single locus variant of ST2793.
Mentions: S. aureus CC75 is a highly genetically divergent lineage that has been reported only in Australia, Cambodia, and South America (39–42). Unexpectedly, our Houston data set included three isolates that clustered with CC75, thus representing the first report of these organisms in North America. Interestingly, our three CC75 isolates are phylogenetically divergent (17,002 to 20,915 SNPs) from the only available CC75 reference genome, MSHR1132 (Fig. 5) (39). Consequently, these newly identified clones may represent the emergence of a novel clonal complex in North America.

Bottom Line: To identify patient-to-patient intrahospital transmission using high-resolution genetic analysis, we sequenced the genomes of a consecutive set of 398 S. aureus isolates from sterile-site infections.The S. aureus strains were collected from four hospitals in the Houston Methodist Hospital System over a 6-month period.In addition, our data demonstrate that highly related pools of S. aureus strains exist in the community which may complicate outbreak investigations.

View Article: PubMed Central - PubMed

Affiliation: jmmusser@houstonmethodist.org.

Show MeSH
Related in: MedlinePlus