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Absence of patient-to-patient intrahospital transmission of Staphylococcus aureus as determined by whole-genome sequencing.

Long SW, Beres SB, Olsen RJ, Musser JM - MBio (2014)

Bottom Line: To identify patient-to-patient intrahospital transmission using high-resolution genetic analysis, we sequenced the genomes of a consecutive set of 398 S. aureus isolates from sterile-site infections.The S. aureus strains were collected from four hospitals in the Houston Methodist Hospital System over a 6-month period.In addition, our data demonstrate that highly related pools of S. aureus strains exist in the community which may complicate outbreak investigations.

View Article: PubMed Central - PubMed

Affiliation: jmmusser@houstonmethodist.org.

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Related in: MedlinePlus

Circular cladogram colored by MLST. All MLST STs of a given clonal complex are colored shades of the same color. MLST was determined using a combination of SRST2 software and manual sequence review. Strains with a novel MLST allele type are labeled in gray (“Novel”).
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fig2: Circular cladogram colored by MLST. All MLST STs of a given clonal complex are colored shades of the same color. MLST was determined using a combination of SRST2 software and manual sequence review. Strains with a novel MLST allele type are labeled in gray (“Novel”).

Mentions: To correlate the genome data with traditional typing methods, the MLST types of the isolates were computationally determined and mapped onto the phylogenetic tree (Fig. 2). Predictably, strains with the same MLST type clustered into the same distinct branch. This analysis confirmed that there was a considerable fine-structure genomic diversity among strains assigned to the same MLST. Moreover, it reaffirmed a conclusion by Harris et al. (25) that, compared to lower-resolution typing methods, whole-genome sequencing establishes a greater degree of genetic divergence that can enhance identification of intrahospital transmission events.


Absence of patient-to-patient intrahospital transmission of Staphylococcus aureus as determined by whole-genome sequencing.

Long SW, Beres SB, Olsen RJ, Musser JM - MBio (2014)

Circular cladogram colored by MLST. All MLST STs of a given clonal complex are colored shades of the same color. MLST was determined using a combination of SRST2 software and manual sequence review. Strains with a novel MLST allele type are labeled in gray (“Novel”).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4196229&req=5

fig2: Circular cladogram colored by MLST. All MLST STs of a given clonal complex are colored shades of the same color. MLST was determined using a combination of SRST2 software and manual sequence review. Strains with a novel MLST allele type are labeled in gray (“Novel”).
Mentions: To correlate the genome data with traditional typing methods, the MLST types of the isolates were computationally determined and mapped onto the phylogenetic tree (Fig. 2). Predictably, strains with the same MLST type clustered into the same distinct branch. This analysis confirmed that there was a considerable fine-structure genomic diversity among strains assigned to the same MLST. Moreover, it reaffirmed a conclusion by Harris et al. (25) that, compared to lower-resolution typing methods, whole-genome sequencing establishes a greater degree of genetic divergence that can enhance identification of intrahospital transmission events.

Bottom Line: To identify patient-to-patient intrahospital transmission using high-resolution genetic analysis, we sequenced the genomes of a consecutive set of 398 S. aureus isolates from sterile-site infections.The S. aureus strains were collected from four hospitals in the Houston Methodist Hospital System over a 6-month period.In addition, our data demonstrate that highly related pools of S. aureus strains exist in the community which may complicate outbreak investigations.

View Article: PubMed Central - PubMed

Affiliation: jmmusser@houstonmethodist.org.

Show MeSH
Related in: MedlinePlus