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Metagenome-wide association of microbial determinants of host phenotype in Drosophila melanogaster.

Chaston JM, Newell PD, Douglas AE - MBio (2014)

Bottom Line: The effects of monoassociation on host traits were not confined to particular taxonomic groups.This analysis showed significant effects of pyrroloquinoline quinone biosynthesis genes on development time, confirming the results of a published transposon mutagenesis screen, thereby validating the MGWA; it also identified multiple genes predicted to affect host TAG content, including extracellular glucose oxidation pathway components.The genomes of the chosen bacterial strains were previously sequenced, so we could statistically associate specific bacterial genes with bacterially mediated reduction in host fat content.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, Cornell University, Ithaca, New York, USA john_chaston@byu.edu.

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Larval development time and TAG content in monoassociated D. melanogaster flies. Traits for D. melanogaster that were monoassociated with each of 41 bacterial strains were measured. Phylogenetic trees were calculated using 16S sequences with unweighted branch lengths. Taxon abbreviations are defined in Table 1. Significant differences between treatments after Bonferroni correction (P < 0.05) are indicated by different letters next to bars. (A) Differences in bacterial effects on larval time to pupariation (development time) were observed between strains. Survival analysis using a Cox mixed model was used to identify significant differences between treatments, with experimental replicate and vial as random effects. To facilitate visualization, data are presented as the mean times to development ± standard errors of the means (SEM). (B) Differences in bacterial effects on TAG content were observed between strains. A linear mixed model was used to identify significant differences between treatments, with experimental replicate as a random effect. Data are presented as mean TAG content ± SEM (mean of experimental means). Red, Proteobacteria; blue, Firmicutes; gray, Bacteroidetes.
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fig1: Larval development time and TAG content in monoassociated D. melanogaster flies. Traits for D. melanogaster that were monoassociated with each of 41 bacterial strains were measured. Phylogenetic trees were calculated using 16S sequences with unweighted branch lengths. Taxon abbreviations are defined in Table 1. Significant differences between treatments after Bonferroni correction (P < 0.05) are indicated by different letters next to bars. (A) Differences in bacterial effects on larval time to pupariation (development time) were observed between strains. Survival analysis using a Cox mixed model was used to identify significant differences between treatments, with experimental replicate and vial as random effects. To facilitate visualization, data are presented as the mean times to development ± standard errors of the means (SEM). (B) Differences in bacterial effects on TAG content were observed between strains. A linear mixed model was used to identify significant differences between treatments, with experimental replicate as a random effect. Data are presented as mean TAG content ± SEM (mean of experimental means). Red, Proteobacteria; blue, Firmicutes; gray, Bacteroidetes.

Mentions: The abbreviations are used in Fig. 1 and in the supplemental material.


Metagenome-wide association of microbial determinants of host phenotype in Drosophila melanogaster.

Chaston JM, Newell PD, Douglas AE - MBio (2014)

Larval development time and TAG content in monoassociated D. melanogaster flies. Traits for D. melanogaster that were monoassociated with each of 41 bacterial strains were measured. Phylogenetic trees were calculated using 16S sequences with unweighted branch lengths. Taxon abbreviations are defined in Table 1. Significant differences between treatments after Bonferroni correction (P < 0.05) are indicated by different letters next to bars. (A) Differences in bacterial effects on larval time to pupariation (development time) were observed between strains. Survival analysis using a Cox mixed model was used to identify significant differences between treatments, with experimental replicate and vial as random effects. To facilitate visualization, data are presented as the mean times to development ± standard errors of the means (SEM). (B) Differences in bacterial effects on TAG content were observed between strains. A linear mixed model was used to identify significant differences between treatments, with experimental replicate as a random effect. Data are presented as mean TAG content ± SEM (mean of experimental means). Red, Proteobacteria; blue, Firmicutes; gray, Bacteroidetes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4196228&req=5

fig1: Larval development time and TAG content in monoassociated D. melanogaster flies. Traits for D. melanogaster that were monoassociated with each of 41 bacterial strains were measured. Phylogenetic trees were calculated using 16S sequences with unweighted branch lengths. Taxon abbreviations are defined in Table 1. Significant differences between treatments after Bonferroni correction (P < 0.05) are indicated by different letters next to bars. (A) Differences in bacterial effects on larval time to pupariation (development time) were observed between strains. Survival analysis using a Cox mixed model was used to identify significant differences between treatments, with experimental replicate and vial as random effects. To facilitate visualization, data are presented as the mean times to development ± standard errors of the means (SEM). (B) Differences in bacterial effects on TAG content were observed between strains. A linear mixed model was used to identify significant differences between treatments, with experimental replicate as a random effect. Data are presented as mean TAG content ± SEM (mean of experimental means). Red, Proteobacteria; blue, Firmicutes; gray, Bacteroidetes.
Mentions: The abbreviations are used in Fig. 1 and in the supplemental material.

Bottom Line: The effects of monoassociation on host traits were not confined to particular taxonomic groups.This analysis showed significant effects of pyrroloquinoline quinone biosynthesis genes on development time, confirming the results of a published transposon mutagenesis screen, thereby validating the MGWA; it also identified multiple genes predicted to affect host TAG content, including extracellular glucose oxidation pathway components.The genomes of the chosen bacterial strains were previously sequenced, so we could statistically associate specific bacterial genes with bacterially mediated reduction in host fat content.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, Cornell University, Ithaca, New York, USA john_chaston@byu.edu.

Show MeSH
Related in: MedlinePlus