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Immunomodulatory effects of Newcastle disease virus AF2240 strain on human peripheral blood mononuclear cells.

Lam HY, Yusoff K, Yeap SK, Subramani T, Abd-Aziz S, Omar AR, Alitheen NB - Int J Med Sci (2014)

Bottom Line: In this study, the immunomodulatory effects of low titers of Newcastle disease virus (NDV) AF2240 on human peripheral blood mononuclear cells (PBMC) were analyzed.Human PBMC treated with NDV titer 8 HAU was found to stimulate the highest level of cytokine production including interferon-γ, interleukin-2 and interleukin-12.The release of these proteins contributes to the antitumor effect of PBMC against MCF-7 breast cancer cells.

View Article: PubMed Central - PubMed

Affiliation: 1. Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.

ABSTRACT
Immunotherapy has raised the attention of many scientists because it hold promise to be an attractive therapeutic strategy to treat a number of disorders. In this study, the immunomodulatory effects of low titers of Newcastle disease virus (NDV) AF2240 on human peripheral blood mononuclear cells (PBMC) were analyzed. We evaluated cytokine secretion and PBMC activation by cell proliferation assay, immunophenotyping and enzyme linked immunosorbent assay. The proliferation of the human PBMC was measured to be 28.5% and 36.5% upon treatment with 8 hemaglutinin unit (HAU) and 2 HAU of NDV respectively. Interestingly, the percentage of cells with activating markers CD16 and CD56 were increased significantly. Furthermore, the intracellular perforin and granzyme levels were also increased upon virus infection. Human PBMC treated with NDV titer 8 HAU was found to stimulate the highest level of cytokine production including interferon-γ, interleukin-2 and interleukin-12. The release of these proteins contributes to the antitumor effect of PBMC against MCF-7 breast cancer cells. Based on the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay, activated human PBMC showed high cytolytic efficiency towards human breast tumor cells. In summary, NDV was able to stimulate PBMC proliferation, cytokine secretion and cytolytic activity.

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BrdU cell proliferation study of human peripheral blood mononuclear cells (PBMC) after exposed to NDV for 72 hours.The values were the means ± SE of three independent experiment. The differences between the control group and treated group were determined by one-way ANOVA. (* p < 0.05).
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Figure 1: BrdU cell proliferation study of human peripheral blood mononuclear cells (PBMC) after exposed to NDV for 72 hours.The values were the means ± SE of three independent experiment. The differences between the control group and treated group were determined by one-way ANOVA. (* p < 0.05).

Mentions: Cell proliferation was investigated to determine the immunostimulatory effects of NDV using BrdU cell proliferation assay by measuring the incorporation of thymidine analog Bromodeoxyuridine. BrdU is incorporated into the newly synthesized DNA of replicating cells during the synthesis phase of the cell cycle, indicating that the cells are actively replicating their DNAs. Exposure to NDV by human PBMC increased the cell proliferation by 28±7.329% and 36±13.033% after being treated with virus titer 8 HAU and 2 HAU respectively for 72 hours compared to untreated control cells (Figure 1). These results clearly reflected the increase of proliferation potency upon virus treatment.


Immunomodulatory effects of Newcastle disease virus AF2240 strain on human peripheral blood mononuclear cells.

Lam HY, Yusoff K, Yeap SK, Subramani T, Abd-Aziz S, Omar AR, Alitheen NB - Int J Med Sci (2014)

BrdU cell proliferation study of human peripheral blood mononuclear cells (PBMC) after exposed to NDV for 72 hours.The values were the means ± SE of three independent experiment. The differences between the control group and treated group were determined by one-way ANOVA. (* p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4196125&req=5

Figure 1: BrdU cell proliferation study of human peripheral blood mononuclear cells (PBMC) after exposed to NDV for 72 hours.The values were the means ± SE of three independent experiment. The differences between the control group and treated group were determined by one-way ANOVA. (* p < 0.05).
Mentions: Cell proliferation was investigated to determine the immunostimulatory effects of NDV using BrdU cell proliferation assay by measuring the incorporation of thymidine analog Bromodeoxyuridine. BrdU is incorporated into the newly synthesized DNA of replicating cells during the synthesis phase of the cell cycle, indicating that the cells are actively replicating their DNAs. Exposure to NDV by human PBMC increased the cell proliferation by 28±7.329% and 36±13.033% after being treated with virus titer 8 HAU and 2 HAU respectively for 72 hours compared to untreated control cells (Figure 1). These results clearly reflected the increase of proliferation potency upon virus treatment.

Bottom Line: In this study, the immunomodulatory effects of low titers of Newcastle disease virus (NDV) AF2240 on human peripheral blood mononuclear cells (PBMC) were analyzed.Human PBMC treated with NDV titer 8 HAU was found to stimulate the highest level of cytokine production including interferon-γ, interleukin-2 and interleukin-12.The release of these proteins contributes to the antitumor effect of PBMC against MCF-7 breast cancer cells.

View Article: PubMed Central - PubMed

Affiliation: 1. Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.

ABSTRACT
Immunotherapy has raised the attention of many scientists because it hold promise to be an attractive therapeutic strategy to treat a number of disorders. In this study, the immunomodulatory effects of low titers of Newcastle disease virus (NDV) AF2240 on human peripheral blood mononuclear cells (PBMC) were analyzed. We evaluated cytokine secretion and PBMC activation by cell proliferation assay, immunophenotyping and enzyme linked immunosorbent assay. The proliferation of the human PBMC was measured to be 28.5% and 36.5% upon treatment with 8 hemaglutinin unit (HAU) and 2 HAU of NDV respectively. Interestingly, the percentage of cells with activating markers CD16 and CD56 were increased significantly. Furthermore, the intracellular perforin and granzyme levels were also increased upon virus infection. Human PBMC treated with NDV titer 8 HAU was found to stimulate the highest level of cytokine production including interferon-γ, interleukin-2 and interleukin-12. The release of these proteins contributes to the antitumor effect of PBMC against MCF-7 breast cancer cells. Based on the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay, activated human PBMC showed high cytolytic efficiency towards human breast tumor cells. In summary, NDV was able to stimulate PBMC proliferation, cytokine secretion and cytolytic activity.

Show MeSH
Related in: MedlinePlus