Limits...
Detection of platelet-monocyte aggregates by the ADAM(®) image cytometer.

Jung BK, Cho CH, Moon KC, Sung Hur D, Yoon JA, Yoon SY - Int J Med Sci (2014)

Bottom Line: Moreover, the PMA measurements made by the ADAM(®) cytometer exhibited a high correlation with those made by a flow cytometric assay (R = 0.944).The ADAM(®) cytometer is a suitable alternative method to the flow cytometry-based assays.This, in turn, will provide valuable information regarding patient propensities to thrombotic diseases.

View Article: PubMed Central - PubMed

Affiliation: 1. Department of Laboratory Medicine, College of Medicine, Korea University Guro Hospital, Seoul 152-703, South Korea.

ABSTRACT

Background: Inappropriate platelet activation is known to be associated with various thrombotic disorders. Platelet-monocyte aggregates (PMAs), whose formation is mediated by platelet surface P-selectin (CD62P), can be used as a reliable marker to detect platelet activation. Previous studies have generally detected PMAs through flow cytometry-based approaches. Recently, the ADAM(®) image cytometer (Nanoentek Inc., Seoul, Korea) was developed for image-based cellular analysis. In this study, we detected PMAs with the ADAM(®) cytometer, evaluated the reproducibility of the measurements made by the ADAM(®) cytometer, and compared the abilities of the ADAM(®) cytometer and a flow cytometric assay to detect PMAs.

Methods: Whole blood samples were collected from patients. Within 5 minutes of collection, anticoagulated whole blood samples were fixed in 10% paraformaldehyde and 5% glyoxal. Nineteen clinical specimens were collected; each was analyzed three times with the ADAM(®) cytometer in order to assess the reproducibility of its measurements. To compare the ability of the ADAM(®) cytometer with that of a flow cytometer to detect PMAs, each cytometer was used for 23 clinical samples and the correlation of the measurements was determined.

Results: The PMA measurements made by the ADAM(®) cytometer showed good reproducibility (CV < 10% for all specimens). Moreover, the PMA measurements made by the ADAM(®) cytometer exhibited a high correlation with those made by a flow cytometric assay (R = 0.944).

Conclusions: The ADAM(®) cytometer is a suitable alternative method to the flow cytometry-based assays. Since the ADAM cytometer does not need specialized instrument knowledge or software proficiency (unlike flow cytometry), the ADAM(®) cytometer can be used as a rapid and reliable POCT device to measure platelet activation in peripheral blood. This, in turn, will provide valuable information regarding patient propensities to thrombotic diseases.

Show MeSH

Related in: MedlinePlus

Correlation between PMA measurements obtained with the ADAM® cytometer vs those obtained with a flow cytometer. The ADAM® measurements correlated well with the flow cytometric measurements (R = 0.944).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4196123&req=5

Figure 3: Correlation between PMA measurements obtained with the ADAM® cytometer vs those obtained with a flow cytometer. The ADAM® measurements correlated well with the flow cytometric measurements (R = 0.944).

Mentions: Twenty-three blood samples were evaluated. The measurements made by the ADAM® image cytometer correlated well with those made using the flow cytometric assay to detect PMAs (R = 0.944) (Fig. 3).


Detection of platelet-monocyte aggregates by the ADAM(®) image cytometer.

Jung BK, Cho CH, Moon KC, Sung Hur D, Yoon JA, Yoon SY - Int J Med Sci (2014)

Correlation between PMA measurements obtained with the ADAM® cytometer vs those obtained with a flow cytometer. The ADAM® measurements correlated well with the flow cytometric measurements (R = 0.944).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4196123&req=5

Figure 3: Correlation between PMA measurements obtained with the ADAM® cytometer vs those obtained with a flow cytometer. The ADAM® measurements correlated well with the flow cytometric measurements (R = 0.944).
Mentions: Twenty-three blood samples were evaluated. The measurements made by the ADAM® image cytometer correlated well with those made using the flow cytometric assay to detect PMAs (R = 0.944) (Fig. 3).

Bottom Line: Moreover, the PMA measurements made by the ADAM(®) cytometer exhibited a high correlation with those made by a flow cytometric assay (R = 0.944).The ADAM(®) cytometer is a suitable alternative method to the flow cytometry-based assays.This, in turn, will provide valuable information regarding patient propensities to thrombotic diseases.

View Article: PubMed Central - PubMed

Affiliation: 1. Department of Laboratory Medicine, College of Medicine, Korea University Guro Hospital, Seoul 152-703, South Korea.

ABSTRACT

Background: Inappropriate platelet activation is known to be associated with various thrombotic disorders. Platelet-monocyte aggregates (PMAs), whose formation is mediated by platelet surface P-selectin (CD62P), can be used as a reliable marker to detect platelet activation. Previous studies have generally detected PMAs through flow cytometry-based approaches. Recently, the ADAM(®) image cytometer (Nanoentek Inc., Seoul, Korea) was developed for image-based cellular analysis. In this study, we detected PMAs with the ADAM(®) cytometer, evaluated the reproducibility of the measurements made by the ADAM(®) cytometer, and compared the abilities of the ADAM(®) cytometer and a flow cytometric assay to detect PMAs.

Methods: Whole blood samples were collected from patients. Within 5 minutes of collection, anticoagulated whole blood samples were fixed in 10% paraformaldehyde and 5% glyoxal. Nineteen clinical specimens were collected; each was analyzed three times with the ADAM(®) cytometer in order to assess the reproducibility of its measurements. To compare the ability of the ADAM(®) cytometer with that of a flow cytometer to detect PMAs, each cytometer was used for 23 clinical samples and the correlation of the measurements was determined.

Results: The PMA measurements made by the ADAM(®) cytometer showed good reproducibility (CV < 10% for all specimens). Moreover, the PMA measurements made by the ADAM(®) cytometer exhibited a high correlation with those made by a flow cytometric assay (R = 0.944).

Conclusions: The ADAM(®) cytometer is a suitable alternative method to the flow cytometry-based assays. Since the ADAM cytometer does not need specialized instrument knowledge or software proficiency (unlike flow cytometry), the ADAM(®) cytometer can be used as a rapid and reliable POCT device to measure platelet activation in peripheral blood. This, in turn, will provide valuable information regarding patient propensities to thrombotic diseases.

Show MeSH
Related in: MedlinePlus