Limits...
Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells.

Sheu SM, Cheng H, Kao CY, Yang YJ, Wu JJ, Sheu BS - J. Biomed. Sci. (2014)

Bottom Line: Hyperglycemia increases the risk of gastric cancer in H. pylori-infected patients.Furthermore, adhesion enhancement of H. pylori was significantly higher in 200 mg/dL of glucose as compared to that in 100 and 150 mg/dL (p < 0.05).The cell-associated CagA and phosphorylated-CagA was significantly increased in 150 and 200 mg/dL of glucose concentrations as compared to that of 100 mg/dL (p < 0.05), which were found to be dose-dependent.

View Article: PubMed Central - PubMed

Affiliation: Institute of Basic Medical Sciences, College of Medicine, National Cheng-Kung University, Tainan, Taiwan. sheubs@mail.ncku.edu.tw.

ABSTRACT

Background: Hyperglycemia increases the risk of gastric cancer in H. pylori-infected patients. High glucose could increase endothelial permeability and cancer-associated signaling. These suggest high glucose may affect H. pylori or its infected status.We used two strains to investigate whether H. pylori growth, viability, adhesion and CagA-phosphorylation level in the infected-AGS cells were influenced by glucose concentration (100, 150, and 200 mg/dL).

Results: The growth curves of both strains in 200 mg/dL of glucose were maintained at the highest optimal density after 48 h and the best viability of both strains were retained in the same glucose condition at 72 h. Furthermore, adhesion enhancement of H. pylori was significantly higher in 200 mg/dL of glucose as compared to that in 100 and 150 mg/dL (p < 0.05). CagA protein also increased in higher glucose condition. The cell-associated CagA and phosphorylated-CagA was significantly increased in 150 and 200 mg/dL of glucose concentrations as compared to that of 100 mg/dL (p < 0.05), which were found to be dose-dependent.

Conclusion: Higher glucose could maintain H. pylori growth and viability after 48 h. H. pylori adhesion and CagA increased to further facilitate the enhancement of cell-associated CagA and phosphorylated CagA in higher glucose conditions.

Show MeSH

Related in: MedlinePlus

Adhesion ability ofH. pyloriincreased under the treatment of higher glucose concentration. Strain J99 and 43504 growth in different level of glucose (100, 150, 200 mg/dL) was added to AGS cells with the pretreatment of the same glucose concentration (A, B) or the pretreatment of contrary concentration (200, 150, 100 mg/dL) (C, D). Relative adhesion ratio indicates that the adhesion of H. pylori in 100 mg/dL of glucose serve as the reference, and the bacterial adhesion value in 150 and 200 mg/dL of glucose was divided by the value of reference. *indicated a significant difference between the treatment of different glucose concentrations (paired t test, p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4196111&req=5

Fig2: Adhesion ability ofH. pyloriincreased under the treatment of higher glucose concentration. Strain J99 and 43504 growth in different level of glucose (100, 150, 200 mg/dL) was added to AGS cells with the pretreatment of the same glucose concentration (A, B) or the pretreatment of contrary concentration (200, 150, 100 mg/dL) (C, D). Relative adhesion ratio indicates that the adhesion of H. pylori in 100 mg/dL of glucose serve as the reference, and the bacterial adhesion value in 150 and 200 mg/dL of glucose was divided by the value of reference. *indicated a significant difference between the treatment of different glucose concentrations (paired t test, p < 0.05).

Mentions: With the treatment of 200 mg/dL of glucose to H. pylori and AGS cells, the adhesion ability of strain J99 and 43504 was significantly enhanced as compared with that of 150 and 100 mg/dL of glucose treatment (Figure 2A and B). In order to test whether cellular factors play some role in adhesion enhancement, strain J99 and 43504 growth in 100, 150, and 200 mg/dL of glucose adhered to AGS cells with pretreatment of with contrary concentration (200, 150, 100 mg/dL), respectively (shown in Figure 2C and D). Nevertheless, the adhesion abilities within each strain were similarly disclosed with an increasing trend based on the glucose level treated to the bacteria (strain J99 on Figure 2A and C; strain 43504 on Figure 2B and D).Figure 2


Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells.

Sheu SM, Cheng H, Kao CY, Yang YJ, Wu JJ, Sheu BS - J. Biomed. Sci. (2014)

Adhesion ability ofH. pyloriincreased under the treatment of higher glucose concentration. Strain J99 and 43504 growth in different level of glucose (100, 150, 200 mg/dL) was added to AGS cells with the pretreatment of the same glucose concentration (A, B) or the pretreatment of contrary concentration (200, 150, 100 mg/dL) (C, D). Relative adhesion ratio indicates that the adhesion of H. pylori in 100 mg/dL of glucose serve as the reference, and the bacterial adhesion value in 150 and 200 mg/dL of glucose was divided by the value of reference. *indicated a significant difference between the treatment of different glucose concentrations (paired t test, p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4196111&req=5

Fig2: Adhesion ability ofH. pyloriincreased under the treatment of higher glucose concentration. Strain J99 and 43504 growth in different level of glucose (100, 150, 200 mg/dL) was added to AGS cells with the pretreatment of the same glucose concentration (A, B) or the pretreatment of contrary concentration (200, 150, 100 mg/dL) (C, D). Relative adhesion ratio indicates that the adhesion of H. pylori in 100 mg/dL of glucose serve as the reference, and the bacterial adhesion value in 150 and 200 mg/dL of glucose was divided by the value of reference. *indicated a significant difference between the treatment of different glucose concentrations (paired t test, p < 0.05).
Mentions: With the treatment of 200 mg/dL of glucose to H. pylori and AGS cells, the adhesion ability of strain J99 and 43504 was significantly enhanced as compared with that of 150 and 100 mg/dL of glucose treatment (Figure 2A and B). In order to test whether cellular factors play some role in adhesion enhancement, strain J99 and 43504 growth in 100, 150, and 200 mg/dL of glucose adhered to AGS cells with pretreatment of with contrary concentration (200, 150, 100 mg/dL), respectively (shown in Figure 2C and D). Nevertheless, the adhesion abilities within each strain were similarly disclosed with an increasing trend based on the glucose level treated to the bacteria (strain J99 on Figure 2A and C; strain 43504 on Figure 2B and D).Figure 2

Bottom Line: Hyperglycemia increases the risk of gastric cancer in H. pylori-infected patients.Furthermore, adhesion enhancement of H. pylori was significantly higher in 200 mg/dL of glucose as compared to that in 100 and 150 mg/dL (p < 0.05).The cell-associated CagA and phosphorylated-CagA was significantly increased in 150 and 200 mg/dL of glucose concentrations as compared to that of 100 mg/dL (p < 0.05), which were found to be dose-dependent.

View Article: PubMed Central - PubMed

Affiliation: Institute of Basic Medical Sciences, College of Medicine, National Cheng-Kung University, Tainan, Taiwan. sheubs@mail.ncku.edu.tw.

ABSTRACT

Background: Hyperglycemia increases the risk of gastric cancer in H. pylori-infected patients. High glucose could increase endothelial permeability and cancer-associated signaling. These suggest high glucose may affect H. pylori or its infected status.We used two strains to investigate whether H. pylori growth, viability, adhesion and CagA-phosphorylation level in the infected-AGS cells were influenced by glucose concentration (100, 150, and 200 mg/dL).

Results: The growth curves of both strains in 200 mg/dL of glucose were maintained at the highest optimal density after 48 h and the best viability of both strains were retained in the same glucose condition at 72 h. Furthermore, adhesion enhancement of H. pylori was significantly higher in 200 mg/dL of glucose as compared to that in 100 and 150 mg/dL (p < 0.05). CagA protein also increased in higher glucose condition. The cell-associated CagA and phosphorylated-CagA was significantly increased in 150 and 200 mg/dL of glucose concentrations as compared to that of 100 mg/dL (p < 0.05), which were found to be dose-dependent.

Conclusion: Higher glucose could maintain H. pylori growth and viability after 48 h. H. pylori adhesion and CagA increased to further facilitate the enhancement of cell-associated CagA and phosphorylated CagA in higher glucose conditions.

Show MeSH
Related in: MedlinePlus