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Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells.

Sheu SM, Cheng H, Kao CY, Yang YJ, Wu JJ, Sheu BS - J. Biomed. Sci. (2014)

Bottom Line: Hyperglycemia increases the risk of gastric cancer in H. pylori-infected patients.The cell-associated CagA and phosphorylated-CagA was significantly increased in 150 and 200 mg/dL of glucose concentrations as compared to that of 100 mg/dL (p < 0.05), which were found to be dose-dependent.H. pylori adhesion and CagA increased to further facilitate the enhancement of cell-associated CagA and phosphorylated CagA in higher glucose conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Basic Medical Sciences, College of Medicine, National Cheng-Kung University, Tainan, Taiwan. sheubs@mail.ncku.edu.tw.

ABSTRACT

Background: Hyperglycemia increases the risk of gastric cancer in H. pylori-infected patients. High glucose could increase endothelial permeability and cancer-associated signaling. These suggest high glucose may affect H. pylori or its infected status.We used two strains to investigate whether H. pylori growth, viability, adhesion and CagA-phosphorylation level in the infected-AGS cells were influenced by glucose concentration (100, 150, and 200 mg/dL).

Results: The growth curves of both strains in 200 mg/dL of glucose were maintained at the highest optimal density after 48 h and the best viability of both strains were retained in the same glucose condition at 72 h. Furthermore, adhesion enhancement of H. pylori was significantly higher in 200 mg/dL of glucose as compared to that in 100 and 150 mg/dL (p < 0.05). CagA protein also increased in higher glucose condition. The cell-associated CagA and phosphorylated-CagA was significantly increased in 150 and 200 mg/dL of glucose concentrations as compared to that of 100 mg/dL (p < 0.05), which were found to be dose-dependent.

Conclusion: Higher glucose could maintain H. pylori growth and viability after 48 h. H. pylori adhesion and CagA increased to further facilitate the enhancement of cell-associated CagA and phosphorylated CagA in higher glucose conditions.

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H. pylorigrowth and viability was increased under higher glucose level after 48 hr. (A, B) Strain J99 and 43504 grew in Brucella broth containing 10% horse serum and different level of glucose (100, 150, 200 mg/dL). The optical density (OD) at 600 nm of bacterial growth was evaluated during 72 h. (C, D) Colony formation unit (CFU) of strain J99 and 43504 was determined at growth curve of 48 and 72 h. * indicated p < 0.05 (paired t test).
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Fig1: H. pylorigrowth and viability was increased under higher glucose level after 48 hr. (A, B) Strain J99 and 43504 grew in Brucella broth containing 10% horse serum and different level of glucose (100, 150, 200 mg/dL). The optical density (OD) at 600 nm of bacterial growth was evaluated during 72 h. (C, D) Colony formation unit (CFU) of strain J99 and 43504 was determined at growth curve of 48 and 72 h. * indicated p < 0.05 (paired t test).

Mentions: Strain J99 and 43504, grown in broth containing three concentrations of glucose (100, 150, 200 mg/dL), were applied to detect the growth curve during 72 h (Figure 1A and B). Optical density of strain J99 at 200 mg/dL were kept on increasing at 72 h, however, OD value was decreased in 100 and 150 mg/dL of glucose conditions at the same time (Figure 1A). The significant difference between 3 growth curves of strain J99 was observed at 48 h (J99-100 vs. J99-150, p < 0.05; J99-100 vs. J99-200, p < 0.05; J99-150 vs. J99-200, p > 0.05) and 72 h (J99-100 vs. J99-150, p = 0.05; J99-100 vs. J99-200, p < 0.05; J99-150 vs. J99-200, p < 0.05). Growth of strain 43504 in higher glucose concentrations (150 and 200 mg/dL) had the trend of higher optimal density, as compared to low glucose (100 mg/dL), except 48 h. Besides strain 43504 at 48 h, H. pylori viability was increased by the increasing glucose concentration (Figure 1C and D). Especially at 72 h, the viability of two strains cultured in 150 and 200 mg/dL were consistently increased as compared to that in 100 mg/dL (p < 0.05). Viability showed 3-4 fold increases in 200 mg/dL as compared to that in 150 mg/dL (p < 0.05).Figure 1


Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells.

Sheu SM, Cheng H, Kao CY, Yang YJ, Wu JJ, Sheu BS - J. Biomed. Sci. (2014)

H. pylorigrowth and viability was increased under higher glucose level after 48 hr. (A, B) Strain J99 and 43504 grew in Brucella broth containing 10% horse serum and different level of glucose (100, 150, 200 mg/dL). The optical density (OD) at 600 nm of bacterial growth was evaluated during 72 h. (C, D) Colony formation unit (CFU) of strain J99 and 43504 was determined at growth curve of 48 and 72 h. * indicated p < 0.05 (paired t test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4196111&req=5

Fig1: H. pylorigrowth and viability was increased under higher glucose level after 48 hr. (A, B) Strain J99 and 43504 grew in Brucella broth containing 10% horse serum and different level of glucose (100, 150, 200 mg/dL). The optical density (OD) at 600 nm of bacterial growth was evaluated during 72 h. (C, D) Colony formation unit (CFU) of strain J99 and 43504 was determined at growth curve of 48 and 72 h. * indicated p < 0.05 (paired t test).
Mentions: Strain J99 and 43504, grown in broth containing three concentrations of glucose (100, 150, 200 mg/dL), were applied to detect the growth curve during 72 h (Figure 1A and B). Optical density of strain J99 at 200 mg/dL were kept on increasing at 72 h, however, OD value was decreased in 100 and 150 mg/dL of glucose conditions at the same time (Figure 1A). The significant difference between 3 growth curves of strain J99 was observed at 48 h (J99-100 vs. J99-150, p < 0.05; J99-100 vs. J99-200, p < 0.05; J99-150 vs. J99-200, p > 0.05) and 72 h (J99-100 vs. J99-150, p = 0.05; J99-100 vs. J99-200, p < 0.05; J99-150 vs. J99-200, p < 0.05). Growth of strain 43504 in higher glucose concentrations (150 and 200 mg/dL) had the trend of higher optimal density, as compared to low glucose (100 mg/dL), except 48 h. Besides strain 43504 at 48 h, H. pylori viability was increased by the increasing glucose concentration (Figure 1C and D). Especially at 72 h, the viability of two strains cultured in 150 and 200 mg/dL were consistently increased as compared to that in 100 mg/dL (p < 0.05). Viability showed 3-4 fold increases in 200 mg/dL as compared to that in 150 mg/dL (p < 0.05).Figure 1

Bottom Line: Hyperglycemia increases the risk of gastric cancer in H. pylori-infected patients.The cell-associated CagA and phosphorylated-CagA was significantly increased in 150 and 200 mg/dL of glucose concentrations as compared to that of 100 mg/dL (p < 0.05), which were found to be dose-dependent.H. pylori adhesion and CagA increased to further facilitate the enhancement of cell-associated CagA and phosphorylated CagA in higher glucose conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Basic Medical Sciences, College of Medicine, National Cheng-Kung University, Tainan, Taiwan. sheubs@mail.ncku.edu.tw.

ABSTRACT

Background: Hyperglycemia increases the risk of gastric cancer in H. pylori-infected patients. High glucose could increase endothelial permeability and cancer-associated signaling. These suggest high glucose may affect H. pylori or its infected status.We used two strains to investigate whether H. pylori growth, viability, adhesion and CagA-phosphorylation level in the infected-AGS cells were influenced by glucose concentration (100, 150, and 200 mg/dL).

Results: The growth curves of both strains in 200 mg/dL of glucose were maintained at the highest optimal density after 48 h and the best viability of both strains were retained in the same glucose condition at 72 h. Furthermore, adhesion enhancement of H. pylori was significantly higher in 200 mg/dL of glucose as compared to that in 100 and 150 mg/dL (p < 0.05). CagA protein also increased in higher glucose condition. The cell-associated CagA and phosphorylated-CagA was significantly increased in 150 and 200 mg/dL of glucose concentrations as compared to that of 100 mg/dL (p < 0.05), which were found to be dose-dependent.

Conclusion: Higher glucose could maintain H. pylori growth and viability after 48 h. H. pylori adhesion and CagA increased to further facilitate the enhancement of cell-associated CagA and phosphorylated CagA in higher glucose conditions.

Show MeSH
Related in: MedlinePlus