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Transcriptome profile of OVCAR3 cisplatin-resistant ovarian cancer cell line

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Thus, OVCAR3 serves as a model cell line for drug resistance in ovarian cancer... Here, we perform a comparative transcriptome analysis from the US National Cancer Institute human tumor cell line anticancer drug screen (NCI60) dataset... Our results indicate a specific gene transcription profile of OVCAR3 genes relative to non-cancerous Human Ovarian Surface Epithelial cells (HOSE) and drug sensitive Serous Ovarian Cancer Epithelial Samples (CEPI) and SKOV3 cell lines... Transcriptome data analysis was conducted with Partek Genomics Suite version 6.6... The WEB-based GEne SeT AnaLysis Toolkit (WebGestalt) was used to perform enrichment analysis... Genes present in KEGG pathway, PharmGKB Disease, and Drug terms enrichment sets were connected and expanded to one degree of biological interaction using the Michigan Molecular Interactions databases plugin and visualized using Cytoscape version 2.8.3... Transcriptome analysis of OVCAR3 specific gene expression changes resulted in 160 significant transcripts with a fold change > ±2 and an ANOVA derived Benjamini Hochberg adjusted p-value < 0.001... Enrichment analysis using a Hypergeometric test identified 189 PharmGKB Disease terms, 90 Drug terms and 31 KEGG pathways associated with these genes... A union of the disease, drug and KEGG pathway gene lists yielded 14 common genes for the dataset which were unique to OVCAR3 cells versus SKOV3 and CEPI (Table 1)... This list of OVCAR3 unique genes, and the resulting interactions graph (Figure 1) represent potential pathways of drug resistance associated genes in ovarian cancer.

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Interaction network of OVCAR3 unique genes. Diamond and rectangle nodes are seed nodes of 14 OVCAR3 unique genes; circular nodes are 1 degree of biological interactions; rounded rectangular nodes are the highly connected hubs in the network (FYN and ERBB2).
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Figure 1: Interaction network of OVCAR3 unique genes. Diamond and rectangle nodes are seed nodes of 14 OVCAR3 unique genes; circular nodes are 1 degree of biological interactions; rounded rectangular nodes are the highly connected hubs in the network (FYN and ERBB2).

Mentions: This list of OVCAR3 unique genes, and the resulting interactions graph (Figure 1) represent potential pathways of drug resistance associated genes in ovarian cancer. Notably, ERBB2 (HER2) and FYN are the hub genes of the interaction network specific for OVCAR3 cell line. Thus, they may provide valuable insights into the drug resistance etiology of ovarian cancer. ERBB2 (HER2) has previously been reported to interact with Estrogen Receptor (ESR2) in Breast Cancer [8] and FYN has been implicated in Glioblastoma and T-cell Lymphomas [9,10]; however their detailed roles in Ovarian Cancer have only recently been studied, warranting further investigation [11,12].


Transcriptome profile of OVCAR3 cisplatin-resistant ovarian cancer cell line
Interaction network of OVCAR3 unique genes. Diamond and rectangle nodes are seed nodes of 14 OVCAR3 unique genes; circular nodes are 1 degree of biological interactions; rounded rectangular nodes are the highly connected hubs in the network (FYN and ERBB2).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4196045&req=5

Figure 1: Interaction network of OVCAR3 unique genes. Diamond and rectangle nodes are seed nodes of 14 OVCAR3 unique genes; circular nodes are 1 degree of biological interactions; rounded rectangular nodes are the highly connected hubs in the network (FYN and ERBB2).
Mentions: This list of OVCAR3 unique genes, and the resulting interactions graph (Figure 1) represent potential pathways of drug resistance associated genes in ovarian cancer. Notably, ERBB2 (HER2) and FYN are the hub genes of the interaction network specific for OVCAR3 cell line. Thus, they may provide valuable insights into the drug resistance etiology of ovarian cancer. ERBB2 (HER2) has previously been reported to interact with Estrogen Receptor (ESR2) in Breast Cancer [8] and FYN has been implicated in Glioblastoma and T-cell Lymphomas [9,10]; however their detailed roles in Ovarian Cancer have only recently been studied, warranting further investigation [11,12].

View Article: PubMed Central - HTML

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Thus, OVCAR3 serves as a model cell line for drug resistance in ovarian cancer... Here, we perform a comparative transcriptome analysis from the US National Cancer Institute human tumor cell line anticancer drug screen (NCI60) dataset... Our results indicate a specific gene transcription profile of OVCAR3 genes relative to non-cancerous Human Ovarian Surface Epithelial cells (HOSE) and drug sensitive Serous Ovarian Cancer Epithelial Samples (CEPI) and SKOV3 cell lines... Transcriptome data analysis was conducted with Partek Genomics Suite version 6.6... The WEB-based GEne SeT AnaLysis Toolkit (WebGestalt) was used to perform enrichment analysis... Genes present in KEGG pathway, PharmGKB Disease, and Drug terms enrichment sets were connected and expanded to one degree of biological interaction using the Michigan Molecular Interactions databases plugin and visualized using Cytoscape version 2.8.3... Transcriptome analysis of OVCAR3 specific gene expression changes resulted in 160 significant transcripts with a fold change > ±2 and an ANOVA derived Benjamini Hochberg adjusted p-value < 0.001... Enrichment analysis using a Hypergeometric test identified 189 PharmGKB Disease terms, 90 Drug terms and 31 KEGG pathways associated with these genes... A union of the disease, drug and KEGG pathway gene lists yielded 14 common genes for the dataset which were unique to OVCAR3 cells versus SKOV3 and CEPI (Table 1)... This list of OVCAR3 unique genes, and the resulting interactions graph (Figure 1) represent potential pathways of drug resistance associated genes in ovarian cancer.

No MeSH data available.