Limits...
Cardiac troponin I but not cardiac troponin T adheres to polysulfone dialyser membranes in an in vitro haemodialysis model: explanation for lower serum cTnI concentrations following dialysis.

Gaze DC, Collinson PO - Open Heart (2014)

Bottom Line: Serum cTnI was significantly lower (p=0.043) following a session of HD whereas cTnT concentrations were similar to those obtained before HD.The adherence of cTnI to the dialyser membrane is responsible for the observed decrease in serum cTnI following a session of dialysis.The adherence of cTnT or T-I-C complex to the dialyser membrane could not be demonstrated and supports the observation that pre-HD and post-HD serum concentrations of cTnT are similar.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Pathology , Clinical Blood Sciences, St George's Hospital & Medical School , London , UK.

ABSTRACT

Background: Elevated serum cardiac troponin T (cTnT) and I (cTnI) can occur in patients with chronic kidney disease. Differences in cTn concentrations between cTnT and cTnI have been reported but the mechanism of such discrepancy has not been investigated. This study investigates the clearance of cTn with the aid of an in vitro model of haemodialysis (HD).

Methods: Serum was obtained before and after a single session of dialysis from 53 patients receiving HD and assayed for cTnT and cTnI. An in vitro model of the dialysis process was used to investigate the mechanism of clearance of cTn during HD.

Results: Serum cTnI was significantly lower (p=0.043) following a session of HD whereas cTnT concentrations were similar to those obtained before HD. Using an in vitro model of dialysis, it was demonstrated that cTnI is not dialysed from the vascular compartment but adheres to the dialyser membrane.

Conclusions: The adherence of cTnI to the dialyser membrane is responsible for the observed decrease in serum cTnI following a session of dialysis. The adherence of cTnT or T-I-C complex to the dialyser membrane could not be demonstrated and supports the observation that pre-HD and post-HD serum concentrations of cTnT are similar.

No MeSH data available.


Related in: MedlinePlus

Immunofluorescence of polysulfone haemodialysis membranes. Series A1–E1: immunofluorescent images; series A2–E2, corresponding standard bright field light microscopy images. (A) Troponin (Tn) free serum pool; (B) free cardiac Tn I (cTnI) spiked pool; (C) free cTnT spiked pool; (D) I-C binary complex spiked pool; (E) T-I-C complex spiked pool (all images are transverse sections at ×40 magnification).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4195923&req=5

OPENHRT2014000108F4: Immunofluorescence of polysulfone haemodialysis membranes. Series A1–E1: immunofluorescent images; series A2–E2, corresponding standard bright field light microscopy images. (A) Troponin (Tn) free serum pool; (B) free cardiac Tn I (cTnI) spiked pool; (C) free cTnT spiked pool; (D) I-C binary complex spiked pool; (E) T-I-C complex spiked pool (all images are transverse sections at ×40 magnification).

Mentions: After dismantling the dialyser membrane and exposure of the cellulose acetate core, it can be demonstrated that cTn-free serum does not produce any fluorescent signal (figure 4A1). To demonstrate that the TS sections of membrane had adequately adhered to the Silane-Prep slide, the corresponding light micrograph is shown in figure 4A2.


Cardiac troponin I but not cardiac troponin T adheres to polysulfone dialyser membranes in an in vitro haemodialysis model: explanation for lower serum cTnI concentrations following dialysis.

Gaze DC, Collinson PO - Open Heart (2014)

Immunofluorescence of polysulfone haemodialysis membranes. Series A1–E1: immunofluorescent images; series A2–E2, corresponding standard bright field light microscopy images. (A) Troponin (Tn) free serum pool; (B) free cardiac Tn I (cTnI) spiked pool; (C) free cTnT spiked pool; (D) I-C binary complex spiked pool; (E) T-I-C complex spiked pool (all images are transverse sections at ×40 magnification).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4195923&req=5

OPENHRT2014000108F4: Immunofluorescence of polysulfone haemodialysis membranes. Series A1–E1: immunofluorescent images; series A2–E2, corresponding standard bright field light microscopy images. (A) Troponin (Tn) free serum pool; (B) free cardiac Tn I (cTnI) spiked pool; (C) free cTnT spiked pool; (D) I-C binary complex spiked pool; (E) T-I-C complex spiked pool (all images are transverse sections at ×40 magnification).
Mentions: After dismantling the dialyser membrane and exposure of the cellulose acetate core, it can be demonstrated that cTn-free serum does not produce any fluorescent signal (figure 4A1). To demonstrate that the TS sections of membrane had adequately adhered to the Silane-Prep slide, the corresponding light micrograph is shown in figure 4A2.

Bottom Line: Serum cTnI was significantly lower (p=0.043) following a session of HD whereas cTnT concentrations were similar to those obtained before HD.The adherence of cTnI to the dialyser membrane is responsible for the observed decrease in serum cTnI following a session of dialysis.The adherence of cTnT or T-I-C complex to the dialyser membrane could not be demonstrated and supports the observation that pre-HD and post-HD serum concentrations of cTnT are similar.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Pathology , Clinical Blood Sciences, St George's Hospital & Medical School , London , UK.

ABSTRACT

Background: Elevated serum cardiac troponin T (cTnT) and I (cTnI) can occur in patients with chronic kidney disease. Differences in cTn concentrations between cTnT and cTnI have been reported but the mechanism of such discrepancy has not been investigated. This study investigates the clearance of cTn with the aid of an in vitro model of haemodialysis (HD).

Methods: Serum was obtained before and after a single session of dialysis from 53 patients receiving HD and assayed for cTnT and cTnI. An in vitro model of the dialysis process was used to investigate the mechanism of clearance of cTn during HD.

Results: Serum cTnI was significantly lower (p=0.043) following a session of HD whereas cTnT concentrations were similar to those obtained before HD. Using an in vitro model of dialysis, it was demonstrated that cTnI is not dialysed from the vascular compartment but adheres to the dialyser membrane.

Conclusions: The adherence of cTnI to the dialyser membrane is responsible for the observed decrease in serum cTnI following a session of dialysis. The adherence of cTnT or T-I-C complex to the dialyser membrane could not be demonstrated and supports the observation that pre-HD and post-HD serum concentrations of cTnT are similar.

No MeSH data available.


Related in: MedlinePlus