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Cardiac troponin I but not cardiac troponin T adheres to polysulfone dialyser membranes in an in vitro haemodialysis model: explanation for lower serum cTnI concentrations following dialysis.

Gaze DC, Collinson PO - Open Heart (2014)

Bottom Line: Serum cTnI was significantly lower (p=0.043) following a session of HD whereas cTnT concentrations were similar to those obtained before HD.The adherence of cTnI to the dialyser membrane is responsible for the observed decrease in serum cTnI following a session of dialysis.The adherence of cTnT or T-I-C complex to the dialyser membrane could not be demonstrated and supports the observation that pre-HD and post-HD serum concentrations of cTnT are similar.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Pathology , Clinical Blood Sciences, St George's Hospital & Medical School , London , UK.

ABSTRACT

Background: Elevated serum cardiac troponin T (cTnT) and I (cTnI) can occur in patients with chronic kidney disease. Differences in cTn concentrations between cTnT and cTnI have been reported but the mechanism of such discrepancy has not been investigated. This study investigates the clearance of cTn with the aid of an in vitro model of haemodialysis (HD).

Methods: Serum was obtained before and after a single session of dialysis from 53 patients receiving HD and assayed for cTnT and cTnI. An in vitro model of the dialysis process was used to investigate the mechanism of clearance of cTn during HD.

Results: Serum cTnI was significantly lower (p=0.043) following a session of HD whereas cTnT concentrations were similar to those obtained before HD. Using an in vitro model of dialysis, it was demonstrated that cTnI is not dialysed from the vascular compartment but adheres to the dialyser membrane.

Conclusions: The adherence of cTnI to the dialyser membrane is responsible for the observed decrease in serum cTnI following a session of dialysis. The adherence of cTnT or T-I-C complex to the dialyser membrane could not be demonstrated and supports the observation that pre-HD and post-HD serum concentrations of cTnT are similar.

No MeSH data available.


Related in: MedlinePlus

Immunofluorescence of polysulfone dialysis membranes. (A) Transverse section of membrane tube incubated with free cardiac troponin I (cTnI) and stained with anti-cTnI MAb. (B) Longitudinal section of membrane tube incubated with free cTnI and stained with anti-cTnI MAb. (C) Transverse section of multiple tubes incubated with free cTnT and stained with anti-cTnI M7 MAb. (D) Transverse section of a single polysulfone tube incubated with Tn T-I-C binary complex incubated with anti-cTnI and anti-cTnT MAb (×100 magnification).
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OPENHRT2014000108F3: Immunofluorescence of polysulfone dialysis membranes. (A) Transverse section of membrane tube incubated with free cardiac troponin I (cTnI) and stained with anti-cTnI MAb. (B) Longitudinal section of membrane tube incubated with free cTnI and stained with anti-cTnI MAb. (C) Transverse section of multiple tubes incubated with free cTnT and stained with anti-cTnI M7 MAb. (D) Transverse section of a single polysulfone tube incubated with Tn T-I-C binary complex incubated with anti-cTnI and anti-cTnT MAb (×100 magnification).

Mentions: Using cut cellulose membranes and simple passive diffusion following incubation of the polysulfone membrane in an Eppendorf tube with very high concentrations of serum containing cTnT, cTnI and T-I-C tertiary complex, immunofluorescent signal can be seen within the lumen in the TS (figure 3A) and LS sections of the membrane (figure 3B) incubated with free cTnI. There was a lack of immunofluorescent signal when membrane was incubated with either free cTnT (figure 3C) or T-I-C binary complex (figure 3D). Autofluorescence of the fleece fibre packing material occurs (figure 3C marked with*) which adsorbs the streptavidin-labelled FITC and is an artefact.


Cardiac troponin I but not cardiac troponin T adheres to polysulfone dialyser membranes in an in vitro haemodialysis model: explanation for lower serum cTnI concentrations following dialysis.

Gaze DC, Collinson PO - Open Heart (2014)

Immunofluorescence of polysulfone dialysis membranes. (A) Transverse section of membrane tube incubated with free cardiac troponin I (cTnI) and stained with anti-cTnI MAb. (B) Longitudinal section of membrane tube incubated with free cTnI and stained with anti-cTnI MAb. (C) Transverse section of multiple tubes incubated with free cTnT and stained with anti-cTnI M7 MAb. (D) Transverse section of a single polysulfone tube incubated with Tn T-I-C binary complex incubated with anti-cTnI and anti-cTnT MAb (×100 magnification).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4195923&req=5

OPENHRT2014000108F3: Immunofluorescence of polysulfone dialysis membranes. (A) Transverse section of membrane tube incubated with free cardiac troponin I (cTnI) and stained with anti-cTnI MAb. (B) Longitudinal section of membrane tube incubated with free cTnI and stained with anti-cTnI MAb. (C) Transverse section of multiple tubes incubated with free cTnT and stained with anti-cTnI M7 MAb. (D) Transverse section of a single polysulfone tube incubated with Tn T-I-C binary complex incubated with anti-cTnI and anti-cTnT MAb (×100 magnification).
Mentions: Using cut cellulose membranes and simple passive diffusion following incubation of the polysulfone membrane in an Eppendorf tube with very high concentrations of serum containing cTnT, cTnI and T-I-C tertiary complex, immunofluorescent signal can be seen within the lumen in the TS (figure 3A) and LS sections of the membrane (figure 3B) incubated with free cTnI. There was a lack of immunofluorescent signal when membrane was incubated with either free cTnT (figure 3C) or T-I-C binary complex (figure 3D). Autofluorescence of the fleece fibre packing material occurs (figure 3C marked with*) which adsorbs the streptavidin-labelled FITC and is an artefact.

Bottom Line: Serum cTnI was significantly lower (p=0.043) following a session of HD whereas cTnT concentrations were similar to those obtained before HD.The adherence of cTnI to the dialyser membrane is responsible for the observed decrease in serum cTnI following a session of dialysis.The adherence of cTnT or T-I-C complex to the dialyser membrane could not be demonstrated and supports the observation that pre-HD and post-HD serum concentrations of cTnT are similar.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Pathology , Clinical Blood Sciences, St George's Hospital & Medical School , London , UK.

ABSTRACT

Background: Elevated serum cardiac troponin T (cTnT) and I (cTnI) can occur in patients with chronic kidney disease. Differences in cTn concentrations between cTnT and cTnI have been reported but the mechanism of such discrepancy has not been investigated. This study investigates the clearance of cTn with the aid of an in vitro model of haemodialysis (HD).

Methods: Serum was obtained before and after a single session of dialysis from 53 patients receiving HD and assayed for cTnT and cTnI. An in vitro model of the dialysis process was used to investigate the mechanism of clearance of cTn during HD.

Results: Serum cTnI was significantly lower (p=0.043) following a session of HD whereas cTnT concentrations were similar to those obtained before HD. Using an in vitro model of dialysis, it was demonstrated that cTnI is not dialysed from the vascular compartment but adheres to the dialyser membrane.

Conclusions: The adherence of cTnI to the dialyser membrane is responsible for the observed decrease in serum cTnI following a session of dialysis. The adherence of cTnT or T-I-C complex to the dialyser membrane could not be demonstrated and supports the observation that pre-HD and post-HD serum concentrations of cTnT are similar.

No MeSH data available.


Related in: MedlinePlus