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Study of the association of adrenomedullin and basic-fibroblast growth factors with the peripheral arterial blood flow and endothelial dysfunction biomarkers in type 2 diabetic patients with peripheral vascular insufficiency.

Alrouq FA, Al-Masri AA, Al-Dokhi LM, Alregaiey KA, Bayoumy NM, Zakareia FA - J. Biomed. Sci. (2014)

Bottom Line: There was a significant increase in plasma AM, VCAM-1and ICAM-1, while a significant decrease in plasma b-FGF in diabetic patients with PVD (p < 0.05).This study elucidates for the first time that AM and b-FGF are correlated and have a direct impact on the peripheral blood flow, the rise of AM in diabetic PVD may be a consecutive and compensatory vasculo-protective effect as its angiogenic and anti-inflammatory properties act to relief the endothelial insult.It is not clear if the rise of AM and the decline of b- FGF levels may be consequences or predisposing factors for VCAM-1 and ICAM-1 elevation as these endothelial dysfunction biomarkers could reduce peripheral blood flow and vascular integrity.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Progressive micro-vascular vaso-degeneration is the major factor in progression of diabetic complications. Adrenomedullin (AM) and basic-Fibroblast growth factor (b-FGF) are strongly correlated with angiogenesis in vascular diseases. This study aims to provide base line data regarding the vascular effects and correlation of AM, and b-FGF with the peripheral blood flow in diabetic patients with peripheral vascular disease (PVD), and their effect on endothelial dysfunction markers. Ninety age- and sex matched females were enrolled in the study: 30 were controls, 30 had diabetes without complications (group II) and 30 had diabetes with PVD (group III) diagnosed by ankle/ brachial index (A/BI). Plasma levels of AM, b-FGF, intercellular adhesion molecule -1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were measured by indirect enzyme immunoassay (ELISA).

Results: There was a significant increase in plasma AM, VCAM-1and ICAM-1, while a significant decrease in plasma b-FGF in diabetic patients with PVD (p < 0.05). A positive correlation was observed between plasma AM, b-FGF and A/BI and a negative correlation with VCAM -1 and ICAM in diabetic PVD. AM was not a predictor, while b-FG, VCAM-1 and ICAM-1 could be predictors for peripheral blood flow in diabetic PVD.

Conclusion: This study elucidates for the first time that AM and b-FGF are correlated and have a direct impact on the peripheral blood flow, the rise of AM in diabetic PVD may be a consecutive and compensatory vasculo-protective effect as its angiogenic and anti-inflammatory properties act to relief the endothelial insult. Down expression of b-FGF may be a predisposing factor for micro-vascular derangement. It is not clear if the rise of AM and the decline of b- FGF levels may be consequences or predisposing factors for VCAM-1 and ICAM-1 elevation as these endothelial dysfunction biomarkers could reduce peripheral blood flow and vascular integrity. It is optimistic to believe that drug intervention through AM and b-FGF administration together with reversing the endothelial inflammatory process by targeting VCAM and ICAM could reduce the prevalence of diabetic vascular complications, reduce the risk of cerebrovascular and cardiovascular morbidity in diabetes through normalizing vascular endothelium function and peripheral blood flow.

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Plasma ICAM in all study groups. a. Control b. Diabetics without complications c. Diabetics with vasculopathy.
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Fig3: Plasma ICAM in all study groups. a. Control b. Diabetics without complications c. Diabetics with vasculopathy.

Mentions: There was significant increase of plasma AM, VCAM-1, and ICAM-1 in diabetic patients with PVD compared to both control and diabetics without complications. AM was 5.66 ± 1.31 in group III vs 2.96 ± 0.6 and 3.56 ± 0.5 in groups I and II respectively. (p < 0.05). Concerning plasma ICAM-1 it was 550.3 ± 15.4 in group III vs 318.66 ± 35.19 and 349.87 ± 27.18 in groups I and II respectively (p < 0.05). Concerning plasma VCAM-1, it was 863.59 ± 32.37 in group III vs 677.85 ± 17.63 and 690.64 ± 15.081 in groups I and II respectively. (p < 0.05). Concerning Plasma b-FGF levels they were significantly decreased in diabetic patients with PVD compared to both control and diabetics without complications. b-FGF was 1.66 ± 0.14 in group III vs 3.96 ± 0.6 and 3.56 ± 0.5 in groups I and II respectively (p < 0.05). (Table 2, Figures 1, 2, 3).Figure 1


Study of the association of adrenomedullin and basic-fibroblast growth factors with the peripheral arterial blood flow and endothelial dysfunction biomarkers in type 2 diabetic patients with peripheral vascular insufficiency.

Alrouq FA, Al-Masri AA, Al-Dokhi LM, Alregaiey KA, Bayoumy NM, Zakareia FA - J. Biomed. Sci. (2014)

Plasma ICAM in all study groups. a. Control b. Diabetics without complications c. Diabetics with vasculopathy.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4195904&req=5

Fig3: Plasma ICAM in all study groups. a. Control b. Diabetics without complications c. Diabetics with vasculopathy.
Mentions: There was significant increase of plasma AM, VCAM-1, and ICAM-1 in diabetic patients with PVD compared to both control and diabetics without complications. AM was 5.66 ± 1.31 in group III vs 2.96 ± 0.6 and 3.56 ± 0.5 in groups I and II respectively. (p < 0.05). Concerning plasma ICAM-1 it was 550.3 ± 15.4 in group III vs 318.66 ± 35.19 and 349.87 ± 27.18 in groups I and II respectively (p < 0.05). Concerning plasma VCAM-1, it was 863.59 ± 32.37 in group III vs 677.85 ± 17.63 and 690.64 ± 15.081 in groups I and II respectively. (p < 0.05). Concerning Plasma b-FGF levels they were significantly decreased in diabetic patients with PVD compared to both control and diabetics without complications. b-FGF was 1.66 ± 0.14 in group III vs 3.96 ± 0.6 and 3.56 ± 0.5 in groups I and II respectively (p < 0.05). (Table 2, Figures 1, 2, 3).Figure 1

Bottom Line: There was a significant increase in plasma AM, VCAM-1and ICAM-1, while a significant decrease in plasma b-FGF in diabetic patients with PVD (p < 0.05).This study elucidates for the first time that AM and b-FGF are correlated and have a direct impact on the peripheral blood flow, the rise of AM in diabetic PVD may be a consecutive and compensatory vasculo-protective effect as its angiogenic and anti-inflammatory properties act to relief the endothelial insult.It is not clear if the rise of AM and the decline of b- FGF levels may be consequences or predisposing factors for VCAM-1 and ICAM-1 elevation as these endothelial dysfunction biomarkers could reduce peripheral blood flow and vascular integrity.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Progressive micro-vascular vaso-degeneration is the major factor in progression of diabetic complications. Adrenomedullin (AM) and basic-Fibroblast growth factor (b-FGF) are strongly correlated with angiogenesis in vascular diseases. This study aims to provide base line data regarding the vascular effects and correlation of AM, and b-FGF with the peripheral blood flow in diabetic patients with peripheral vascular disease (PVD), and their effect on endothelial dysfunction markers. Ninety age- and sex matched females were enrolled in the study: 30 were controls, 30 had diabetes without complications (group II) and 30 had diabetes with PVD (group III) diagnosed by ankle/ brachial index (A/BI). Plasma levels of AM, b-FGF, intercellular adhesion molecule -1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were measured by indirect enzyme immunoassay (ELISA).

Results: There was a significant increase in plasma AM, VCAM-1and ICAM-1, while a significant decrease in plasma b-FGF in diabetic patients with PVD (p < 0.05). A positive correlation was observed between plasma AM, b-FGF and A/BI and a negative correlation with VCAM -1 and ICAM in diabetic PVD. AM was not a predictor, while b-FG, VCAM-1 and ICAM-1 could be predictors for peripheral blood flow in diabetic PVD.

Conclusion: This study elucidates for the first time that AM and b-FGF are correlated and have a direct impact on the peripheral blood flow, the rise of AM in diabetic PVD may be a consecutive and compensatory vasculo-protective effect as its angiogenic and anti-inflammatory properties act to relief the endothelial insult. Down expression of b-FGF may be a predisposing factor for micro-vascular derangement. It is not clear if the rise of AM and the decline of b- FGF levels may be consequences or predisposing factors for VCAM-1 and ICAM-1 elevation as these endothelial dysfunction biomarkers could reduce peripheral blood flow and vascular integrity. It is optimistic to believe that drug intervention through AM and b-FGF administration together with reversing the endothelial inflammatory process by targeting VCAM and ICAM could reduce the prevalence of diabetic vascular complications, reduce the risk of cerebrovascular and cardiovascular morbidity in diabetes through normalizing vascular endothelium function and peripheral blood flow.

Show MeSH
Related in: MedlinePlus