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Pathogenicity and tissue distribution of grass carp reovirus after intraperitoneal administration.

Liang HR, Li YG, Zeng WW, Wang YY, Wang Q, Wu SQ - Virol. J. (2014)

Bottom Line: The study showed a distribution of GCRV in different tissues and organs, particularly in the liver, spleen, kidney, intestine, and muscle, which had a higher number of viral RNA copies during the sixth to ninth days.The kidney had the highest numbers of viral RNA copies, as high as 24000 copies.Until the fourteenth day, nearly no viral RNA copies could be detected.

View Article: PubMed Central - PubMed

Affiliation: Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development,Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou 510380, China. sunny_929@163.com.

ABSTRACT
Grass carp reovirus (GCRV) is the causative agent of grass carp hemorrhage and causes significant loss of fingerlings. However, little is known about how the virus is distributed in organs and tissues. The aim of the present study was to investigate the distribution of different GCRV stains in tissues and organs of grass carp. The pathogenicity and tissue distribution of GCRV were monitored after intraperitoneal administration. The study showed a distribution of GCRV in different tissues and organs, particularly in the liver, spleen, kidney, intestine, and muscle, which had a higher number of viral RNA copies during the sixth to ninth days. The kidney had the highest numbers of viral RNA copies, as high as 24000 copies. Until the fourteenth day, nearly no viral RNA copies could be detected. This study defined the virus distribution in different tissues of grass carp inoculated by i.p. and supplied clues for the pathogenesis of GCRV.

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The viral RNA copy numbers of the muscle detected by real-time qPCR.
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Fig8: The viral RNA copy numbers of the muscle detected by real-time qPCR.

Mentions: To determine the viral RNA copy numbers in the muscle, muscle samples were analyzed by real-time qPCR (FigureĀ 8). The results showed that GCRV-CL had a higher number of virus from the sixth to tenth day and the highest number on the seventh day. GCRV-HZ08 had a higher number of virus in the seventh day.Figure 8


Pathogenicity and tissue distribution of grass carp reovirus after intraperitoneal administration.

Liang HR, Li YG, Zeng WW, Wang YY, Wang Q, Wu SQ - Virol. J. (2014)

The viral RNA copy numbers of the muscle detected by real-time qPCR.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4195869&req=5

Fig8: The viral RNA copy numbers of the muscle detected by real-time qPCR.
Mentions: To determine the viral RNA copy numbers in the muscle, muscle samples were analyzed by real-time qPCR (FigureĀ 8). The results showed that GCRV-CL had a higher number of virus from the sixth to tenth day and the highest number on the seventh day. GCRV-HZ08 had a higher number of virus in the seventh day.Figure 8

Bottom Line: The study showed a distribution of GCRV in different tissues and organs, particularly in the liver, spleen, kidney, intestine, and muscle, which had a higher number of viral RNA copies during the sixth to ninth days.The kidney had the highest numbers of viral RNA copies, as high as 24000 copies.Until the fourteenth day, nearly no viral RNA copies could be detected.

View Article: PubMed Central - PubMed

Affiliation: Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development,Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou 510380, China. sunny_929@163.com.

ABSTRACT
Grass carp reovirus (GCRV) is the causative agent of grass carp hemorrhage and causes significant loss of fingerlings. However, little is known about how the virus is distributed in organs and tissues. The aim of the present study was to investigate the distribution of different GCRV stains in tissues and organs of grass carp. The pathogenicity and tissue distribution of GCRV were monitored after intraperitoneal administration. The study showed a distribution of GCRV in different tissues and organs, particularly in the liver, spleen, kidney, intestine, and muscle, which had a higher number of viral RNA copies during the sixth to ninth days. The kidney had the highest numbers of viral RNA copies, as high as 24000 copies. Until the fourteenth day, nearly no viral RNA copies could be detected. This study defined the virus distribution in different tissues of grass carp inoculated by i.p. and supplied clues for the pathogenesis of GCRV.

Show MeSH
Related in: MedlinePlus