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Staphylococcus aureus from 152 cases of bovine, ovine and caprine mastitis investigated by Multiple-locus variable number of tandem repeat analysis (MLVA).

Bergonier D, Sobral D, Feßler AT, Jacquet E, Gilbert FB, Schwarz S, Treilles M, Bouloc P, Pourcel C, Vergnaud G - Vet. Res. (2014)

Bottom Line: This corresponds to a discriminatory index (D) value of 0.9936.Some of them displayed a relatively low level of diversity in agreement with a restricted ecological niche.These findings provide arguments suggesting that specific S. aureus lineages particularly adapted to ruminant mammary glands have emerged and that MLVA is a convenient tool to provide a broad overview of the population, owing to the availability via internet of databases compiling published MLVA genotypes.

View Article: PubMed Central - PubMed

Affiliation: INRA, UMR1225, IHAP, 31076, Toulouse, France. d.bergonier@envt.fr.

ABSTRACT
Staphylococcus aureus is one of the main etiological agents of mastitis in ruminants. In the present retrospective study, we evaluated the potential interest of a previously described automated multiple loci Variable Number of Tandem Repeats (VNTR) Assay (MLVA) comprising 16 loci as a first line tool to investigate the population structure of S. aureus from mastitis. We determined the genetic diversity of S. aureus strains from cases of clinical and subclinical mastitis in dairy cattle (n = 118, of which 16 were methicillin-resistant), sheep (n = 18) and goats (n = 16). The 152 strains could be subdivided into 115 MLVA genotypes (including 14 genotypes for the ovine strains and 15 genotypes for the caprine strains). This corresponds to a discriminatory index (D) value of 0.9936. Comparison with published MLVA data obtained using the same protocol applied to strains from diverse human and animal origins revealed a low number (8.5%) of human-related MLVA genotypes among the present collection. Eighteen percent of the S. aureus mastitis collection belonged to clonal complexes apparently not associated with other pathological conditions. Some of them displayed a relatively low level of diversity in agreement with a restricted ecological niche. These findings provide arguments suggesting that specific S. aureus lineages particularly adapted to ruminant mammary glands have emerged and that MLVA is a convenient tool to provide a broad overview of the population, owing to the availability via internet of databases compiling published MLVA genotypes.

No MeSH data available.


Related in: MedlinePlus

Minimum spanning tree of the 152S. aureusstrains using MLVA16Orsay. Each circle represents an MLVA genotype. The genotypes are coloured according to their host. Major CC are indicated.
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Fig1: Minimum spanning tree of the 152S. aureusstrains using MLVA16Orsay. Each circle represents an MLVA genotype. The genotypes are coloured according to their host. Major CC are indicated.

Mentions: The 152 ruminant strains were resolved into 115 MLVA genotypes with an overall diversity index of 0.9936. The 118 bovine, 18 ovine and 16 caprine strains belonged to 86, 14 and 15 MLVA genotypes, respectively. One hundred and forty strains fell into twelve clusters, nine of which comprising more than three strains (Figure 1). The clusters were assigned to known MLST-defined CC by comparison with previous MLVA data, spa typing and MLST analysis of selected strains. A dendrogram deduced from the clustering of the 152 S. aureus mastitis-associated strains is presented in Figure 2. CC97 and CC133 accounted for 22% (34 strains) and 21% (32 strains) of the studied collection, respectively. CC1, CC9, CC20, CC130, CC151, CC398 and CC479 together represented another 45% of the strains. The 16 ST398 MRSA strains were distributed into eight MLVA genotypes and three spa types. CC8 and CC30, frequently associated with human S. aureus infections, and CC425 a common ovine genotype (Figure 1), were represented by two strains each.Figure 1


Staphylococcus aureus from 152 cases of bovine, ovine and caprine mastitis investigated by Multiple-locus variable number of tandem repeat analysis (MLVA).

Bergonier D, Sobral D, Feßler AT, Jacquet E, Gilbert FB, Schwarz S, Treilles M, Bouloc P, Pourcel C, Vergnaud G - Vet. Res. (2014)

Minimum spanning tree of the 152S. aureusstrains using MLVA16Orsay. Each circle represents an MLVA genotype. The genotypes are coloured according to their host. Major CC are indicated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4195859&req=5

Fig1: Minimum spanning tree of the 152S. aureusstrains using MLVA16Orsay. Each circle represents an MLVA genotype. The genotypes are coloured according to their host. Major CC are indicated.
Mentions: The 152 ruminant strains were resolved into 115 MLVA genotypes with an overall diversity index of 0.9936. The 118 bovine, 18 ovine and 16 caprine strains belonged to 86, 14 and 15 MLVA genotypes, respectively. One hundred and forty strains fell into twelve clusters, nine of which comprising more than three strains (Figure 1). The clusters were assigned to known MLST-defined CC by comparison with previous MLVA data, spa typing and MLST analysis of selected strains. A dendrogram deduced from the clustering of the 152 S. aureus mastitis-associated strains is presented in Figure 2. CC97 and CC133 accounted for 22% (34 strains) and 21% (32 strains) of the studied collection, respectively. CC1, CC9, CC20, CC130, CC151, CC398 and CC479 together represented another 45% of the strains. The 16 ST398 MRSA strains were distributed into eight MLVA genotypes and three spa types. CC8 and CC30, frequently associated with human S. aureus infections, and CC425 a common ovine genotype (Figure 1), were represented by two strains each.Figure 1

Bottom Line: This corresponds to a discriminatory index (D) value of 0.9936.Some of them displayed a relatively low level of diversity in agreement with a restricted ecological niche.These findings provide arguments suggesting that specific S. aureus lineages particularly adapted to ruminant mammary glands have emerged and that MLVA is a convenient tool to provide a broad overview of the population, owing to the availability via internet of databases compiling published MLVA genotypes.

View Article: PubMed Central - PubMed

Affiliation: INRA, UMR1225, IHAP, 31076, Toulouse, France. d.bergonier@envt.fr.

ABSTRACT
Staphylococcus aureus is one of the main etiological agents of mastitis in ruminants. In the present retrospective study, we evaluated the potential interest of a previously described automated multiple loci Variable Number of Tandem Repeats (VNTR) Assay (MLVA) comprising 16 loci as a first line tool to investigate the population structure of S. aureus from mastitis. We determined the genetic diversity of S. aureus strains from cases of clinical and subclinical mastitis in dairy cattle (n = 118, of which 16 were methicillin-resistant), sheep (n = 18) and goats (n = 16). The 152 strains could be subdivided into 115 MLVA genotypes (including 14 genotypes for the ovine strains and 15 genotypes for the caprine strains). This corresponds to a discriminatory index (D) value of 0.9936. Comparison with published MLVA data obtained using the same protocol applied to strains from diverse human and animal origins revealed a low number (8.5%) of human-related MLVA genotypes among the present collection. Eighteen percent of the S. aureus mastitis collection belonged to clonal complexes apparently not associated with other pathological conditions. Some of them displayed a relatively low level of diversity in agreement with a restricted ecological niche. These findings provide arguments suggesting that specific S. aureus lineages particularly adapted to ruminant mammary glands have emerged and that MLVA is a convenient tool to provide a broad overview of the population, owing to the availability via internet of databases compiling published MLVA genotypes.

No MeSH data available.


Related in: MedlinePlus