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In vivo confocal microscopic evaluation of corneal langerhans cells in dry eye patients.

Machetta F, Fea AM, Actis AG, de Sanctis U, Dalmasso P, Grignolo FM - Open Ophthalmol J (2014)

Bottom Line: An increased number of LCs from the center to the periphery of the cornea was observed, significant only in healthy volunteers group.In SSDE group, mean density of LCs in central cornea results also superior to NSSDE group (29,33 cells/mm(2)).This study demonstrates the activation of an inflammatory and immunological reaction in cornea of NSSDE and SSDE patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgical Sciences, University of Torino, Italy.

ABSTRACT

Purpose: To assess inflammatory involvement of cornea in dry eye by means of confocal microscopy, evaluating the presence and distribution of Langherans cells (LCs).

Methods: 98 eyes of 49 subjects were enrolled: 18 subjects affected by Sjögren Syndrome Dry Eye (SSDE), 17 with Non-Sjögren Syndrome Dry Eye (NSSDE), 14 healthy volunteeers. Dry eye symptoms, tear film, ocular surface damage and corneal confocal microscopy were analized.

Results: A significant increase of LCs density was observed at sub-basal nerve plexus (SSDE = 79 cells/mm(2) andNDE = 22 cells/mm(2); p = 0,0031) and sub-epithelial nerve plexus (SSDE = 38 cells/mm(2) and NDE = 3 cells/mm(2); p = 0,0169) in central cornea of SSDE group. An increased number of LCs from the center to the periphery of the cornea was observed, significant only in healthy volunteers group. In dry eye patients there was an increase in LCs density in both peripheral and central cornea with a significant difference between NDE (14,66 cells/mm(2)) and SSDE (56,66 cells/mm(2)) only in central cornea (p = 0,0028). In SSDE group, mean density of LCs in central cornea results also superior to NSSDE group (29,33 cells/mm(2)). There was no correlation between LCs density and dry eye symptoms, tear film deficiency and ocular surface damage.

Conclusion: This study demonstrates the activation of an inflammatory and immunological reaction in cornea of NSSDE and SSDE patients. Confocal microscopy can be an important diagnostic tool in evaluation and follow-up of dry eye disease.

No MeSH data available.


Related in: MedlinePlus

LCs gradient difference between central and peripheral cornea in NDE, NSSDE e SSDE patients.
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Figure 3: LCs gradient difference between central and peripheral cornea in NDE, NSSDE e SSDE patients.

Mentions: LCs were observed especially in the sub-basal nerve plexus in all groups (Table 2). Furthermore the density values of LCs seems to decrease from peripheral corneal to central corneal (Table 2). This gradient difference seems to reduce (even if without statistical significant p = 0.5) in NSSDE patients and particularly in SSDE patients, where the gradient is minimal (NDE: 14.66 cell/mm2versus 44 cell/mm2; NSSDE: 29.33 cell/mm2versus 58.66 cell/mm2; SSDE: 56.66 cell/mm2versus 58.83 cell/mm2) (Fig. 3).


In vivo confocal microscopic evaluation of corneal langerhans cells in dry eye patients.

Machetta F, Fea AM, Actis AG, de Sanctis U, Dalmasso P, Grignolo FM - Open Ophthalmol J (2014)

LCs gradient difference between central and peripheral cornea in NDE, NSSDE e SSDE patients.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4195179&req=5

Figure 3: LCs gradient difference between central and peripheral cornea in NDE, NSSDE e SSDE patients.
Mentions: LCs were observed especially in the sub-basal nerve plexus in all groups (Table 2). Furthermore the density values of LCs seems to decrease from peripheral corneal to central corneal (Table 2). This gradient difference seems to reduce (even if without statistical significant p = 0.5) in NSSDE patients and particularly in SSDE patients, where the gradient is minimal (NDE: 14.66 cell/mm2versus 44 cell/mm2; NSSDE: 29.33 cell/mm2versus 58.66 cell/mm2; SSDE: 56.66 cell/mm2versus 58.83 cell/mm2) (Fig. 3).

Bottom Line: An increased number of LCs from the center to the periphery of the cornea was observed, significant only in healthy volunteers group.In SSDE group, mean density of LCs in central cornea results also superior to NSSDE group (29,33 cells/mm(2)).This study demonstrates the activation of an inflammatory and immunological reaction in cornea of NSSDE and SSDE patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgical Sciences, University of Torino, Italy.

ABSTRACT

Purpose: To assess inflammatory involvement of cornea in dry eye by means of confocal microscopy, evaluating the presence and distribution of Langherans cells (LCs).

Methods: 98 eyes of 49 subjects were enrolled: 18 subjects affected by Sjögren Syndrome Dry Eye (SSDE), 17 with Non-Sjögren Syndrome Dry Eye (NSSDE), 14 healthy volunteeers. Dry eye symptoms, tear film, ocular surface damage and corneal confocal microscopy were analized.

Results: A significant increase of LCs density was observed at sub-basal nerve plexus (SSDE = 79 cells/mm(2) andNDE = 22 cells/mm(2); p = 0,0031) and sub-epithelial nerve plexus (SSDE = 38 cells/mm(2) and NDE = 3 cells/mm(2); p = 0,0169) in central cornea of SSDE group. An increased number of LCs from the center to the periphery of the cornea was observed, significant only in healthy volunteers group. In dry eye patients there was an increase in LCs density in both peripheral and central cornea with a significant difference between NDE (14,66 cells/mm(2)) and SSDE (56,66 cells/mm(2)) only in central cornea (p = 0,0028). In SSDE group, mean density of LCs in central cornea results also superior to NSSDE group (29,33 cells/mm(2)). There was no correlation between LCs density and dry eye symptoms, tear film deficiency and ocular surface damage.

Conclusion: This study demonstrates the activation of an inflammatory and immunological reaction in cornea of NSSDE and SSDE patients. Confocal microscopy can be an important diagnostic tool in evaluation and follow-up of dry eye disease.

No MeSH data available.


Related in: MedlinePlus