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Langerhans cells in lichen planus and lichenoid mucositis an immunohistochemical study.

Devi M, Saraswathi TR, Ranganathan K, Vijayalakshmi D, Sreeja C, Fathima SS - J Pharm Bioallied Sci (2014)

Bottom Line: The mean CD1a LI for LP was significantly higher than that of LM and NM in the basal and supra basal layer.The mean CD1a positive cells in the connective tissues for LP were higher than that of LM and NM.This study clearly demonstrates a statistically significant increase in number of LC in LP than in LM, indicating the possible different immunopathogenic mechanisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Pathology, Adiparasakthi Dental College, Melmaruvathur, Chennai, Tamil Nadu, India.

ABSTRACT

Aim: The aim of this study is to identify and evaluate Langerhans cell (LC) in lichen planus (LP), lichenoid mucositis (LM) and normal mucosa (NM) using CD1a monoclonal antibody immunohistochemically.

Materials and methods: A total of 15 cases of oral lichen planus and 15 cases of LM were selected based on clinical examination and confirmed by histopathological analysis. The biopsies from the 10 patients were taken from normal buccal mucosa as control. Paraffin blocks of tissue were made, which are used for routine hematoxylin and eosin staining and immunohistochemical staining using biotin streptavidin methods (CD1a monoclonal antibody). Analysis of CD1a expression was performed by evaluating the labeling index (LI) for each slide.

Results: The mean CD1a LI for LP was significantly higher than that of LM and NM in the basal and supra basal layer. The mean CD1a positive cells in the connective tissues for LP were higher than that of LM and NM.

Conclusion: This study clearly demonstrates a statistically significant increase in number of LC in LP than in LM, indicating the possible different immunopathogenic mechanisms.

No MeSH data available.


Related in: MedlinePlus

CD1a antibody mean LI in basal and supra basal epithelial layer
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Figure 3: CD1a antibody mean LI in basal and supra basal epithelial layer

Mentions: Data entry and analysis were performed using SPSS version 10.0.5® (SPSS Statistics is a software package used for statistical analysis. Long produced by SPSS Inc., it was acquired by IBM in 2009. SPSS 10.0.5 was released in December 1999). Mean LI and standard deviation were calculated to assess CD1a expression. Analysis of variance was done to compare the mean LI of CD1a between LP, LM and normal mucosa (NM). Bonferroni test of multiple comparison test was done to compare the LI of CD1a between LP, LM, and NM. The difference in mean LI between the study groups for basal layer, suprabasal layer was statistically significant (P = 0.00). The difference in mean positive cells between the study groups for connective tissue layer was statistically significant (P = 0.00) [Table 1 and Figures 3 and 4]. The mean difference in Mean LI in basal layer of LP and LM was 2.96 and it was statistically significant (P = 0.001); in LP and NM was 5.54 and it was statistically significant (P = 0.00); in LM and NM was 1.49 and it was statistically not significant (P = 0.21).


Langerhans cells in lichen planus and lichenoid mucositis an immunohistochemical study.

Devi M, Saraswathi TR, Ranganathan K, Vijayalakshmi D, Sreeja C, Fathima SS - J Pharm Bioallied Sci (2014)

CD1a antibody mean LI in basal and supra basal epithelial layer
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4157254&req=5

Figure 3: CD1a antibody mean LI in basal and supra basal epithelial layer
Mentions: Data entry and analysis were performed using SPSS version 10.0.5® (SPSS Statistics is a software package used for statistical analysis. Long produced by SPSS Inc., it was acquired by IBM in 2009. SPSS 10.0.5 was released in December 1999). Mean LI and standard deviation were calculated to assess CD1a expression. Analysis of variance was done to compare the mean LI of CD1a between LP, LM and normal mucosa (NM). Bonferroni test of multiple comparison test was done to compare the LI of CD1a between LP, LM, and NM. The difference in mean LI between the study groups for basal layer, suprabasal layer was statistically significant (P = 0.00). The difference in mean positive cells between the study groups for connective tissue layer was statistically significant (P = 0.00) [Table 1 and Figures 3 and 4]. The mean difference in Mean LI in basal layer of LP and LM was 2.96 and it was statistically significant (P = 0.001); in LP and NM was 5.54 and it was statistically significant (P = 0.00); in LM and NM was 1.49 and it was statistically not significant (P = 0.21).

Bottom Line: The mean CD1a LI for LP was significantly higher than that of LM and NM in the basal and supra basal layer.The mean CD1a positive cells in the connective tissues for LP were higher than that of LM and NM.This study clearly demonstrates a statistically significant increase in number of LC in LP than in LM, indicating the possible different immunopathogenic mechanisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Pathology, Adiparasakthi Dental College, Melmaruvathur, Chennai, Tamil Nadu, India.

ABSTRACT

Aim: The aim of this study is to identify and evaluate Langerhans cell (LC) in lichen planus (LP), lichenoid mucositis (LM) and normal mucosa (NM) using CD1a monoclonal antibody immunohistochemically.

Materials and methods: A total of 15 cases of oral lichen planus and 15 cases of LM were selected based on clinical examination and confirmed by histopathological analysis. The biopsies from the 10 patients were taken from normal buccal mucosa as control. Paraffin blocks of tissue were made, which are used for routine hematoxylin and eosin staining and immunohistochemical staining using biotin streptavidin methods (CD1a monoclonal antibody). Analysis of CD1a expression was performed by evaluating the labeling index (LI) for each slide.

Results: The mean CD1a LI for LP was significantly higher than that of LM and NM in the basal and supra basal layer. The mean CD1a positive cells in the connective tissues for LP were higher than that of LM and NM.

Conclusion: This study clearly demonstrates a statistically significant increase in number of LC in LP than in LM, indicating the possible different immunopathogenic mechanisms.

No MeSH data available.


Related in: MedlinePlus