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Effects of deuterium oxide on cell growth and vesicle speed in RBL-2H3 cells.

Kalkur RS, Ballast AC, Triplett AR, Spendier K - PeerJ (2014)

Bottom Line: For the first time we show the effects of deuterium oxide on cell growth and vesicle transport in rat basophilic leukemia (RBL-2H3) cells.RBL-2H3 cells cultured with 15 moles/L deuterium showed decreased cell growth which was attributed to cells not doubling their DNA content.This increase in vesicle speed was not observed in deuterium oxide cultures treated with a microtubule-destabilizing drug, suggesting that deuterium oxide affects microtubule-dependent vesicle transport.

View Article: PubMed Central - HTML - PubMed

Affiliation: BioFrontiers Center, University of Colorado at Colorado Springs , Colorado Springs, CO , USA.

ABSTRACT
For the first time we show the effects of deuterium oxide on cell growth and vesicle transport in rat basophilic leukemia (RBL-2H3) cells. RBL-2H3 cells cultured with 15 moles/L deuterium showed decreased cell growth which was attributed to cells not doubling their DNA content. Experimental observations also showed an increase in vesicle speed for cells cultured in deuterium oxide. This increase in vesicle speed was not observed in deuterium oxide cultures treated with a microtubule-destabilizing drug, suggesting that deuterium oxide affects microtubule-dependent vesicle transport.

No MeSH data available.


Related in: MedlinePlus

The effect of deuterium oxide on RBL-2H3 cell cycle as shown by propidium iodide intensity (FL3) histogram plots.Cell counts were normalized to unit area. Cells grown (A) in media containing 0 moles/L D2O and (B) in media containing 15 moles/L D2O. The black lines represent one-day old cultures and the red lines represent four-day old cultures. The arrows indicates position of the G2–M-cell cycle phase.
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fig-2: The effect of deuterium oxide on RBL-2H3 cell cycle as shown by propidium iodide intensity (FL3) histogram plots.Cell counts were normalized to unit area. Cells grown (A) in media containing 0 moles/L D2O and (B) in media containing 15 moles/L D2O. The black lines represent one-day old cultures and the red lines represent four-day old cultures. The arrows indicates position of the G2–M-cell cycle phase.

Mentions: Figure 2 depicts DNA content frequency histograms for RBL-2H3 cells in untreated cultures (Fig. 2A) and in cultures treated with D2O (Fig. 2B). The presented data was smoothed using a moving average and the area underneath the curve was normalized to unity. For 0 moles/L D2O treated cells, the G2–M-phase (black arrow) is recognizable in one-day (black line in Fig. 2A) and four-day (red line in Fig. 2A) old cultures. In other 0 moles/L D2O cell cultures (data not shown) the G2–M peak was more pronounced. In one-day old D2O treated cultures, the G2–M-phase can also be identified (black line in Fig. 2B). The number of cells entering the G2–M-phase in four-day old D2O cultures decreases, as shown by a weakened G2–M-phase and an increased height of the G0–G1 peak (red line in Fig. 2B). The loss of the G2–M peak reflects that RBL-2H3 cells have not doubled their DNA content. This loss of DNA content may be caused by cells failing to enter mitosis and/or is a sign that cells are in the early stages of apoptosis. Both mechanisms were reported previously in human pancreatic tumor cells in which D2O induced apoptosis in PANC-1 and AsPC-1 cells and arrested PANC-1 and BxPC-3 cells in the G2–M-phase of the cell cycle (Hartmann et al., 2005). We also note that the G0–G1 peaks for both D2O treated and untreated cultures experience a shift of approximately the same magnitude to the left within four days. Such shifts can be attributed to daily fluctuations in the flow cytometry excitation and detection system.


Effects of deuterium oxide on cell growth and vesicle speed in RBL-2H3 cells.

Kalkur RS, Ballast AC, Triplett AR, Spendier K - PeerJ (2014)

The effect of deuterium oxide on RBL-2H3 cell cycle as shown by propidium iodide intensity (FL3) histogram plots.Cell counts were normalized to unit area. Cells grown (A) in media containing 0 moles/L D2O and (B) in media containing 15 moles/L D2O. The black lines represent one-day old cultures and the red lines represent four-day old cultures. The arrows indicates position of the G2–M-cell cycle phase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4157235&req=5

fig-2: The effect of deuterium oxide on RBL-2H3 cell cycle as shown by propidium iodide intensity (FL3) histogram plots.Cell counts were normalized to unit area. Cells grown (A) in media containing 0 moles/L D2O and (B) in media containing 15 moles/L D2O. The black lines represent one-day old cultures and the red lines represent four-day old cultures. The arrows indicates position of the G2–M-cell cycle phase.
Mentions: Figure 2 depicts DNA content frequency histograms for RBL-2H3 cells in untreated cultures (Fig. 2A) and in cultures treated with D2O (Fig. 2B). The presented data was smoothed using a moving average and the area underneath the curve was normalized to unity. For 0 moles/L D2O treated cells, the G2–M-phase (black arrow) is recognizable in one-day (black line in Fig. 2A) and four-day (red line in Fig. 2A) old cultures. In other 0 moles/L D2O cell cultures (data not shown) the G2–M peak was more pronounced. In one-day old D2O treated cultures, the G2–M-phase can also be identified (black line in Fig. 2B). The number of cells entering the G2–M-phase in four-day old D2O cultures decreases, as shown by a weakened G2–M-phase and an increased height of the G0–G1 peak (red line in Fig. 2B). The loss of the G2–M peak reflects that RBL-2H3 cells have not doubled their DNA content. This loss of DNA content may be caused by cells failing to enter mitosis and/or is a sign that cells are in the early stages of apoptosis. Both mechanisms were reported previously in human pancreatic tumor cells in which D2O induced apoptosis in PANC-1 and AsPC-1 cells and arrested PANC-1 and BxPC-3 cells in the G2–M-phase of the cell cycle (Hartmann et al., 2005). We also note that the G0–G1 peaks for both D2O treated and untreated cultures experience a shift of approximately the same magnitude to the left within four days. Such shifts can be attributed to daily fluctuations in the flow cytometry excitation and detection system.

Bottom Line: For the first time we show the effects of deuterium oxide on cell growth and vesicle transport in rat basophilic leukemia (RBL-2H3) cells.RBL-2H3 cells cultured with 15 moles/L deuterium showed decreased cell growth which was attributed to cells not doubling their DNA content.This increase in vesicle speed was not observed in deuterium oxide cultures treated with a microtubule-destabilizing drug, suggesting that deuterium oxide affects microtubule-dependent vesicle transport.

View Article: PubMed Central - HTML - PubMed

Affiliation: BioFrontiers Center, University of Colorado at Colorado Springs , Colorado Springs, CO , USA.

ABSTRACT
For the first time we show the effects of deuterium oxide on cell growth and vesicle transport in rat basophilic leukemia (RBL-2H3) cells. RBL-2H3 cells cultured with 15 moles/L deuterium showed decreased cell growth which was attributed to cells not doubling their DNA content. Experimental observations also showed an increase in vesicle speed for cells cultured in deuterium oxide. This increase in vesicle speed was not observed in deuterium oxide cultures treated with a microtubule-destabilizing drug, suggesting that deuterium oxide affects microtubule-dependent vesicle transport.

No MeSH data available.


Related in: MedlinePlus