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Remineralization and repair of enamel surface by biomimetic Zn-carbonate hydroxyapatite containing toothpaste: a comparative in vivo study.

Lelli M, Putignano A, Marchetti M, Foltran I, Mangani F, Procaccini M, Roveri N, Orsini G - Front Physiol (2014)

Bottom Line: Teeth were processed for morphological and chemical-physic superficial characterizations by means of Scanning Electronic Microscopy with Elementary analysis, X-Ray Diffraction analysis and Infrared analysis.On the other hand, the use of both a nitrate potassium/sodium fluoride and non-specified fluoride toothpastes did not appreciably change the enamel surface.In conclusion, this study demonstrates that the toothpaste containing Zn-CHA nanostructured microcrystals, differently from nitrate potassium/sodium fluoride and non-specified fluoride toothpastes, may promote enamel superficial repair by means of the formation of a protective biomimetic CHA coating.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry "G. Ciamician," Alma Mater Studiorum, University of Bologna Bologna, Italy.

ABSTRACT
Consumption of acidic foods and drinks and other factors that cause enamel wear are responsible for the daily enamel loss and degradation. Use of some toothpastes that have been showed to possess different properties of remineralisation and/or repair of the enamel surface may help to protect tooth enamel. The aim of this study was to evaluate whether the use of toothpaste containing Zn-carbonate hydroxyapatite (CHA) nanostructured microcrystals may exert remineralization/repair effects of the enamel surface. Two groups of patients, aged between 18 and 75 years, used a Zn-CHA nanocrystals-based toothpaste (experimental group) and a potassium nitrate/sodium fluoride toothpaste (active control group) for 8 weeks. At the end of this period, extractions were performed in five subjects per study group. Negative controls consisted of two subjects treated with non-specified fluoride toothpaste. Teeth were processed for morphological and chemical-physic superficial characterizations by means of Scanning Electronic Microscopy with Elementary analysis, X-Ray Diffraction analysis and Infrared analysis. In this study, the use of a Zn-CHA nanocrystals toothpaste led to a remineralization/repair of the enamel surface, by deposition of a hydroxyapatite-rich coating. On the other hand, the use of both a nitrate potassium/sodium fluoride and non-specified fluoride toothpastes did not appreciably change the enamel surface. In conclusion, this study demonstrates that the toothpaste containing Zn-CHA nanostructured microcrystals, differently from nitrate potassium/sodium fluoride and non-specified fluoride toothpastes, may promote enamel superficial repair by means of the formation of a protective biomimetic CHA coating.

No MeSH data available.


Related in: MedlinePlus

X-ray diffraction patterns collected for synthetic biomimetic CHA nanocrystals (A), CHA nanostructured microcrystals (B), and human tooth enamel (C).
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Figure 2: X-ray diffraction patterns collected for synthetic biomimetic CHA nanocrystals (A), CHA nanostructured microcrystals (B), and human tooth enamel (C).

Mentions: The XRD of synthetic biomimetic CHA nanocrystals, showing the broadened diffraction maxima of an apatite single phase, is reported in Figure 2A. This pattern is compared with the XRD collected from the synthetic biomimetic CHA nanostructured microclusters used for preparing the Zn-CHA toothpaste (Figure 2B) and the XRD of human tooth enamel (Figure 2C). There are evident similarities between Figures 2A,B, whereas Figure 2C shows the highest degree of crystallinity, proper of the natural enamel. The FT-IR spectra of synthesized CHA nanostructured microclusters and human enamel apatite are reported in Figures 3A,B, respectively. In both these spectra, it is possible to find characteristic bands at 1032 - 1104 cm−1, due to the phosphate group. The absorption band at 1472 cm−1 is related to the carbonate group substitution of the phosphate one, while the shoulder at 1550 cm−1 can be attributed to the carbonate group substituting the hydroxyl group in the apatite structure.


Remineralization and repair of enamel surface by biomimetic Zn-carbonate hydroxyapatite containing toothpaste: a comparative in vivo study.

Lelli M, Putignano A, Marchetti M, Foltran I, Mangani F, Procaccini M, Roveri N, Orsini G - Front Physiol (2014)

X-ray diffraction patterns collected for synthetic biomimetic CHA nanocrystals (A), CHA nanostructured microcrystals (B), and human tooth enamel (C).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4155874&req=5

Figure 2: X-ray diffraction patterns collected for synthetic biomimetic CHA nanocrystals (A), CHA nanostructured microcrystals (B), and human tooth enamel (C).
Mentions: The XRD of synthetic biomimetic CHA nanocrystals, showing the broadened diffraction maxima of an apatite single phase, is reported in Figure 2A. This pattern is compared with the XRD collected from the synthetic biomimetic CHA nanostructured microclusters used for preparing the Zn-CHA toothpaste (Figure 2B) and the XRD of human tooth enamel (Figure 2C). There are evident similarities between Figures 2A,B, whereas Figure 2C shows the highest degree of crystallinity, proper of the natural enamel. The FT-IR spectra of synthesized CHA nanostructured microclusters and human enamel apatite are reported in Figures 3A,B, respectively. In both these spectra, it is possible to find characteristic bands at 1032 - 1104 cm−1, due to the phosphate group. The absorption band at 1472 cm−1 is related to the carbonate group substitution of the phosphate one, while the shoulder at 1550 cm−1 can be attributed to the carbonate group substituting the hydroxyl group in the apatite structure.

Bottom Line: Teeth were processed for morphological and chemical-physic superficial characterizations by means of Scanning Electronic Microscopy with Elementary analysis, X-Ray Diffraction analysis and Infrared analysis.On the other hand, the use of both a nitrate potassium/sodium fluoride and non-specified fluoride toothpastes did not appreciably change the enamel surface.In conclusion, this study demonstrates that the toothpaste containing Zn-CHA nanostructured microcrystals, differently from nitrate potassium/sodium fluoride and non-specified fluoride toothpastes, may promote enamel superficial repair by means of the formation of a protective biomimetic CHA coating.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry "G. Ciamician," Alma Mater Studiorum, University of Bologna Bologna, Italy.

ABSTRACT
Consumption of acidic foods and drinks and other factors that cause enamel wear are responsible for the daily enamel loss and degradation. Use of some toothpastes that have been showed to possess different properties of remineralisation and/or repair of the enamel surface may help to protect tooth enamel. The aim of this study was to evaluate whether the use of toothpaste containing Zn-carbonate hydroxyapatite (CHA) nanostructured microcrystals may exert remineralization/repair effects of the enamel surface. Two groups of patients, aged between 18 and 75 years, used a Zn-CHA nanocrystals-based toothpaste (experimental group) and a potassium nitrate/sodium fluoride toothpaste (active control group) for 8 weeks. At the end of this period, extractions were performed in five subjects per study group. Negative controls consisted of two subjects treated with non-specified fluoride toothpaste. Teeth were processed for morphological and chemical-physic superficial characterizations by means of Scanning Electronic Microscopy with Elementary analysis, X-Ray Diffraction analysis and Infrared analysis. In this study, the use of a Zn-CHA nanocrystals toothpaste led to a remineralization/repair of the enamel surface, by deposition of a hydroxyapatite-rich coating. On the other hand, the use of both a nitrate potassium/sodium fluoride and non-specified fluoride toothpastes did not appreciably change the enamel surface. In conclusion, this study demonstrates that the toothpaste containing Zn-CHA nanostructured microcrystals, differently from nitrate potassium/sodium fluoride and non-specified fluoride toothpastes, may promote enamel superficial repair by means of the formation of a protective biomimetic CHA coating.

No MeSH data available.


Related in: MedlinePlus