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In vivo assessment of antiretroviral therapy-associated side effects.

Ramos-Sanchez EM, Goto H, Rivero DH, Mauad T, de Souza FN, Monteiro AM, Gidlund M - Mem. Inst. Oswaldo Cruz (2014)

Bottom Line: Antiretroviral therapy has been associated with side effects, either from the drug itself or in conjunction with the effects of human immunodeficiency virus infection.Indinavir treatment increased the hamster death rate and resulted in an increase in triglyceride, cholesterol and glucose serum levels and a reduction in anti-oxLDL auto-antibodies.The treatment led to histopathological alterations of the kidney and the heart.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Imunologia, Instituto de Ciências Biomédicas, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.

ABSTRACT
Antiretroviral therapy has been associated with side effects, either from the drug itself or in conjunction with the effects of human immunodeficiency virus infection. Here, we evaluated the side effects of the protease inhibitor (PI) indinavir in hamsters consuming a normal or high-fat diet. Indinavir treatment increased the hamster death rate and resulted in an increase in triglyceride, cholesterol and glucose serum levels and a reduction in anti-oxLDL auto-antibodies. The treatment led to histopathological alterations of the kidney and the heart. These results suggest that hamsters are an interesting model for the study of the side effects of antiretroviral drugs, such as PIs.

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Related in: MedlinePlus

Histological evaluation of heart and kidney tissues of Mesocricetusauratus under indinavir treatment with or without high fat-diet.Hamster heart (A-D) and kidney (E-H) histological sections stained with Masson’strichrome dye (40X). Areas of fibrosis stained in blue are indicated by arrows. A,E: normal diet without treatment; B, F: normal diet with 30 mg/kg/day indinavirtreatment; C, G: high-fat diet without treatment; D, H: high-fat diet with 30mg/kg/day indinavir treatment.
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f01: Histological evaluation of heart and kidney tissues of Mesocricetusauratus under indinavir treatment with or without high fat-diet.Hamster heart (A-D) and kidney (E-H) histological sections stained with Masson’strichrome dye (40X). Areas of fibrosis stained in blue are indicated by arrows. A,E: normal diet without treatment; B, F: normal diet with 30 mg/kg/day indinavirtreatment; C, G: high-fat diet without treatment; D, H: high-fat diet with 30mg/kg/day indinavir treatment.

Mentions: The histological evaluation of heart tissues stained with Masson’s trichrome dye showedareas of fibrosis in animals treated with indinavir under both the normal and the high-fatdiet conditions (B, D in Figure) compared withuntreated control animals (A, C in Figure). Nolesions suggestive of atherosclerosis were found in either the aortas or the carotidarteries in the samples stained with H&E or Oil Red O.


In vivo assessment of antiretroviral therapy-associated side effects.

Ramos-Sanchez EM, Goto H, Rivero DH, Mauad T, de Souza FN, Monteiro AM, Gidlund M - Mem. Inst. Oswaldo Cruz (2014)

Histological evaluation of heart and kidney tissues of Mesocricetusauratus under indinavir treatment with or without high fat-diet.Hamster heart (A-D) and kidney (E-H) histological sections stained with Masson’strichrome dye (40X). Areas of fibrosis stained in blue are indicated by arrows. A,E: normal diet without treatment; B, F: normal diet with 30 mg/kg/day indinavirtreatment; C, G: high-fat diet without treatment; D, H: high-fat diet with 30mg/kg/day indinavir treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4155852&req=5

f01: Histological evaluation of heart and kidney tissues of Mesocricetusauratus under indinavir treatment with or without high fat-diet.Hamster heart (A-D) and kidney (E-H) histological sections stained with Masson’strichrome dye (40X). Areas of fibrosis stained in blue are indicated by arrows. A,E: normal diet without treatment; B, F: normal diet with 30 mg/kg/day indinavirtreatment; C, G: high-fat diet without treatment; D, H: high-fat diet with 30mg/kg/day indinavir treatment.
Mentions: The histological evaluation of heart tissues stained with Masson’s trichrome dye showedareas of fibrosis in animals treated with indinavir under both the normal and the high-fatdiet conditions (B, D in Figure) compared withuntreated control animals (A, C in Figure). Nolesions suggestive of atherosclerosis were found in either the aortas or the carotidarteries in the samples stained with H&E or Oil Red O.

Bottom Line: Antiretroviral therapy has been associated with side effects, either from the drug itself or in conjunction with the effects of human immunodeficiency virus infection.Indinavir treatment increased the hamster death rate and resulted in an increase in triglyceride, cholesterol and glucose serum levels and a reduction in anti-oxLDL auto-antibodies.The treatment led to histopathological alterations of the kidney and the heart.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Imunologia, Instituto de Ciências Biomédicas, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.

ABSTRACT
Antiretroviral therapy has been associated with side effects, either from the drug itself or in conjunction with the effects of human immunodeficiency virus infection. Here, we evaluated the side effects of the protease inhibitor (PI) indinavir in hamsters consuming a normal or high-fat diet. Indinavir treatment increased the hamster death rate and resulted in an increase in triglyceride, cholesterol and glucose serum levels and a reduction in anti-oxLDL auto-antibodies. The treatment led to histopathological alterations of the kidney and the heart. These results suggest that hamsters are an interesting model for the study of the side effects of antiretroviral drugs, such as PIs.

Show MeSH
Related in: MedlinePlus