Limits...
Limits of a rapid identification of common Mediterranean sandflies using polymerase chain reaction-restriction fragment length polymorphism.

Bounamous A, Lehrter V, Hadj-Henni L, Delecolle JC, Depaquit J - Mem. Inst. Oswaldo Cruz (2014)

Bottom Line: Our study highlights two sympatric populations within P. sergenti in the area of its type-locality and new haplotypes of P. perniciosus and P. longicuspis without recording the specimens called lcx previously found in North Africa.We tried to use a polymerase chain reaction-restriction fragment length polymorphism method based on a combined double digestion of each marker.These method is not interesting to identify sandflies all over the Mediterranean Basin.

View Article: PubMed Central - PubMed

Affiliation: Agence Nationale de la Sécurité Sanitaire de l?Alimentation, de l?Enviromment et du Travail, Faculté de Pharmacie, Université de Reims Champagne-Ardenne, Reims, France.

ABSTRACT
A total of 131 phlebotomine Algerian sandflies have been processed in the present study. They belong to the species Phlebotomus bergeroti, Phlebotomus alexandri, Phlebotomus sergenti, Phlebotomus chabaudi, Phlebotomus riouxi, Phlebotomus perniciosus, Phlebotomus longicuspis, Phlebotomus perfiliewi, Phlebotomus ariasi, Phlebotomus chadlii, Sergentomyia fallax, Sergentomyia minuta, Sergentomyia antennata, Sergentomyia schwetzi, Sergentomyia clydei, Sergentomyia christophersi and Grassomyia dreyfussi. They have been characterised by sequencing of a part of the cytochrome b (cyt b), t RNA serine and NADH1 on the one hand and of the cytochrome C oxidase I of the mitochondrial DNA (mtDNA) on the other hand. Our study highlights two sympatric populations within P. sergenti in the area of its type-locality and new haplotypes of P. perniciosus and P. longicuspis without recording the specimens called lcx previously found in North Africa. We tried to use a polymerase chain reaction-restriction fragment length polymorphism method based on a combined double digestion of each marker. These method is not interesting to identify sandflies all over the Mediterranean Basin.

Show MeSH
: neighbour-joining tree based on mitochondrial DNA cytochrome b sequencesrooted on Phlebotomus bergeroti. Bootstrap values after 1,000replicates are indicated on the branches.
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f02: : neighbour-joining tree based on mitochondrial DNA cytochrome b sequencesrooted on Phlebotomus bergeroti. Bootstrap values after 1,000replicates are indicated on the branches.

Mentions: Global trees based on cyt b and COI sequences arepresented in Figs 2, 3, respectively. According to Depaquit et al. (1998b), Phlebotomus bergeroti has beenselected to root the tree. All the species morphologically recognised are wellindividualised. We note that P. sergenti included two populationswithout any morphological difference. The identification of P.perniciosus and P. longicuspis is not doubtful. We did notrecord any atypical specimen.


Limits of a rapid identification of common Mediterranean sandflies using polymerase chain reaction-restriction fragment length polymorphism.

Bounamous A, Lehrter V, Hadj-Henni L, Delecolle JC, Depaquit J - Mem. Inst. Oswaldo Cruz (2014)

: neighbour-joining tree based on mitochondrial DNA cytochrome b sequencesrooted on Phlebotomus bergeroti. Bootstrap values after 1,000replicates are indicated on the branches.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4155849&req=5

f02: : neighbour-joining tree based on mitochondrial DNA cytochrome b sequencesrooted on Phlebotomus bergeroti. Bootstrap values after 1,000replicates are indicated on the branches.
Mentions: Global trees based on cyt b and COI sequences arepresented in Figs 2, 3, respectively. According to Depaquit et al. (1998b), Phlebotomus bergeroti has beenselected to root the tree. All the species morphologically recognised are wellindividualised. We note that P. sergenti included two populationswithout any morphological difference. The identification of P.perniciosus and P. longicuspis is not doubtful. We did notrecord any atypical specimen.

Bottom Line: Our study highlights two sympatric populations within P. sergenti in the area of its type-locality and new haplotypes of P. perniciosus and P. longicuspis without recording the specimens called lcx previously found in North Africa.We tried to use a polymerase chain reaction-restriction fragment length polymorphism method based on a combined double digestion of each marker.These method is not interesting to identify sandflies all over the Mediterranean Basin.

View Article: PubMed Central - PubMed

Affiliation: Agence Nationale de la Sécurité Sanitaire de l?Alimentation, de l?Enviromment et du Travail, Faculté de Pharmacie, Université de Reims Champagne-Ardenne, Reims, France.

ABSTRACT
A total of 131 phlebotomine Algerian sandflies have been processed in the present study. They belong to the species Phlebotomus bergeroti, Phlebotomus alexandri, Phlebotomus sergenti, Phlebotomus chabaudi, Phlebotomus riouxi, Phlebotomus perniciosus, Phlebotomus longicuspis, Phlebotomus perfiliewi, Phlebotomus ariasi, Phlebotomus chadlii, Sergentomyia fallax, Sergentomyia minuta, Sergentomyia antennata, Sergentomyia schwetzi, Sergentomyia clydei, Sergentomyia christophersi and Grassomyia dreyfussi. They have been characterised by sequencing of a part of the cytochrome b (cyt b), t RNA serine and NADH1 on the one hand and of the cytochrome C oxidase I of the mitochondrial DNA (mtDNA) on the other hand. Our study highlights two sympatric populations within P. sergenti in the area of its type-locality and new haplotypes of P. perniciosus and P. longicuspis without recording the specimens called lcx previously found in North Africa. We tried to use a polymerase chain reaction-restriction fragment length polymorphism method based on a combined double digestion of each marker. These method is not interesting to identify sandflies all over the Mediterranean Basin.

Show MeSH