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Molecular epidemiology of Candida albicans and Candida glabrata strains isolated from intensive care unit patients in Poland.

Paluchowska P, Tokarczyk M, Bogusz B, Skiba I, Budak A - Mem. Inst. Oswaldo Cruz (2014)

Bottom Line: The results of the present investigation revealed a high degree of genetic diversity among the isolates.No clonal relationship was found among the C. albicans strains, whereas two C. glabrata isolates were identical.The source of Candida infection appeared to be mostly endogenous; however, the presence of two clonal C. glabrata strains suggested the possibility of cross-transmission of these pathogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland.

ABSTRACT
Over the last decades, Candida spp have been responsible for an increasing number of infections, especially in patients requiring intensive care. Knowledge of local epidemiology and analysis of the spread of these pathogens is important in understanding and controlling their transmission. The aim of this study was to evaluate the genetic diversity of 31 Candida albicans and 17 Candida glabrata isolates recovered from intensive care unit patients from the tertiary hospital in Krakow between 2011-2012. The strains were typed by random amplified polymorphic DNA (RAPD) polymerase chain reaction using five primers (CD16AS, HP1247, ERIC-2, OPE-3 and OPE-18). The results of the present investigation revealed a high degree of genetic diversity among the isolates. No clonal relationship was found among the C. albicans strains, whereas two C. glabrata isolates were identical. The source of Candida infection appeared to be mostly endogenous; however, the presence of two clonal C. glabrata strains suggested the possibility of cross-transmission of these pathogens. Our study confirmed the high discriminatory power of the RAPD technique in the molecular typing of Candida clinical isolates. This method may be applied to the evaluation of transmission routes of pathogenic fungi on a local level.

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Related in: MedlinePlus

: dendrogram presenting the genetic relatedness of 31 Candidaalbicans strains from intensive care unit patients generated byrandom amplified polymorphic DNA polymerase chain reaction, using CD16AS,HP1247, ERIC-2, OPE-3 and OPE-18 primers. The vertical line divides the strainsaccording to the level of genetic similarity into related andunrelated.
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f01: : dendrogram presenting the genetic relatedness of 31 Candidaalbicans strains from intensive care unit patients generated byrandom amplified polymorphic DNA polymerase chain reaction, using CD16AS,HP1247, ERIC-2, OPE-3 and OPE-18 primers. The vertical line divides the strainsaccording to the level of genetic similarity into related andunrelated.

Mentions: Among 31 C. albicans strains, 31 RAPD profiles were distinguished.Twenty C. albicans isolates were allocated into nine clusters (E, H-K,M, O, T, U) with homology levels higher than 80%, whereas the remaining 11 genotypes(A-D, F, G, L, N, P, R, S) were represented by single isolates only. Four isolatesbelonged to cluster E, while clusters H-K, M, O, T and U consisted of two strains each(Fig. 1).


Molecular epidemiology of Candida albicans and Candida glabrata strains isolated from intensive care unit patients in Poland.

Paluchowska P, Tokarczyk M, Bogusz B, Skiba I, Budak A - Mem. Inst. Oswaldo Cruz (2014)

: dendrogram presenting the genetic relatedness of 31 Candidaalbicans strains from intensive care unit patients generated byrandom amplified polymorphic DNA polymerase chain reaction, using CD16AS,HP1247, ERIC-2, OPE-3 and OPE-18 primers. The vertical line divides the strainsaccording to the level of genetic similarity into related andunrelated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4155844&req=5

f01: : dendrogram presenting the genetic relatedness of 31 Candidaalbicans strains from intensive care unit patients generated byrandom amplified polymorphic DNA polymerase chain reaction, using CD16AS,HP1247, ERIC-2, OPE-3 and OPE-18 primers. The vertical line divides the strainsaccording to the level of genetic similarity into related andunrelated.
Mentions: Among 31 C. albicans strains, 31 RAPD profiles were distinguished.Twenty C. albicans isolates were allocated into nine clusters (E, H-K,M, O, T, U) with homology levels higher than 80%, whereas the remaining 11 genotypes(A-D, F, G, L, N, P, R, S) were represented by single isolates only. Four isolatesbelonged to cluster E, while clusters H-K, M, O, T and U consisted of two strains each(Fig. 1).

Bottom Line: The results of the present investigation revealed a high degree of genetic diversity among the isolates.No clonal relationship was found among the C. albicans strains, whereas two C. glabrata isolates were identical.The source of Candida infection appeared to be mostly endogenous; however, the presence of two clonal C. glabrata strains suggested the possibility of cross-transmission of these pathogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland.

ABSTRACT
Over the last decades, Candida spp have been responsible for an increasing number of infections, especially in patients requiring intensive care. Knowledge of local epidemiology and analysis of the spread of these pathogens is important in understanding and controlling their transmission. The aim of this study was to evaluate the genetic diversity of 31 Candida albicans and 17 Candida glabrata isolates recovered from intensive care unit patients from the tertiary hospital in Krakow between 2011-2012. The strains were typed by random amplified polymorphic DNA (RAPD) polymerase chain reaction using five primers (CD16AS, HP1247, ERIC-2, OPE-3 and OPE-18). The results of the present investigation revealed a high degree of genetic diversity among the isolates. No clonal relationship was found among the C. albicans strains, whereas two C. glabrata isolates were identical. The source of Candida infection appeared to be mostly endogenous; however, the presence of two clonal C. glabrata strains suggested the possibility of cross-transmission of these pathogens. Our study confirmed the high discriminatory power of the RAPD technique in the molecular typing of Candida clinical isolates. This method may be applied to the evaluation of transmission routes of pathogenic fungi on a local level.

Show MeSH
Related in: MedlinePlus