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Acute gastroenteritis and enteric viruses in hospitalised children in southern Brazil: aetiology, seasonality and clinical outcomes.

Raboni SM, Damasio GA, Ferreira CE, Pereira LA, Nogueira MB, Vidal LR, Cruz CR, Almeida SM - Mem. Inst. Oswaldo Cruz (2014)

Bottom Line: Mixed infections were found in nine patients: HAdV/RVA (5), HAdV/NoV (3) and HAdV/NoV/RVA (1).The frequency of fever and lymphocytosis was significantly higher in virus-infected patients.The significant frequency of these pathogens in patients with AG highlights the need to routinely implement laboratory investigations.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Virologia, Hospital de Clínicas, Departamento de Pediatria, Universidade Federal do Paraná, Curitiba, PR, Brasil.

ABSTRACT
Viral acute gastroenteritis (AG) is a significant cause of hospitalisation in children younger than five years. Group A rotavirus (RVA) is responsible for 30% of these cases. Following the introduction of RVA immunisation in Brazil in 2006, a decreased circulation of this virus has been observed. However, AG remains an important cause of hospitalisation of paediatric patients and only limited data are available regarding the role of other enteric viruses in these cases. We conducted a prospective study of paediatric patients hospitalised for AG. Stool samples were collected to investigate human adenovirus (HAdV), RVA, norovirus (NoV) and astrovirus (AstV). NoV typing was performed by nucleotide sequencing and phylogenetic analysis. From the 225 samples tested, 60 (26%) were positive for at least one viral agent. HAdV, NoV, RVA and AstV were detected in 16%, 8%, 6% and 0% of the samples, respectively. Mixed infections were found in nine patients: HAdV/RVA (5), HAdV/NoV (3) and HAdV/NoV/RVA (1). The frequency of fever and lymphocytosis was significantly higher in virus-infected patients. Phylogenetic analysis of NoV indicated that all of these viruses belonged to genotype GII.4. The significant frequency of these pathogens in patients with AG highlights the need to routinely implement laboratory investigations.

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: distribution of positive samples for human adenovirus, norovirus, group Arotavirus and the correlation between rainfall and mean temperature fromSeptember 2010-September 2011. Source: Meteorological System of Paraná.
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f01: : distribution of positive samples for human adenovirus, norovirus, group Arotavirus and the correlation between rainfall and mean temperature fromSeptember 2010-September 2011. Source: Meteorological System of Paraná.

Mentions: The mean number of stool samples sent to the laboratory was approximately 17samples/month (± 4.8), with an average of five positive samples/month (± 2.6). Thedistribution of positive samples and its relation to the average monthly temperature(ºC) and precipitation (cm3) between September 2010-September 2011 revealedthe absence of a seasonal pattern for the studied viruses, as the pathogen detectionsoccurred throughout the year (Fig. 1).


Acute gastroenteritis and enteric viruses in hospitalised children in southern Brazil: aetiology, seasonality and clinical outcomes.

Raboni SM, Damasio GA, Ferreira CE, Pereira LA, Nogueira MB, Vidal LR, Cruz CR, Almeida SM - Mem. Inst. Oswaldo Cruz (2014)

: distribution of positive samples for human adenovirus, norovirus, group Arotavirus and the correlation between rainfall and mean temperature fromSeptember 2010-September 2011. Source: Meteorological System of Paraná.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4155843&req=5

f01: : distribution of positive samples for human adenovirus, norovirus, group Arotavirus and the correlation between rainfall and mean temperature fromSeptember 2010-September 2011. Source: Meteorological System of Paraná.
Mentions: The mean number of stool samples sent to the laboratory was approximately 17samples/month (± 4.8), with an average of five positive samples/month (± 2.6). Thedistribution of positive samples and its relation to the average monthly temperature(ºC) and precipitation (cm3) between September 2010-September 2011 revealedthe absence of a seasonal pattern for the studied viruses, as the pathogen detectionsoccurred throughout the year (Fig. 1).

Bottom Line: Mixed infections were found in nine patients: HAdV/RVA (5), HAdV/NoV (3) and HAdV/NoV/RVA (1).The frequency of fever and lymphocytosis was significantly higher in virus-infected patients.The significant frequency of these pathogens in patients with AG highlights the need to routinely implement laboratory investigations.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Virologia, Hospital de Clínicas, Departamento de Pediatria, Universidade Federal do Paraná, Curitiba, PR, Brasil.

ABSTRACT
Viral acute gastroenteritis (AG) is a significant cause of hospitalisation in children younger than five years. Group A rotavirus (RVA) is responsible for 30% of these cases. Following the introduction of RVA immunisation in Brazil in 2006, a decreased circulation of this virus has been observed. However, AG remains an important cause of hospitalisation of paediatric patients and only limited data are available regarding the role of other enteric viruses in these cases. We conducted a prospective study of paediatric patients hospitalised for AG. Stool samples were collected to investigate human adenovirus (HAdV), RVA, norovirus (NoV) and astrovirus (AstV). NoV typing was performed by nucleotide sequencing and phylogenetic analysis. From the 225 samples tested, 60 (26%) were positive for at least one viral agent. HAdV, NoV, RVA and AstV were detected in 16%, 8%, 6% and 0% of the samples, respectively. Mixed infections were found in nine patients: HAdV/RVA (5), HAdV/NoV (3) and HAdV/NoV/RVA (1). The frequency of fever and lymphocytosis was significantly higher in virus-infected patients. Phylogenetic analysis of NoV indicated that all of these viruses belonged to genotype GII.4. The significant frequency of these pathogens in patients with AG highlights the need to routinely implement laboratory investigations.

Show MeSH
Related in: MedlinePlus