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Water-induced modulation of Helicobacter pylori virulence properties.

Guimarães NM, Azevedo NF, Vieira MJ, Figueiredo C - Mem. Inst. Oswaldo Cruz (2014)

Bottom Line: While the influence of water in Helicobacter pylori culturability and membrane integrity has been extensively studied, there are little data concerning the effect of this environment on virulence properties.However, the microorganism is no longer effective in eliciting in vitro host cell inflammation and apoptosis, possibly due to the non-functionality of the cag type IV secretion system.These H. pylori-induced host cell responses, which are lost along with culturability, are known to increase epithelial cell turnover and, consequently, could have a deleterious effect on the initial H. pylori colonisation process.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Pathology and Immunology, Medical Faculty, University of Porto, Porto, Portugal.

ABSTRACT
While the influence of water in Helicobacter pylori culturability and membrane integrity has been extensively studied, there are little data concerning the effect of this environment on virulence properties. Therefore, we studied the culturability of water-exposed H. pylori and determined whether there was any relation with the bacterium's ability to adhere, produce functional components of pathogenicity and induce inflammation and alterations in apoptosis in an experimental model of human gastric epithelial cells. H. pylori partially retained the ability to adhere to epithelial cells even after complete loss of culturability. However, the microorganism is no longer effective in eliciting in vitro host cell inflammation and apoptosis, possibly due to the non-functionality of the cag type IV secretion system. These H. pylori-induced host cell responses, which are lost along with culturability, are known to increase epithelial cell turnover and, consequently, could have a deleterious effect on the initial H. pylori colonisation process. The fact that adhesion is maintained by H. pylori to the detriment of other factors involved in later infection stages appears to point to a modulation of the physiology of the pathogen after water exposure and might provide the microorganism with the necessary means to, at least transiently, colonise the human stomach.

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: effect of water exposure on Helicobacter pyloriculturability. After water exposure, bacteria suspension was platted on trypticsoy agar plates and incubated for seven days at 37ºC in microaerophilicconditions. The colony-forming units (CFUs) formed were counted to assess theculturability. Each experiment was performed in triplicate.
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f01: : effect of water exposure on Helicobacter pyloriculturability. After water exposure, bacteria suspension was platted on trypticsoy agar plates and incubated for seven days at 37ºC in microaerophilicconditions. The colony-forming units (CFUs) formed were counted to assess theculturability. Each experiment was performed in triplicate.

Mentions: H. pylori culturability after water exposure - The culturability ofH. pylori was evaluated after 0 h, 2 h, 6 h, 24 h and 48 h of waterexposure. Based on previous studies (Adams et al.2003, Azevedo et al. 2004), weanticipated that the longest timepoints would be sufficient to turn the bacterium intothe non-culturable state. The results obtained confirmed our expectations, as theculturability of H. pylori progressively decreased and, after 24 h ofwater exposure, H. pylori was no longer culturable (Fig. 1). The subsequent studies were performed at alltime points as well and we were able to observe the modulation of the virulenceproperties of H. pylori as the bacteria transitioned from theculturable to non-culturable state.


Water-induced modulation of Helicobacter pylori virulence properties.

Guimarães NM, Azevedo NF, Vieira MJ, Figueiredo C - Mem. Inst. Oswaldo Cruz (2014)

: effect of water exposure on Helicobacter pyloriculturability. After water exposure, bacteria suspension was platted on trypticsoy agar plates and incubated for seven days at 37ºC in microaerophilicconditions. The colony-forming units (CFUs) formed were counted to assess theculturability. Each experiment was performed in triplicate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4155841&req=5

f01: : effect of water exposure on Helicobacter pyloriculturability. After water exposure, bacteria suspension was platted on trypticsoy agar plates and incubated for seven days at 37ºC in microaerophilicconditions. The colony-forming units (CFUs) formed were counted to assess theculturability. Each experiment was performed in triplicate.
Mentions: H. pylori culturability after water exposure - The culturability ofH. pylori was evaluated after 0 h, 2 h, 6 h, 24 h and 48 h of waterexposure. Based on previous studies (Adams et al.2003, Azevedo et al. 2004), weanticipated that the longest timepoints would be sufficient to turn the bacterium intothe non-culturable state. The results obtained confirmed our expectations, as theculturability of H. pylori progressively decreased and, after 24 h ofwater exposure, H. pylori was no longer culturable (Fig. 1). The subsequent studies were performed at alltime points as well and we were able to observe the modulation of the virulenceproperties of H. pylori as the bacteria transitioned from theculturable to non-culturable state.

Bottom Line: While the influence of water in Helicobacter pylori culturability and membrane integrity has been extensively studied, there are little data concerning the effect of this environment on virulence properties.However, the microorganism is no longer effective in eliciting in vitro host cell inflammation and apoptosis, possibly due to the non-functionality of the cag type IV secretion system.These H. pylori-induced host cell responses, which are lost along with culturability, are known to increase epithelial cell turnover and, consequently, could have a deleterious effect on the initial H. pylori colonisation process.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Pathology and Immunology, Medical Faculty, University of Porto, Porto, Portugal.

ABSTRACT
While the influence of water in Helicobacter pylori culturability and membrane integrity has been extensively studied, there are little data concerning the effect of this environment on virulence properties. Therefore, we studied the culturability of water-exposed H. pylori and determined whether there was any relation with the bacterium's ability to adhere, produce functional components of pathogenicity and induce inflammation and alterations in apoptosis in an experimental model of human gastric epithelial cells. H. pylori partially retained the ability to adhere to epithelial cells even after complete loss of culturability. However, the microorganism is no longer effective in eliciting in vitro host cell inflammation and apoptosis, possibly due to the non-functionality of the cag type IV secretion system. These H. pylori-induced host cell responses, which are lost along with culturability, are known to increase epithelial cell turnover and, consequently, could have a deleterious effect on the initial H. pylori colonisation process. The fact that adhesion is maintained by H. pylori to the detriment of other factors involved in later infection stages appears to point to a modulation of the physiology of the pathogen after water exposure and might provide the microorganism with the necessary means to, at least transiently, colonise the human stomach.

Show MeSH
Related in: MedlinePlus