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Are Cristaria herculea (Middendorff, 1847) and Cristaria plicata (Leach, 1815) (Bivalvia, Unionidae) separate species?

Klishko OK, Lopes-Lima M, Froufe E, Bogan AE - Zookeys (2014)

Bottom Line: The shell morphology and the whole soft body anatomy were analysed in detail and compared with previously published information on other Cristaria spp..Additionally, a cytochrome oxidase subunit 1 (CO1) gene fragment was sequenced from foot tissue samples of selected animals, collected from the same region, and compared with published data.Moreover, a comprehensive revision of the genus Cristaria is needed, restricting the type locality and comparing topotypic specimens for both C. plicata and C. tuberculata, and including all recognized Cristaria species.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Natural Resources, Ecology and Criology, Russian Academy of Sciences Siberian Branch, Chita 672014, Russia.

ABSTRACT
The number of species in the freshwater mussel genus Cristaria Schumacher, 1817 recognized from Far East Russia has varied over the last several decades. While some authors consider the occurrence of only one species, Cristaria plicata (Leach, 1815), widespread in East Asia, others, recognize two separate species Cristaria herculea (Middendorff, 1847) and Cristaria tuberculata Schumacher, 1817 from Far East Russia, distinct from C. plicata. For the present study, freshwater mussels, identified as C. herculea, were collected in the Upper Amur basin (Transbaikalia, Russia). The shell morphology and the whole soft body anatomy were analysed in detail and compared with previously published information on other Cristaria spp.. Additionally, a cytochrome oxidase subunit 1 (CO1) gene fragment was sequenced from foot tissue samples of selected animals, collected from the same region, and compared with published data. Based upon morphological similarities of glochidia and adult morphology and anatomy as well as the mitochondrial DNA sequence analysis, we consider C. herculea as a synonym of C. plicata. Further analysis of Far East Russia C. herculea and C. tuberculata specimens using both molecular and morphological characters should be carried in the future to enhance our knowledge about the taxonomy within the Cristaria genus. Moreover, a comprehensive revision of the genus Cristaria is needed, restricting the type locality and comparing topotypic specimens for both C. plicata and C. tuberculata, and including all recognized Cristaria species.

No MeSH data available.


Phylogenetic tree obtained by Bayesian Inference and Maximum Likelihood analyses, using mtDNA fragments (CO1). Support values are given as Bayesian posterior probability above nodes and as bootstrap support below nodes, except for those within major clades, which have been omitted for clarity. Available sequences downloaded from GenBank and new sequences codes refer to Table 2.
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Figure 8: Phylogenetic tree obtained by Bayesian Inference and Maximum Likelihood analyses, using mtDNA fragments (CO1). Support values are given as Bayesian posterior probability above nodes and as bootstrap support below nodes, except for those within major clades, which have been omitted for clarity. Available sequences downloaded from GenBank and new sequences codes refer to Table 2.

Mentions: Aligned CO1 sequences had a total length of 620 bp, with 143 polymorphic and 92 parsimony informative sites. No indels and no unexpected stop codons were observed after translating all sequences to amino acids. The tree topologies resulting from the single tree recovered from ML and BI approaches were congruent, and results of both analyses are shown in Figure 8. Two major mtDNA clades were retrieved with strong support: one includes all the individuals from Cristaria plicata, including the new sequences collected for this work (Biv246 and Biv247; Fig. 8) and the other includes six individuals also originally assigned to Cristaria plicata (Jia and Li, Unpublished). However, it is obvious that the phylogeny of the Cristaria genus needs further evaluation, since these individuals are 8.9% (uncorrected p-distance) from the others, strongly indicating the existence of two different Cristaria species in this data set. Thus, this clade is here referred as Cristaria sp.


Are Cristaria herculea (Middendorff, 1847) and Cristaria plicata (Leach, 1815) (Bivalvia, Unionidae) separate species?

Klishko OK, Lopes-Lima M, Froufe E, Bogan AE - Zookeys (2014)

Phylogenetic tree obtained by Bayesian Inference and Maximum Likelihood analyses, using mtDNA fragments (CO1). Support values are given as Bayesian posterior probability above nodes and as bootstrap support below nodes, except for those within major clades, which have been omitted for clarity. Available sequences downloaded from GenBank and new sequences codes refer to Table 2.
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4155721&req=5

Figure 8: Phylogenetic tree obtained by Bayesian Inference and Maximum Likelihood analyses, using mtDNA fragments (CO1). Support values are given as Bayesian posterior probability above nodes and as bootstrap support below nodes, except for those within major clades, which have been omitted for clarity. Available sequences downloaded from GenBank and new sequences codes refer to Table 2.
Mentions: Aligned CO1 sequences had a total length of 620 bp, with 143 polymorphic and 92 parsimony informative sites. No indels and no unexpected stop codons were observed after translating all sequences to amino acids. The tree topologies resulting from the single tree recovered from ML and BI approaches were congruent, and results of both analyses are shown in Figure 8. Two major mtDNA clades were retrieved with strong support: one includes all the individuals from Cristaria plicata, including the new sequences collected for this work (Biv246 and Biv247; Fig. 8) and the other includes six individuals also originally assigned to Cristaria plicata (Jia and Li, Unpublished). However, it is obvious that the phylogeny of the Cristaria genus needs further evaluation, since these individuals are 8.9% (uncorrected p-distance) from the others, strongly indicating the existence of two different Cristaria species in this data set. Thus, this clade is here referred as Cristaria sp.

Bottom Line: The shell morphology and the whole soft body anatomy were analysed in detail and compared with previously published information on other Cristaria spp..Additionally, a cytochrome oxidase subunit 1 (CO1) gene fragment was sequenced from foot tissue samples of selected animals, collected from the same region, and compared with published data.Moreover, a comprehensive revision of the genus Cristaria is needed, restricting the type locality and comparing topotypic specimens for both C. plicata and C. tuberculata, and including all recognized Cristaria species.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Natural Resources, Ecology and Criology, Russian Academy of Sciences Siberian Branch, Chita 672014, Russia.

ABSTRACT
The number of species in the freshwater mussel genus Cristaria Schumacher, 1817 recognized from Far East Russia has varied over the last several decades. While some authors consider the occurrence of only one species, Cristaria plicata (Leach, 1815), widespread in East Asia, others, recognize two separate species Cristaria herculea (Middendorff, 1847) and Cristaria tuberculata Schumacher, 1817 from Far East Russia, distinct from C. plicata. For the present study, freshwater mussels, identified as C. herculea, were collected in the Upper Amur basin (Transbaikalia, Russia). The shell morphology and the whole soft body anatomy were analysed in detail and compared with previously published information on other Cristaria spp.. Additionally, a cytochrome oxidase subunit 1 (CO1) gene fragment was sequenced from foot tissue samples of selected animals, collected from the same region, and compared with published data. Based upon morphological similarities of glochidia and adult morphology and anatomy as well as the mitochondrial DNA sequence analysis, we consider C. herculea as a synonym of C. plicata. Further analysis of Far East Russia C. herculea and C. tuberculata specimens using both molecular and morphological characters should be carried in the future to enhance our knowledge about the taxonomy within the Cristaria genus. Moreover, a comprehensive revision of the genus Cristaria is needed, restricting the type locality and comparing topotypic specimens for both C. plicata and C. tuberculata, and including all recognized Cristaria species.

No MeSH data available.