Limits...
Are Cristaria herculea (Middendorff, 1847) and Cristaria plicata (Leach, 1815) (Bivalvia, Unionidae) separate species?

Klishko OK, Lopes-Lima M, Froufe E, Bogan AE - Zookeys (2014)

Bottom Line: The shell morphology and the whole soft body anatomy were analysed in detail and compared with previously published information on other Cristaria spp..Additionally, a cytochrome oxidase subunit 1 (CO1) gene fragment was sequenced from foot tissue samples of selected animals, collected from the same region, and compared with published data.Moreover, a comprehensive revision of the genus Cristaria is needed, restricting the type locality and comparing topotypic specimens for both C. plicata and C. tuberculata, and including all recognized Cristaria species.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Natural Resources, Ecology and Criology, Russian Academy of Sciences Siberian Branch, Chita 672014, Russia.

ABSTRACT
The number of species in the freshwater mussel genus Cristaria Schumacher, 1817 recognized from Far East Russia has varied over the last several decades. While some authors consider the occurrence of only one species, Cristaria plicata (Leach, 1815), widespread in East Asia, others, recognize two separate species Cristaria herculea (Middendorff, 1847) and Cristaria tuberculata Schumacher, 1817 from Far East Russia, distinct from C. plicata. For the present study, freshwater mussels, identified as C. herculea, were collected in the Upper Amur basin (Transbaikalia, Russia). The shell morphology and the whole soft body anatomy were analysed in detail and compared with previously published information on other Cristaria spp.. Additionally, a cytochrome oxidase subunit 1 (CO1) gene fragment was sequenced from foot tissue samples of selected animals, collected from the same region, and compared with published data. Based upon morphological similarities of glochidia and adult morphology and anatomy as well as the mitochondrial DNA sequence analysis, we consider C. herculea as a synonym of C. plicata. Further analysis of Far East Russia C. herculea and C. tuberculata specimens using both molecular and morphological characters should be carried in the future to enhance our knowledge about the taxonomy within the Cristaria genus. Moreover, a comprehensive revision of the genus Cristaria is needed, restricting the type locality and comparing topotypic specimens for both C. plicata and C. tuberculata, and including all recognized Cristaria species.

No MeSH data available.


Labial palps of Cristaria herculea (A, B, C) and of Sinanodonta sp. (D).
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4155721&req=5

Figure 6: Labial palps of Cristaria herculea (A, B, C) and of Sinanodonta sp. (D).

Mentions: A general view of the whole soft body morphology of Cristaria herculea is shown in Fig. 4. Mantle colour is creamy white, with black or brown edges (Fig. 4a). Dorsal mantle margin presents a well expressed high angle with a comb-shaped projections on the top (Fig. 4k) and a muscular anterior margin (Fig. 4l). Gills are creamy white or light brown; dorsal margin is straight to sinuous and ventral margin is moderately convex. Inner gills are much longer and higher than outer gills (Fig. 4b, c); gill length is 46–54% of shell length, gill height is 25–40% of gill length and outer gill height is 67–75% of the inner gill height. The foot is massive, creamy white and darker distally (Fig. 4d). Labial palps are triangular, creamy white to blue-grey, straight or slightly convex dorsally; straight or gently concave ventrally and bluntly pointed ventrally (Fig. 4e). Labial palp length is 3.9–4.2% of inner gill length and labial palp height 34–35% of labial palp length. Incurrent aperture (Fig. 4f) is longer than the excurrent aperture (Fig. 4g) and shorter than the supra-anal aperture (Fig. 4h). Supra-anal aperture opening is located from the dorsal margin of the posterior adductor muscle (Fig. 4i) to the posterior dorsal edge of the posterior mantle wing. Supra-anal aperture length is 20–25% of the shell length or double the length of the incurrent aperture; it is creamy white to pearly white inside, with a very thin yellow-brown marginal band. Mantle bridge (Fig. 4j) separates the excurrent from the supra-anal aperture and is 8–10% of the supra-anal aperture length. Incurrent aperture length is 11–13% of the shell length, is creamy white to light tan within, with a combination of orange, brown and black basal to the papillae and to the bands margin which may present a reticular pattern. Excurrent aperture length is 46% of incurrent aperture length, colour is creamy white within with black or dark brown edges basally, margin papillate; have irregular mottled pigmentation of some combination of dark brown and orange (Fig. 5A–C). Papillae of the incurrent aperture are located in 3–4 rows, linear-fusiform in shape, mostly simple, with thickening of the papilla basement in the first and second medial rows, dark-orange; papillae of outer or lateral rows are shorter and more numerous (Fig. 5C). Labial palps of Cristaria herculea and Sinanodonta sp. are morphologically distinct (Fig. 6A–D). The anterior acuminate edges of Sinanodonta sp. labial palps are not completely attached to the mantle (Fig. 6D) in contrast with those on Cristaria herculea (Fig. 6A–C). The distinctive feature of the genus Cristaria within the tribe Anodontini is the posterior dorsal mantle wing and projections (Fig. 7). The comb-shaped projections are dorsal extensions of the mantle that penetrate into the cavities of shell wing, to provide for the wing growth.


Are Cristaria herculea (Middendorff, 1847) and Cristaria plicata (Leach, 1815) (Bivalvia, Unionidae) separate species?

Klishko OK, Lopes-Lima M, Froufe E, Bogan AE - Zookeys (2014)

Labial palps of Cristaria herculea (A, B, C) and of Sinanodonta sp. (D).
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4155721&req=5

Figure 6: Labial palps of Cristaria herculea (A, B, C) and of Sinanodonta sp. (D).
Mentions: A general view of the whole soft body morphology of Cristaria herculea is shown in Fig. 4. Mantle colour is creamy white, with black or brown edges (Fig. 4a). Dorsal mantle margin presents a well expressed high angle with a comb-shaped projections on the top (Fig. 4k) and a muscular anterior margin (Fig. 4l). Gills are creamy white or light brown; dorsal margin is straight to sinuous and ventral margin is moderately convex. Inner gills are much longer and higher than outer gills (Fig. 4b, c); gill length is 46–54% of shell length, gill height is 25–40% of gill length and outer gill height is 67–75% of the inner gill height. The foot is massive, creamy white and darker distally (Fig. 4d). Labial palps are triangular, creamy white to blue-grey, straight or slightly convex dorsally; straight or gently concave ventrally and bluntly pointed ventrally (Fig. 4e). Labial palp length is 3.9–4.2% of inner gill length and labial palp height 34–35% of labial palp length. Incurrent aperture (Fig. 4f) is longer than the excurrent aperture (Fig. 4g) and shorter than the supra-anal aperture (Fig. 4h). Supra-anal aperture opening is located from the dorsal margin of the posterior adductor muscle (Fig. 4i) to the posterior dorsal edge of the posterior mantle wing. Supra-anal aperture length is 20–25% of the shell length or double the length of the incurrent aperture; it is creamy white to pearly white inside, with a very thin yellow-brown marginal band. Mantle bridge (Fig. 4j) separates the excurrent from the supra-anal aperture and is 8–10% of the supra-anal aperture length. Incurrent aperture length is 11–13% of the shell length, is creamy white to light tan within, with a combination of orange, brown and black basal to the papillae and to the bands margin which may present a reticular pattern. Excurrent aperture length is 46% of incurrent aperture length, colour is creamy white within with black or dark brown edges basally, margin papillate; have irregular mottled pigmentation of some combination of dark brown and orange (Fig. 5A–C). Papillae of the incurrent aperture are located in 3–4 rows, linear-fusiform in shape, mostly simple, with thickening of the papilla basement in the first and second medial rows, dark-orange; papillae of outer or lateral rows are shorter and more numerous (Fig. 5C). Labial palps of Cristaria herculea and Sinanodonta sp. are morphologically distinct (Fig. 6A–D). The anterior acuminate edges of Sinanodonta sp. labial palps are not completely attached to the mantle (Fig. 6D) in contrast with those on Cristaria herculea (Fig. 6A–C). The distinctive feature of the genus Cristaria within the tribe Anodontini is the posterior dorsal mantle wing and projections (Fig. 7). The comb-shaped projections are dorsal extensions of the mantle that penetrate into the cavities of shell wing, to provide for the wing growth.

Bottom Line: The shell morphology and the whole soft body anatomy were analysed in detail and compared with previously published information on other Cristaria spp..Additionally, a cytochrome oxidase subunit 1 (CO1) gene fragment was sequenced from foot tissue samples of selected animals, collected from the same region, and compared with published data.Moreover, a comprehensive revision of the genus Cristaria is needed, restricting the type locality and comparing topotypic specimens for both C. plicata and C. tuberculata, and including all recognized Cristaria species.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Natural Resources, Ecology and Criology, Russian Academy of Sciences Siberian Branch, Chita 672014, Russia.

ABSTRACT
The number of species in the freshwater mussel genus Cristaria Schumacher, 1817 recognized from Far East Russia has varied over the last several decades. While some authors consider the occurrence of only one species, Cristaria plicata (Leach, 1815), widespread in East Asia, others, recognize two separate species Cristaria herculea (Middendorff, 1847) and Cristaria tuberculata Schumacher, 1817 from Far East Russia, distinct from C. plicata. For the present study, freshwater mussels, identified as C. herculea, were collected in the Upper Amur basin (Transbaikalia, Russia). The shell morphology and the whole soft body anatomy were analysed in detail and compared with previously published information on other Cristaria spp.. Additionally, a cytochrome oxidase subunit 1 (CO1) gene fragment was sequenced from foot tissue samples of selected animals, collected from the same region, and compared with published data. Based upon morphological similarities of glochidia and adult morphology and anatomy as well as the mitochondrial DNA sequence analysis, we consider C. herculea as a synonym of C. plicata. Further analysis of Far East Russia C. herculea and C. tuberculata specimens using both molecular and morphological characters should be carried in the future to enhance our knowledge about the taxonomy within the Cristaria genus. Moreover, a comprehensive revision of the genus Cristaria is needed, restricting the type locality and comparing topotypic specimens for both C. plicata and C. tuberculata, and including all recognized Cristaria species.

No MeSH data available.