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MiR-506 suppresses proliferation of hepatoma cells through targeting YAP mRNA 3'UTR.

Wang Y, Cui M, Sun BD, Liu FB, Zhang XD, Ye LH - Acta Pharmacol. Sin. (2014)

Bottom Line: Bioinformatics analysis revealed that YAP mRNA might be one of the targets of miR-506, and miR-506 in HCC tissues was found to be negatively correlated with YAP (r=-0.605).Furthermore, miR-506 significantly inhibited the proliferation of HepG2 and H7402 cells, while anti-miR-506 enhanced the cell proliferation, which was blocked by YAP siRNA.MiR-506 suppresses the proliferation of hepatoma cells by targeting YAP mRNA.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Medicinal Chemical Biology, Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071, China.

ABSTRACT

Aim: MiR-506 is a miRNA involved in carcinogenesis of several kinds of cancer. In this study, we explored whether miR-506 played a critical role in hepatocellular carcinoma (HCC).

Methods: Twenty HCC and adjacent normal liver tissue samples were collected. Human hepatoma cell lines HepG2 and H7402 were used for in vitro studies. The expression of miR-506 and transcriptional co-activator YAP was examined using qRT-PCR. Western blot analysis was used to measure the expression of YAP and its target genes. Luciferase reporter gene assay was used to identify YAP as a target gene of miR-506. MTT and EdU assays were carried out for functional analysis.

Results: The expression of miR-506 was significantly lower in HCC than in adjacent normal liver tissues. Bioinformatics analysis revealed that YAP mRNA might be one of the targets of miR-506, and miR-506 in HCC tissues was found to be negatively correlated with YAP (r=-0.605). In both HepG2 and H7402 cells, miR-506 dose-dependently down-regulated YAP and its target genes c-Myc and the connective tissue growth factor (CTGF). Luciferase reporter gene assays demonstrated that miR-506 targeted the wild type 3'UTR of YAP mRNA, but not a 3'UTR with a mutant seed site. Furthermore, miR-506 significantly inhibited the proliferation of HepG2 and H7402 cells, while anti-miR-506 enhanced the cell proliferation, which was blocked by YAP siRNA.

Conclusion: MiR-506 suppresses the proliferation of hepatoma cells by targeting YAP mRNA.

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Related in: MedlinePlus

MiR-506 suppresses the proliferation of hepatoma cells by inhibiting YAP. (A and B) HepG2 and H7402 cells were transfected with NC (negative control), miR-506, anti-miR-506, or YAP siRNA. The effect of miR-506 on cell proliferation was determined by MTT and EdU assays. Statistically significant differences are indicated: bP<0.05 and cP<0.01; Student's t test.
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fig4: MiR-506 suppresses the proliferation of hepatoma cells by inhibiting YAP. (A and B) HepG2 and H7402 cells were transfected with NC (negative control), miR-506, anti-miR-506, or YAP siRNA. The effect of miR-506 on cell proliferation was determined by MTT and EdU assays. Statistically significant differences are indicated: bP<0.05 and cP<0.01; Student's t test.

Mentions: To examine the potential role of miR-506 in tumorigenesis, we sought to determine whether miR-506 affects the proliferation of hepatoma cells using MTT and EdU assays. Our data indicated that the proliferation of HepG2 and H7402 cells was decreased when the cells were treated with miR-506, while the treatment with anti-miR-506 increased the proliferation. The treatment with YAP siRNA was able to block the anti-miR-506-enhanced proliferation (Figure 4), suggesting that miR-506 suppresses the proliferation of hepatoma cells by modulating YAP. Therefore, our observations suggest that miR-506 is able to inhibit the proliferation of hepatoma cells through direct targeting of YAP mRNA.


MiR-506 suppresses proliferation of hepatoma cells through targeting YAP mRNA 3'UTR.

Wang Y, Cui M, Sun BD, Liu FB, Zhang XD, Ye LH - Acta Pharmacol. Sin. (2014)

MiR-506 suppresses the proliferation of hepatoma cells by inhibiting YAP. (A and B) HepG2 and H7402 cells were transfected with NC (negative control), miR-506, anti-miR-506, or YAP siRNA. The effect of miR-506 on cell proliferation was determined by MTT and EdU assays. Statistically significant differences are indicated: bP<0.05 and cP<0.01; Student's t test.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4155531&req=5

fig4: MiR-506 suppresses the proliferation of hepatoma cells by inhibiting YAP. (A and B) HepG2 and H7402 cells were transfected with NC (negative control), miR-506, anti-miR-506, or YAP siRNA. The effect of miR-506 on cell proliferation was determined by MTT and EdU assays. Statistically significant differences are indicated: bP<0.05 and cP<0.01; Student's t test.
Mentions: To examine the potential role of miR-506 in tumorigenesis, we sought to determine whether miR-506 affects the proliferation of hepatoma cells using MTT and EdU assays. Our data indicated that the proliferation of HepG2 and H7402 cells was decreased when the cells were treated with miR-506, while the treatment with anti-miR-506 increased the proliferation. The treatment with YAP siRNA was able to block the anti-miR-506-enhanced proliferation (Figure 4), suggesting that miR-506 suppresses the proliferation of hepatoma cells by modulating YAP. Therefore, our observations suggest that miR-506 is able to inhibit the proliferation of hepatoma cells through direct targeting of YAP mRNA.

Bottom Line: Bioinformatics analysis revealed that YAP mRNA might be one of the targets of miR-506, and miR-506 in HCC tissues was found to be negatively correlated with YAP (r=-0.605).Furthermore, miR-506 significantly inhibited the proliferation of HepG2 and H7402 cells, while anti-miR-506 enhanced the cell proliferation, which was blocked by YAP siRNA.MiR-506 suppresses the proliferation of hepatoma cells by targeting YAP mRNA.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Medicinal Chemical Biology, Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071, China.

ABSTRACT

Aim: MiR-506 is a miRNA involved in carcinogenesis of several kinds of cancer. In this study, we explored whether miR-506 played a critical role in hepatocellular carcinoma (HCC).

Methods: Twenty HCC and adjacent normal liver tissue samples were collected. Human hepatoma cell lines HepG2 and H7402 were used for in vitro studies. The expression of miR-506 and transcriptional co-activator YAP was examined using qRT-PCR. Western blot analysis was used to measure the expression of YAP and its target genes. Luciferase reporter gene assay was used to identify YAP as a target gene of miR-506. MTT and EdU assays were carried out for functional analysis.

Results: The expression of miR-506 was significantly lower in HCC than in adjacent normal liver tissues. Bioinformatics analysis revealed that YAP mRNA might be one of the targets of miR-506, and miR-506 in HCC tissues was found to be negatively correlated with YAP (r=-0.605). In both HepG2 and H7402 cells, miR-506 dose-dependently down-regulated YAP and its target genes c-Myc and the connective tissue growth factor (CTGF). Luciferase reporter gene assays demonstrated that miR-506 targeted the wild type 3'UTR of YAP mRNA, but not a 3'UTR with a mutant seed site. Furthermore, miR-506 significantly inhibited the proliferation of HepG2 and H7402 cells, while anti-miR-506 enhanced the cell proliferation, which was blocked by YAP siRNA.

Conclusion: MiR-506 suppresses the proliferation of hepatoma cells by targeting YAP mRNA.

Show MeSH
Related in: MedlinePlus