Assemblages: functional units formed by cellular phase separation.
Bottom Line: The partitioning of intracellular space beyond membrane-bound organelles can be achieved with collections of proteins that are multivalent or contain low-complexity, intrinsically disordered regions.These proteins can undergo a physical phase change to form functional granules or other entities within the cytoplasm or nucleoplasm that collectively we term "assemblage." Intrinsically disordered proteins (IDPs) play an important role in forming a subset of cellular assemblages by promoting phase separation.Recent work points to an involvement of assemblages in disease states, indicating that intrinsic disorder and phase transitions should be considered in the development of therapeutics.
Affiliation: Department of Oncology, Georgetown University, Washington, DC 20057 firstname.lastname@example.org.Show MeSH
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Mentions: Although FG repeats are a broad class of Nup, sequence variations such as FxFG and FLFG, along with FG, are differentially distributed at the cytoplasmic, intra-NPC, or nucleoplasmic regions of the NPC (Atkinson et al., 2013). A mutagenesis analysis of the role of FG repeat Nups in yeast suggests that multiple pathways exist for transport through the nuclear pore (Strawn et al., 2004). The FG Nups are structurally heterogeneous, adopting a spectrum of disordered conformational states that range from collapsed coils to highly extended configurations (Yamada et al., 2010), leading to a zonal model of the nuclear pore (Fig. 2). Additional investigations support a critical role for FG nucleoporins in the transport of mRNA, including relative specificities for GLFG and FxFG repeat sequences (Terry and Wente, 2007).
Affiliation: Department of Oncology, Georgetown University, Washington, DC 20057 email@example.com.