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BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior.

Corrigan L, Redhai S, Leiblich A, Fan SJ, Perera SM, Patel R, Gandy C, Wainwright SM, Morris JF, Hamdy F, Goberdhan DC, Wilson C - J. Cell Biol. (2014)

Bottom Line: Male reproductive glands secrete signals into seminal fluid to facilitate reproductive success.Exosome release was required to inhibit female remating behavior, suggesting that exosomes are downstream effectors of BMP signaling.These results demonstrate a new function for the MVB-exosome pathway in the reproductive tract that appears to be conserved across evolution.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology, Anatomy and Genetics and Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX1 3QX, England, UK.

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ALiX and Hrs have different effects on SC growth. (A) Growth of SCs relative to MCs in 6-d-old males expressing different transgenes in SCs, as measured by the ratio of SC to MC nuclear size and normalized to controls. Values inside bars show number of males tested (n). DN, dominant negative. (B–D) SCs in 6-d-old males expressing either GFP linked to a nuclear localization sequence (GFPNLS) alone (B) or in combination with either Hrs-RNAi (C) or ALiX-RNAi (D), under the control of the esgF/Ots driver. Control SC nuclei are smaller than MC nuclei at eclosion but grow to be larger by 6 d (B). Data for A were analyzed using the Student’s t test or Mann–Whitney U test (*, P < 0.05; **, P < 0.01; ***, P < 0.001, n = 10) after a Shapiro–Wilk test for normality. Error bars indicate ±SE. Bars, 10 µm.
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fig9: ALiX and Hrs have different effects on SC growth. (A) Growth of SCs relative to MCs in 6-d-old males expressing different transgenes in SCs, as measured by the ratio of SC to MC nuclear size and normalized to controls. Values inside bars show number of males tested (n). DN, dominant negative. (B–D) SCs in 6-d-old males expressing either GFP linked to a nuclear localization sequence (GFPNLS) alone (B) or in combination with either Hrs-RNAi (C) or ALiX-RNAi (D), under the control of the esgF/Ots driver. Control SC nuclei are smaller than MC nuclei at eclosion but grow to be larger by 6 d (B). Data for A were analyzed using the Student’s t test or Mann–Whitney U test (*, P < 0.05; **, P < 0.01; ***, P < 0.001, n = 10) after a Shapiro–Wilk test for normality. Error bars indicate ±SE. Bars, 10 µm.

Mentions: Because BMP signaling drives SC growth, as measured by relative size of SC versus neighboring MC nuclei (Leiblich et al., 2012), we tested whether exosome formation or secretion might be involved in this. Interestingly, although nuclei of SCs expressing either ALiX-RNAi or Rab11DN were reduced in size, nuclear size was increased in Hrs-RNAi–expressing SCs (Fig. 9). This suggests that ESCRT-dependent ILV formation is not required for SC growth.


BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior.

Corrigan L, Redhai S, Leiblich A, Fan SJ, Perera SM, Patel R, Gandy C, Wainwright SM, Morris JF, Hamdy F, Goberdhan DC, Wilson C - J. Cell Biol. (2014)

ALiX and Hrs have different effects on SC growth. (A) Growth of SCs relative to MCs in 6-d-old males expressing different transgenes in SCs, as measured by the ratio of SC to MC nuclear size and normalized to controls. Values inside bars show number of males tested (n). DN, dominant negative. (B–D) SCs in 6-d-old males expressing either GFP linked to a nuclear localization sequence (GFPNLS) alone (B) or in combination with either Hrs-RNAi (C) or ALiX-RNAi (D), under the control of the esgF/Ots driver. Control SC nuclei are smaller than MC nuclei at eclosion but grow to be larger by 6 d (B). Data for A were analyzed using the Student’s t test or Mann–Whitney U test (*, P < 0.05; **, P < 0.01; ***, P < 0.001, n = 10) after a Shapiro–Wilk test for normality. Error bars indicate ±SE. Bars, 10 µm.
© Copyright Policy - openaccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4151142&req=5

fig9: ALiX and Hrs have different effects on SC growth. (A) Growth of SCs relative to MCs in 6-d-old males expressing different transgenes in SCs, as measured by the ratio of SC to MC nuclear size and normalized to controls. Values inside bars show number of males tested (n). DN, dominant negative. (B–D) SCs in 6-d-old males expressing either GFP linked to a nuclear localization sequence (GFPNLS) alone (B) or in combination with either Hrs-RNAi (C) or ALiX-RNAi (D), under the control of the esgF/Ots driver. Control SC nuclei are smaller than MC nuclei at eclosion but grow to be larger by 6 d (B). Data for A were analyzed using the Student’s t test or Mann–Whitney U test (*, P < 0.05; **, P < 0.01; ***, P < 0.001, n = 10) after a Shapiro–Wilk test for normality. Error bars indicate ±SE. Bars, 10 µm.
Mentions: Because BMP signaling drives SC growth, as measured by relative size of SC versus neighboring MC nuclei (Leiblich et al., 2012), we tested whether exosome formation or secretion might be involved in this. Interestingly, although nuclei of SCs expressing either ALiX-RNAi or Rab11DN were reduced in size, nuclear size was increased in Hrs-RNAi–expressing SCs (Fig. 9). This suggests that ESCRT-dependent ILV formation is not required for SC growth.

Bottom Line: Male reproductive glands secrete signals into seminal fluid to facilitate reproductive success.Exosome release was required to inhibit female remating behavior, suggesting that exosomes are downstream effectors of BMP signaling.These results demonstrate a new function for the MVB-exosome pathway in the reproductive tract that appears to be conserved across evolution.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology, Anatomy and Genetics and Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX1 3QX, England, UK.

Show MeSH
Related in: MedlinePlus